Microbe for degrading nitrite, separating and bring up method, and application

A technology of nitrite and target microorganisms, which is applied in the field of microorganisms degrading nitrite, separation, cultivation and application, and can solve problems such as nitrite pollution

Inactive Publication Date: 2007-10-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, due to the application of chemical fertilizers, sewage irrigation, garbage and feces, industrial nitrogenous waste, and nitrogenous waste gas emitted from fuel combustion in the environment, under natural conditions, nitrite is formed after leaching and decomposition by precipitation, which flows into rivers and lakes and seeps into the ground. , resulting in nitrite pollution of surface water and groundwater

Method used

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  • Microbe for degrading nitrite, separating and bring up method, and application
  • Microbe for degrading nitrite, separating and bring up method, and application
  • Microbe for degrading nitrite, separating and bring up method, and application

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Isolation of Bacillus megaterium CGMCC No.1627

[0018] Collect waste tobacco dust samples from the tobacco environment, add sodium nitrite, add 100mL sterile saline, and incubate at 30°C and 120rpm for 36h. According to the 10-fold dilution method to make 10 -1 、10 -2 、10 -3 , ... 10 -9 The bacterial liquid was streaked on the solid screening medium, the screening medium was glucose 1.5%, sodium nitrite 0.1%, dipotassium hydrogen phosphate 0.135%, potassium dihydrogen phosphate 0.07%, magnesium sulfate 0.02%, pH 7, agar 2 %. Select a single bacterium and carry out streak culture repeatedly until a pure bacterium is obtained, and store it on a peptone slant of beef extract for future use.

[0019] Eight strains that grew faster on the screening medium were selected for the nitrite degradation experiment. The medium used in the degradation experiment was: glucose 1.5%, sodium nitrite 0.015%, dipotassium hydrogen phosphate 0.135%, potassium dihydrogen phosph...

Embodiment 2

[0026] Degradation of Nitrite by Bacillus megaterium CGMCC No.1627

[0027] Bacillus megaterium CGMCC No.1627 of the present invention is inoculated in the following medium: glucose 1.5%, peptone 0.2%, sodium chloride 0.1%, dipotassium hydrogen phosphate 0.135%, potassium dihydrogen phosphate 0.07%, magnesium sulfate 0.02% , sodium nitrite 0.0138%, pH 7; the culture conditions are 30°C, 150rpm for 24h. Then the nitrite content in the culture medium was determined. Bacillus megaterium CGMCC No.1627 can degrade 99.84% of nitrite in the medium.

Embodiment 3

[0029] Nicotine tolerance test of Bacillus megaterium CGMCC No.1627

[0030] The basic medium used in the nicotine tolerance test is as follows: glucose 1.5%, peptone 0.2%, sodium chloride 0.1%, dipotassium hydrogen phosphate 0.135%, potassium dihydrogen phosphate 0.07%, magnesium sulfate 0.02%, sodium nitrite 0.0138 %, pH 7. By adding 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6% nicotine to the basic medium respectively, a series of mediums for testing the ability of Bacillus megaterium to tolerate nicotine are formed. Bacillus megaterium CGMCC No.1627 of the present invention was inoculated in the above-mentioned culture medium respectively, the inoculum size was 4%, cultivated at 30°C and 150rpm for 16h, and the OD of the fermentation broth was 600 Values ​​represent the growth of Bacillus megaterium CGMCC No.1627, and the degradation rate of nitrite in the fermentation broth was determined. The results are shown in Table 2.

[0031] Nicotine concentration (%)

[003...

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Abstract

This invention relates to a microbe that can degrade nitrite with a high efficiency, which is B. megaterium MPF-906 (CGMCC No.1627). This invention also discloses a method for separating and culturing B. megaterium MPF-906. This invention also discloses the application of B. megaterium MPF-906 in degrading nitrite. B. megaterium MPF-906 can reduce the content of nitrite in water, soil and tobacco, thus can reduce the damage of nitrite to human bodies and environment.

Description

technical field [0001] The present invention relates to a Bacillus megaterium (B.megaterium) MPF-906 capable of efficiently degrading nitrite, which has been preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) on March 3, 2006, with a preservation number It is CGMCC No.1627; the present invention also relates to the isolation method, culture method and application of Bacillus megaterium CGMCC No.1627, belonging to the field of biotechnology. Background technique [0002] Nitrite mainly refers to sodium nitrite and potassium nitrite, which are white opaque crystalline chemical products, shaped like table salt. Nitrite is a highly toxic substance. Adults can cause poisoning if they ingest 0.2-0.5 grams, and 3 grams can be fatal. Nitroso compounds are widely found in pickled, cured, and fermented foods, such as home-made pickles, bacon, salted fish, bacon, bacon, cheese, sauerkraut, as well as soy sauce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/11
Inventor 孙君社马鹏飞刘萍张京声
Owner CHINA AGRI UNIV
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