Quick detection method for agricultural chemical residue in milk

A detection method and technology for pesticide residues are applied in the field of rapid detection of pesticide residues in animal milk and dairy products, achieving the effects of simple instrument, reduced laboratory workload and simple operation.

Inactive Publication Date: 2007-10-24
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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AI Technical Summary

Problems solved by technology

The rapid pesticide residue detection technology designed based on enzyme inhibition is applied to the determination of pesticide residues in fruits and vegetables, but has not been applied to milk with a relatively complex matrix. Here, we will develop these methods and apply them to the determination of pesticide residues in milk

Method used

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  • Quick detection method for agricultural chemical residue in milk
  • Quick detection method for agricultural chemical residue in milk
  • Quick detection method for agricultural chemical residue in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Rapid detection experiment and detection limit verification of dichlorvos pesticide residues in milk

[0037] Experimental pesticide: Dichlorvos:

[0038] mouse oral LD 50 50-92mg / kg; rat oral 50-110mg / kg.

[0039] 10 concentrations were used in the experiment, and the test results were repeated three times (Fig. 1).

[0040] The obtained inhibition rates of different concentrations are listed in Table 1 below, and Figure 1 is drawn according to the obtained inhibition rates.

[0041] Experimental steps:

[0042] 1. Take the test samples of different concentrations of pesticides and acetone in an appropriate ratio, mix and shake, and let stand for 10 minutes until the supernatant appears.

[0043] 2. Take 5ml of the supernatant and place it in a colorimetric tube, blow it with nitrogen and concentrate until there is no solvent.

[0044] 3. Add 2 mL of phosphate buffer solution with a pH of 8.0 to the above colorimetric tube, then add 40 μL of cholinesterase ...

Embodiment 2

[0057] Rapid detection experiment and detection limit verification of methamidophos pesticide residues in milk

[0058] Experimental pesticide: Methamidophos:

[0059] Methamidophos acute toxicity: oral LD ​​in rats 50 7.5mg / kg; rats inhaled LC 50 9mg / kg; rat percutaneous LD 50 : 50mg / kg.

[0060] Methamidophos designed 10 concentration gradients, and the experimental results were repeated three times (Fig. 2).

[0061] Experimental steps:

[0062] 1. Take the test samples of different concentrations of pesticides and acetone in an appropriate proportion, mix and shake, and let stand for 20 minutes until the supernatant appears.

[0063] 2. Take 7.5ml of the supernatant and place it in a colorimetric tube, blow in nitrogen gas, and concentrate until there is no solvent.

[0064] 3. Add 3 mL of phosphate buffer solution with a pH of 7.0 to the above colorimetric tube, then add 50 μL of cholinesterase and 50 μL of chromogenic reagent dithio-dinitrobenzoic acid (DTN...

Embodiment 3

[0077] Rapid detection experiment and detection limit verification of monocrotophos pesticide residues in milk

[0078] Experimental pesticide: Monocrotophos:

[0079] mouse oral LD 50 5mg / kg; rat oral LD 50 21mg / kg; Rabbit percutaneous LD 50 149~709mg / kg

[0080] Using 10 concentrations, the test results were repeated three times (Fig. 3).

[0081] Experimental steps:

[0082] 1. Take the test samples of different concentrations of pesticides and acetone in an appropriate proportion, mix and shake, and let it stand for 30 minutes until the supernatant appears.

[0083] 2. Take 10ml of the supernatant and place it in a colorimetric tube, blow in nitrogen gas, and concentrate until there is no solvent.

[0084] 3. Add 4 mL of phosphate buffer solution with a pH of 7.5 to the above colorimetric tube, then add 60 μL of cholinesterase and 60 μL of chromogenic agent dithio-dinitrobenzoic acid (DTNB); A control group with the same processing steps as the sample grou...

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PUM

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Abstract

The invention relates to a quick check method of milk pesticide, as enzyme-restrain rate method, which uses colorimetry to process quick test on left pesticide, with simple operation, high automation, needed sensitivity, low cost, and wide application for testing the left pesticide in milk.

Description

Technical field: [0001] The invention relates to a rapid detection method for pesticide residues, in particular to a rapid detection method for pesticide residues in animal milk and dairy products. Background technique: [0002] Today, as food safety is increasingly concerned, strictly controlling product quality and avoiding the impact of drug residues on food has become the focus of quality control. However, as an important source of food pollution, pesticides are more toxic to the human body because most of their varieties are used to prevent and control agricultural pests. Pesticides entering the human body, if they exceed the maximum tolerance limit of normal people, will lead to the disorder of the normal physiological functions of the body, causing pathological changes and toxicity hazards. Therefore, the problem of pesticide residues in food should also be given sufficient attention. [0003] With the increasing consumption of dairy products in our country, strict c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G06F19/00
Inventor 李翠枝王彦娟刘丽君
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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