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Method for preparing high-purity Momordia grosvenori aglycone from Momordia grosvenori

A mogroside and Luo Han Guo technology, applied in food preparation, steroids, organic chemistry and other directions, can solve problems such as complicated operation and toxicity, and achieve the effects of simplified process, low production cost and high purity

Inactive Publication Date: 2007-09-05
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses macroporous resin to remove impurities, and the operation is cumbersome; reversed-phase chromatography is eluted with acetone-water, which has certain toxicity; the purity and yield have certain limitations

Method used

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  • Method for preparing high-purity Momordia grosvenori aglycone from Momordia grosvenori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] This embodiment 1 is implemented under the following conditions of implementation and technical requirements:

[0018] 1. Add 1Kg of Luo Han Guo dried fruit, add 5L of 71% industrial ethanol to reflux for extraction for 2 hours, filter, recover ethanol from the filtrate (can be used again for medicinal material extraction), and obtain 94g of alcoholic extract.

[0019] 2. Take the alcoholic extract, add 470mL of water to suspend, then extract with 235mL of water-saturated ethyl acetate, separate the ethyl acetate layer, and recover ethyl acetate under reduced pressure (which can be used again for extraction) to obtain ethyl acetate Ester extract 13g.

[0020] 3. The aqueous solution extracted with ethyl acetate was further extracted with n-butanol saturated with water, and the dosage was 235 mL. The n-butanol was separated, and the n-butanol was recovered under reduced pressure (which could be used for extraction again) to obtain 15 g of n-butanol extract.

[0021] 4....

Embodiment 2

[0025] This embodiment 2 is implemented under the following conditions of implementation and technical requirements:

[0026] 1. Add 1Kg of Luo Han Guo dried fruit, add 10L of 95% industrial ethanol to reflux and extract for 6 hours, filter, and recover ethanol from the filtrate (which can be used again for medicinal material extraction) to obtain an extract. Repeat the above operation 2 times to obtain 168g of alcoholic extract.

[0027] 2. Take the alcoholic extract, add 1680mL of water to suspend it, then extract with 1680mL of water-saturated ethyl acetate, separate the ethyl acetate layer, and reclaim the ethyl acetate under reduced pressure (which can be used again for extraction) to obtain the extract. Repeat the above operation 2 times, combine the extracts to obtain 21g of ethyl acetate extract.

[0028] 3. The aqueous solution extracted with ethyl acetate was further extracted with n-butanol saturated with water, and the dosage was 1680 mL. The n-butanol is separat...

Embodiment 3

[0033] This embodiment 3 is implemented under the following conditions of implementation and technical requirements:

[0034] 1. Add 7.5L of 80% industrial ethanol to 1Kg of dried Luo Han Guo, reflux for extraction for 4 hours, filter, and recover ethanol from the filtrate (which can be used again for medicinal material extraction) to obtain an extract. Repeat the above operation once to obtain 133g of alcoholic extract.

[0035] 2. Take the alcoholic extract, add 998mL of water to suspend it, then extract it with 749mL of water-saturated ethyl acetate, separate the ethyl acetate layer, and reclaim the ethyl acetate under reduced pressure (which can be used again for extraction) to obtain the extract. Repeat the above operation once to obtain 18 g of ethyl acetate extract.

[0036] 3. The aqueous solution extracted with ethyl acetate was further extracted with n-butanol saturated with water, and the dosage was 749 mL. Isolate n-butanol, recover n-butanol (can be used for ext...

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Abstract

A process for preparing high-purity momordicoside from fructus momordicae includes such steps as pulverizing, reflux extracting in alcohol, filtering to obtain extract A, suspending it in water, extracting in ethyl acetate 1-3 times, recovering ethyl acetate mother liquid, extracting in n-butanol 1-3 times, recovering n-butanol to obtain extract B, passing it through polyamide column, water washing, concentrating to obtain coarse saponin, chromatography by silica gel column, eluting with chloroform-methanol-water, collecting coarse momordicoside V, chromatography, water washing, eluting with alcohol, and collecting product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for preparing high-purity mogroside V from Luo Han Guo. technical background [0002] Luo Han Guo is the dried and mature fruit of the perennial herbaceous vine Momordicagrosvenori Swingle of the cucurbitaceae plant Luo Han Guo. It is a commonly used traditional Chinese medicine. It has the effects of clearing away heat and relieving heat, moistening the lungs and quenching thirst. , whooping cough, acute and chronic bronchitis and acute and chronic tonsillitis and other diseases. At the same time, Luo Han Guo is also a commonly used dried fruit for beverages, and its extract is a commonly used sweetener, which is exported in large quantities and exported to Southeast Asia, Canada, the United States and other places. [0003] The main components in Luo Han Guo and Luo Han Guo extracts are cucurbitane-type triterpene saponins, including mogroside IV (...

Claims

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Application Information

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IPC IPC(8): C07J9/00A23L1/236A23L27/30
Inventor 李晓波王梦月屠鹏飞周兢
Owner SHANGHAI JIAO TONG UNIV
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