Process of measuring matrine content in Qingbai cleaning lotion

Abstract

The process of measuring matrine content in Qingbai cleaning lotion includes the following steps: preparing reference substance solution of 0.01-0.1 mg / ml concentration with matrine and methanol; preparing sample solution with sample in 5-20 ml through adding concentrated ammonia solution in 0.25-1 ml, adding chloroform in 20 ml and shaking for over three times, merging the chloroform solutions, evaporating to dry, dissolving in chloroform, setting in alkali alumina column, eluting with chloroform-methanol solvent (2-10 to 0-2) in 10-50 ml, collecting and evaporating to dry the eluted solution, dissolving in methanol, transferring to a 50 ml measuring flask, diluting with methane to the scale, shaking and filtering with microporous filter film to obtain the filtrate; measuring the reference substance solution and sample solution of 5-40 microliter each in a liquid chromatograph and calculation to obtain the result. The present invention is fast and accurate.

Description

Technical field: [0001] The present invention relates to a kind of matrine (C 15 h 24 N 2 O) content assay method, particularly relate to the active ingredient in Qingbai Body Cleansing Lotion---the assay method of matrine. Background technique: [0002] Qingbai Jieshen lotion is a kind of external gynecological lotion of Chinese patent medicine, which is now included in the "National Standards Compilation of Chinese Proprietary Medicine Local Standards of Chinese Proprietary Medicines Rise to National Standard Part Surgical and Gynecological Subvolume" (National Drug Administration, 2002) No. 339 ~342 pages, the prescription consists of the following medicines: Sophora flavescens 120g, Coptidis 80g, Phellodendron 120g, Zanthoxylum bungeanum 40g, Cnidium 120g, Astragalus 120g, Polygonum multiflorum 120g, Kochia chinensis 120g, Daqingye 120g, Chishao 120g, Angelica 120g, Sodium lauryl sulfate 4g, benzoic acid 2g, the preparation method is to decoct the above eleven medicin...

AI Technical Summary

Problems solved by technology

The active ingredient in Qingbai Body Cleansing Lotion is matrine. In the current standard content determination items of Qingbai Body Cleansing Lotion, the content of matrine is determined by scanning matrine by thin layer chromatography. , due to the cumbersome operation of thin-layer chromatography, which is greatly affected by factors such as thin-layer plates, operators, and instruments, its reproducibility is poor, and it is difficult to achieve the purpose of accurately determining the content of matrine in it, resulting in Qingbaijie Poor quality controllability of body lotion