Fluorescent probe for detecting ultra-oxygen anion free radical, synthesis method and use

A technology of superoxide anion and fluorescent probe, which is applied in the field of biological detection technology and clinical medical detection, can solve the problems of short free radical life, difficult detection, and many types, and achieve stable performance, no toxic and side effects, and small toxic and side effects Effect
CN101012207AInactive Publication Date: 2007-08-08SHANDONG NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG NORMAL UNIV
Publication Date
2007-08-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a new typed fluorescent probe and synthesizing method and application to detect hyperoxygen anion free radical in the cell selectively, which comprises the following steps: dissolving aromatic o-aminophenyl-sulfhydrate or aromatic o-aminophenyl-sulfhydrate derivant with weight rate at 1:2 -3.5 into composite solution with butanol and benzene with bulk rate at 1:0.3-0.6; heating the composite liquid to reflux 3-5h; decompressing to remove solvent; obtaining rough product; recrystallizing through alcohol; drying in the vacuum; obtaining pure product.
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Description

Technical field:

[0001] The invention relates to the fields of biological detection technology and clinical medical detection, in particular to a series of fluorescent probes for highly selective detection of intracellular superoxide anion free radicals; at the same time, it relates to a synthesis method of the fluorescent probe; in addition, it also relates to the fluorescent The purpose of the probe. Background technique:

[0002] Numerous active oxygen free radicals are produced in the process of biological metabolism, including superoxide anion free radicals (O 2 - ), hydrogen peroxide (H 2 o 2 ), hydroxyl radicals (HO·), nitric oxide (NO), peroxynitroso (ONOO - ), etc., these free radicals are related to the pathogenesis of cancer and inflammation, tissue peroxidation, protein cross-linking denaturation, nucleic acid damage and signal transduction (Wiseman, H.; Halliwell, B.Biochem.J.1996, 313, 17-29; McCord , J.M.; Science.1974, 185, 529-531; Dobashi, K.; Ghosh, B...

Claims

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