Tubule high-density type method for freezing pig jism and the products thereof

A pig semen, high-density technology, applied in the preservation of human or animal bodies, medical raw materials derived from mammals, animal husbandry, etc., can solve the restrictions on the production and application of pig frozen semen, shorten the change of capacitation, and fail to achieve the application effect To achieve the effects of stable genetic progress and strict biosafety, easy communication and transportation, and scientific quality evaluation indicators

Inactive Publication Date: 2007-10-31
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0005] Due to the ubiquitous problems such as low survival rate of pig frozen semen after thawing, lack of vigor, and capacitation-like changes that shorten the survival time, resulting in low fertilization rate and small litter size after insemination, even if the insemination dose is increased Also can not reach ideal application effect, has seriously limited the production and application of pig semen, urgently needs to establish a kind of technically advanced, reliable, stable and practical pig semen cryopreservation method

Method used

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  • Tubule high-density type method for freezing pig jism and the products thereof
  • Tubule high-density type method for freezing pig jism and the products thereof
  • Tubule high-density type method for freezing pig jism and the products thereof

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Embodiment Construction

[0033] The production and evaluation methods of pig thin tube high-density frozen semen are as follows:

[0034] 1. Pig semen collection and inspection:

[0035] The pig semen was collected by conventional methods, filtered through four layers of gauze, and various indicators were checked. The semen with motility > 0.7, deformity rate 70% was used for cryopreservation.

[0036] 2. Pretreatment of Semen

[0037] 1) Pre-equilibration of semen: Dilute the semen 1:1 (v / v) with a room temperature preservation solution (ZO solution or S solution, Table 1), and stand at room temperature (16-28°C) in the dark for 1 hour.

[0038] ZO liquid

S liquid

glucose

Sodium citrate

citric acid

cysteine

EDTA

BSA

Tris

NaHCO 2

1.15

1.17

0.41

0.01

0.23

0.50

0.65

0.125

4

0.37

0.24

[0039] 2) Centrifugation of semen: After standing still, divide the semen into 15mL...

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Abstract

The invention discloses a high density freezing maintain pig seminal fluid method with fistula and product in animal propagation technical domain, which comprises the following steps: collecting fresh seminal fluid; detecting; pre-treating the seminal fluid; defreezing; proceeding assessment procedure of defreeze seminal fluid; diluting with freezing diluting liquid; storing with high density super low temperature with 0. 25mL fistula; stabilizing the defreezing sperm above 0. 5 and average active at 0. 58; reaching the highest at 0. 68; proceeding pig external fertilization with the sperm; getting 54. 3% cleavage ratio and 48. 5% morula developing ratio; meeting one sperm dosage with sperm quantity of one 0. 25mL fistula. This invention possesses larger using latent force and market developing prospect.

Description

1. Technical field [0001] The invention relates to a method and a product thereof for high-density cryopreservation of pig semen in thin tubes, belonging to the technical field of animal reproduction. 2. Technical Background [0002] The technical difficulty of ultra-low temperature cryopreservation of pig semen is relatively difficult, which has been the main factor restricting its entry into the practical production stage for a long time. The breakthrough of this technology can not only solve the problem of long-term preservation of pig semen, but also help to promote the application of pig artificial insemination technology, which is conducive to the protection of pig germplasm resources in my country and the world, so that the application of semen is not limited by time and region , which is convenient for germplasm exchange and transportation, which can greatly increase the utilization rate of excellent breeding boars; in addition, boars’ frozen semen can be supplied in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/52A01N1/00
Inventor 芮荣高俊锋郑筱峰
Owner NANJING AGRICULTURAL UNIVERSITY
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