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Process of analyzing descending differentiated fatty acid type of Ralstonia solanacearum

A technology of Ralstia solanacearum and analysis method, which is applied in the field of analysis of fatty acid types differentiated under Ralstia solanacearum species, and can solve problems such as inability to distinguish Ralstia solanacearum strains

Inactive Publication Date: 2007-08-22
BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

As pointed out in the literature "Genomicfingerprinting of Ralstonia solanacearum biovar 3 in Thai landby using pulsed-field gel electrophoresis", "Studies on classification and antiserotypes of monoclonal antibodies against Pseudomonas solanacearum" and "Studies on the comparison of serotyping and other methods from Pulse an berry methods of doping" Analysis of serotype, lysogeny, and genotype cannot distinguish strains of R. solanacearum with different virulence

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  • Process of analyzing descending differentiated fatty acid type of Ralstonia solanacearum
  • Process of analyzing descending differentiated fatty acid type of Ralstonia solanacearum
  • Process of analyzing descending differentiated fatty acid type of Ralstonia solanacearum

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Embodiment Construction

[0029] 1. Screening of Fatty Acid Stratification Factors of R. solanacearum

[0030] 1. Extraction and gas chromatography analysis of fatty acids of R. solanacearum

[0031] Reagents used for fatty acid extraction: including fatty acid methyl ester mixture standard sample, colony collection medium (TSB medium), composed of tryptone soybean broth, agar and water, saponification reagent is a mixed solution of sodium hydroxide and methanol, methylation The reagent is a mixture of hydrochloric acid and methanol, the extraction reagent is a mixture of n-hexane and methyl tert-butyl ether, and the washing reagent is sodium hydrogenotrophic solution.

[0032] First collect bacterium colony, release fatty acid after saponifying bacterial cell with saponification reagent, methylate fatty acid with methylation reagent again, then finish the extraction of 40 strains of Ralstia solanacearum fatty acid through basic washing after extraction with fatty acid methyl ester, in the following P...

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Abstract

The process of analyzing down differentiated fatty acid type of Ralstonia solanacearum includes screening Ralstonia solanacearum fatty acid laminating factor and establishing judgment models. By means of screening three characteristic factors, are established the following judgment models: Y1=-16.3353+0.0012X1+0.0056X9-0.0016X13, Y2=-3.4928+0.0002X1+0.0003X9+0.0025X13, and Y3=-9.1550-0.0003X1+0.0039X9+0.0042X13. The said analysis process can identify the fatty acid type of Ralstonia solanacearum and its pathogenicity, and provides theoretic foundation for the identification of the down differentiated fatty acid type of Ralstonia solanacearum related to pathogenicity.

Description

technical field [0001] The invention relates to a method for analyzing fatty acid types differentiated under the species of R. solanacearum. Background technique [0002] Bacterial wilt is a devastating soil-borne disease caused by the bacterium Ralstonia solanacearum. As stated in the document "Bacterial wilt of groundnut", R. solanacearum can harm more than 300 species of plants in 44 families, causing huge economic losses in agricultural and forestry production. R. solanacearum has great differences in host range, geographical distribution, pathogenic characteristics, epidemic characteristics, and subspecies differentiation, and has obvious polymorphism. The subspecific differentiation of Ralstia solanacearum is complicated, and traditional identification methods mainly refer to the literature "Identification of open reading frames unique to a select agent" from the physiological race (race), and the biochemical type (biovar) from the literature "Survi val of Ralstonia s...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N30/02G01N33/68
Inventor 刘波蓝江林朱育菁车建美林营志林抗美苏明星阮传清葛慈斌周先治刘芸
Owner BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
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