Reactor
A reactor and reaction tank technology, applied in the field of reactors, can solve the problems of shortened service life, large bed pressure drop, difficulty in feeding materials and gas ventilation, etc. Wide range of effects
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Examples
example 1
[0056] Example 1. Suspension agitation damages the immobilized enzyme carrier
[0057] Using the reactor shown in Figure 4, the currently widely used granular immobilized enzyme resin carrier Sepabeads EC-EP (Grazu V, Abian O, Mateo C, Batista-Viera F, Fernandez-Lafuente R, Guisan JM. Biotechnol Bioeng .2005,90:597-605; Grazu V, Abian O, Mateo C, Batista-Viera F, Fernandez-LafuenteR, Guisan JM.Biomacromolecules.2003,4:1495-1501) and granular immobilized glucose isomerase, respectively Carry out network disk stirring (that is, as mentioned above, the granular immobilized enzyme resin carrier and the immobilized glucose isomerase are respectively loaded into the container 53, and the container 53 follows the rotating shaft 21 for the stirring effect obtained by centrifugal rotation) and suspension stirring test , to compare the degree of damage to the resin carrier. Sepabeads EC-EP was purchased from Italy Resindion S.R.L company. Granular immobilized glucose isomerase was pur...
example 2
[0064] Example 2. The whole piece of open-pore immobilized cells is used as a stirring paddle for continuous stirring reaction to prepare high fructose syrup [1]
[0065] Glucose isomerase catalyzes the reaction that converts glucose into fructose. The vector pRSET-lac-MGI4-35-kan expressing glucose isomerase was prepared as follows. According to the sequence design of pGEMT-Easy (Promega), PCR primers are specifically: upstream primer RBS-NdeI: 5'-CATATGTATATCTCCTTCTTGTGTGAAATTG-3'; downstream primer RBS-AlwNI:
[0066] 5'-CAGTGGCTGCTGCCAGTGGCGATAAGTC-3'. Using pGEMT-Easy (Promega) as a template, PCR was performed with the above primers, and a 755bp product was amplified. The PCR conditions were: 50ng pGEMT-Easy (Promega), 0.4 μM RBS-NdeI, 0.4 μM RBS-AlwNI, 50 μM dATP, 50 μM dTTP, 50 μM dCTP, 50 μM dGTP, 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 10 mM ( NH 4 ) 2 SO 4 , 2mM MgSO 4 , 0.1% Triton X-100, 2.5U Pfu DNA polymerase (Promega), the reaction volume was adjusted to 50 μ...
example 3
[0074] Example 3. The whole piece of open-pore immobilized cells is used as a stirring paddle for continuous stirring reaction in series to prepare high fructose syrup
[0075] The preparation of the glucose isomerase-immobilized cell sheet, the determination of its activity, its installation on the reaction tank, the operation of the isomerization reaction and the reaction tank, etc. basically refer to Example 2. In this example, 50 grams of glucose isomerase-immobilized cell sheets were prepared, and the enzyme activity was 5,298 units / gram. Adopt 2 reaction tanks as shown in Figure 4, the reaction tank I and the reaction tank II, respectively fill 23.5 grams of specific activity in the grid tray 20 with immobilized cell sheet belts of 5,298 units / gram, react separately respectively, its operation Same as example 2. When the flow rate of the reaction tank I was recorded at 7.1 g / min, the optical rotation (22°C) was -8.68 (equivalent to 48.68% fructose conversion rate); when...
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Description
Claims
Application Information
- IPC
- B01J8/10
- Inventors
- 王骏; 金彩科