Method for breeding salt-tolerance mutant of sweet-potato

A technology of mutants and sweet potatoes, applied in horticultural methods, botany equipment and methods, biochemical equipment and methods, etc., can solve problems such as salt tolerance identification of regenerated plants, and achieve easy mastery, good repeatability, and wide application range Effect

Inactive Publication Date: 2007-07-25
CHINA AGRI UNIV
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Problems solved by technology

[0004] So far, there are few reports on the screening methods of sweet potato salt-tolerant mutants, only Li Aixian et al. The result of the study was to treat sweet potato embryogenic suspension cells wit

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  • Method for breeding salt-tolerance mutant of sweet-potato
  • Method for breeding salt-tolerance mutant of sweet-potato

Examples

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Embodiment 1

[0048] Embodiment 1, the screening of the salt-tolerant mutant of sweet potato variety Lizixiang

[0049] 1. Prepare the embryogenic suspension cells of Lizixiang according to the following method: (1) Get about 30mm of the stem tip of the vigorously growing plant in the greenhouse, wash it with tap water, disinfect it with 70% alcohol for 10 seconds, then disinfect it with 2% sodium hypochlorite for 5 minutes, Finally, wash it with sterile distilled water; (2) strip the shoot tip of about 0.5mm long under the dissecting microscope, and cultivate it in the addition of 2.0mg / L 2,4-D, 3.0% sucrose at 26-28°C in the dark and 0.8% agar, pH5.8 MS medium, after 4-8 weeks of culture, the ideal embryogenic callus will be formed; (3) the embryogenic callus will be broken into cell clusters with a stainless steel mesh screen with a pore size of 100 μm , and then transferred to a 100mL Erlenmeyer flask containing 2.0mg / L 2,4-D and 3% sucrose, pH5.8 20mL MS medium, placed on a horizontal ...

Embodiment 2

[0059] Example 2, Screening of salt-tolerant mutants of sweet potato variety Xushu 18

[0060] 1. Prepare the embryogenic suspension cells of Xushu 18 according to the following method: (1) Take about 30 mm of the shoot tip of the vigorously growing plant in the greenhouse, wash it with tap water, disinfect it with 70% alcohol for 10 seconds, and then disinfect it with 2% sodium hypochlorite for 5 minutes , and finally washed with sterile distilled water; (2) under a dissecting microscope, strip the shoot tip with a length of about 0.5mm, and culture it in the addition of 2.0mg / L 2,4-D, 3.0% at 26-28°C in the dark On MS medium with sucrose and 0.8% agar, pH5.8, the ideal embryogenic callus will be formed after 4-8 weeks of culture; (3) The embryogenic callus will be broken into cells with a stainless steel mesh screen with a pore size of 100 μm Then transferred to a 100mL Erlenmeyer flask containing 2.0mg / L 2,4-D and 3% sucrose, pH 5.8 20mL MS medium, placed on a horizontal sh...

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Abstract

A method for screening the salt-resistant mutant of sweet potato includes such steps as secondary culture of suspended embryonic cells of sweet potato, irradiating them by Co gamma-ray, sequentially culturing in liquid culture media A, B and C, culturing in solid culture medium A to obtain calli, sequentially culturing in solid culture media B, C and D to obtain plantlet, culturing in basic MS culture medium, culturing in liquid culture medium D, and culturing in Hoagland solution containing NaCl.

Description

technical field [0001] The invention relates to a method for screening sweet potato salt-tolerant mutants. Background technique [0002] Soil salinization is a global problem that threatens agricultural production, with about 1 billion hectares worldwide 2 The saline soil occupies about 7.6% of the world's land area. my country has about 20 million hm 2 Saline soil, and with the continuous expansion of irrigated area and protected cultivation area, the secondary salinization of soil is also becoming more and more serious. Improving the salt tolerance of plants is an important way to carry out biological treatment and comprehensive development and utilization of saline soil. [0003] Sweet potato is an important food, feed, industrial raw material and new energy crop. A considerable part of sweet potato in my country is planted in coastal slopes and hilly arid areas. Due to the high salt content of the soil in these areas, and the serious return of salt in the dry area soi...

Claims

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Application Information

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IPC IPC(8): A01H1/06C12N13/00C12N5/04A01G31/00A01G7/00A01H3/00A01H5/00
CPCY02A40/135
Inventor 刘庆昌何绍贞翟红王玉萍韩元凤
Owner CHINA AGRI UNIV
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