Method for separating preparing anthocyan monomer from mulberry
An anthocyanin and mulberry technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, and medical preparations containing active ingredients, etc., can solve the problems of large separation technology, small separation amount, difficult regeneration and use, etc. Preparation, low preparation cost, good separation effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0040] Example 1
[0041] The solvent system of this embodiment uses TBME: acetonitrile: n-butanol: TFA aqueous solution=1:1:3:5 according to the volume ratio, the concentration of the TFA aqueous solution is 1ml TFA per liter of the aqueous solution, and the countercurrent chromatograph is HSCCC-D400 High-speed countercurrent chromatograph, the anthocyanin content in the crude mulberry anthocyanin extract is about 46%.
[0042] Measure 150ml of TBME, 150ml of acetonitrile, 450ml of n-butanol and 750ml of trifluoroacetic acid aqueous solution, place them in a 2000ml separatory funnel, shake well, stand for layering, and put the upper and lower phases into reagent bottles respectively. The upper phase was injected into the column of high-speed countercurrent chromatography at a flow rate of 20ml / min. Turn on the countercurrent chromatograph to 800 revolutions / min, and then pump the sample solution (15ml lower phase solution with 0.6g of mulberry anthocyanin crude extract dissolved)...
Example Embodiment
[0043] Example 2
[0044] The solvent system of this embodiment uses TBME: acetonitrile: n-butanol: TFA aqueous solution = 2:1:2.5:6 according to the volume ratio, the concentration of the TFA aqueous solution is 5 ml TFA per liter of the aqueous solution, and the countercurrent chromatograph is HSCCC-D400 High-speed countercurrent chromatograph, the anthocyanin content in the crude mulberry anthocyanin extract is about 46%.
[0045]Measure 300ml of TBME, 150ml of acetonitrile, 375ml of n-butanol and 900ml of TFA aqueous solution, place them in a 2000ml separatory funnel, shake well, stand for layering, and put the upper and lower phases into reagent bottles respectively. The upper phase was injected into the column of high-speed countercurrent chromatography at a flow rate of 20ml / min. Turn on the countercurrent chromatograph to 800 revolutions / min, and then pump the sample solution (15ml lower phase solution with 0.6g of mulberry anthocyanin crude extract dissolved) at a flow ra...
Example Embodiment
[0046] Example 3
[0047] The solvent system of this embodiment adopts TBME:acetonitrile:n-butanol:TFA aqueous solution=1:1:3.5:6 according to the volume ratio, the concentration of TFA aqueous solution is 5ml TFA per liter of aqueous solution, and the countercurrent chromatograph is HSCCC-D1000 High-speed countercurrent chromatograph, the anthocyanin content in the crude mulberry anthocyanin extract accounts for about 38%.
[0048] Measure 250ml of TBME, 250ml of acetonitrile, 875ml of n-butanol and 1500ml of TFA aqueous solution, place them in a 3000ml separatory funnel, shake well, stand for layering, and put the upper and lower phases into reagent bottles respectively. The upper phase was injected into the column of high-speed countercurrent chromatography at a flow rate of 40ml / min. Turn on the counter-current chromatograph to 800 revolutions / min, then pump the sample solution (40ml lower phase solution with 1.5g of mulberry anthocyanin crude extract dissolved) at a flow ra...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap