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Application of ophiopognin saponin D in preparing vascular endothelial cells apoptosis regulating gene medicine

A technology of Ophiopogon saponin and vascular endothelium is applied in the application field of Ophiopogon saponin D in the preparation of gene drugs for regulating vascular endothelial cell cycle, and can solve the problems of unusability, interference peaks, low sensitivity and the like

Inactive Publication Date: 2007-12-12
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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AI Technical Summary

Problems solved by technology

[0009] Because saponin is a kind of compound with large polarity and complex structure, it has no ultraviolet absorption and no specific chromogenic agent, and the determination of Ophiopogon japonicus saponin is often interfered by sugar. If the terminal absorption method of ultraviolet detection is used, the sensitivity Low, with interference peaks, unusable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Example: Study on the regulation of Ophiopogon japonicus saponin D on vascular endothelial cell apoptosis genes

[0017] The active ingredient Ophiopogon japonicus saponin D extracted from the Chinese medicinal material Ophiopogon japonicus has anti-H on human umbilical vein endothelial cells 2 O 2 The role of apoptosis. The invention uses gene chip technology to find the gene expression profile of vascular endothelial cells regulated by Ophiopogon japonicus saponin D for apoptosis-related genes.

[0018] 1Materials and methods

[0019] 1.1 Main reagents: RPMI-1640 medium (GIBCO, USA) (each liter contains 20% calf serum, L-glutamine 0.33mg, penicillin 100u, streptomycin 100u, pH-7.4); digestive agent is 0.25% Trypsin (MERCK, USA); Ophiopogon japonicus produced in Mianyang, Sichuan, purchased from Nanjing Medicinal Materials Company, and identified as Ophiopogon japonicus. (Lf) Ker-Gawl. root by the Department of Chinese Medicine Identification of Nanjing University of Tradi...

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PUM

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Abstract

The invention uses cellular apoptosis gene chip in detecting differentia expression of 120 pieces key apoptosis gene regulated and controlled by H2O2 and ophiopogonin D. Research with gene chip technology shows that H2O2 can remarkably up regulate 27 pieces gene and down regulate 3 pieces gene while ophiopogonin D performs antagonism, thereby protecting endothelial cell of blood vessel, which is importance part for preventing and treating cardio-cerebrovascular disorder.

Description

1. Technical Field [0001] The invention relates to a new application of Ophiopogon japonicus saponin D in pharmacy, and specifically relates to the application of Ophiopogon japonicus D in the preparation of gene drugs regulating the vascular endothelial cell cycle, belonging to the technical field of traditional Chinese medicine. 2. Background technology [0002] 1. Ophiopogon japonicus completes the effect of nourishing yin and promoting blood circulation by protecting vascular endothelial cells [0003] Ophiopogon is a commonly used product for clinical nourishing yin solution and one of the representative drugs for nourishing yin and promoting fluid. Ophiopogon japonicus (Ophiogon japonicus (Thumb.)) Ker-Gawl. Mainly contains a variety of glycosides: Ophiopogon japonicus saponins B, D, 23,24 dihydroxy Roskau soap, Ophiopogon aglycone-3-OaL-rhamnopyranosyl(1-2)-β-D-pyridine Glucopyranoside, Methyl Ophiopogon Flavone A, B, Ophiopogon Flavone A, B, 6-aldehyde Iso Ophiopogon A, B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P9/00C12Q1/68C07H1/08
Inventor 张旭陆兔林蔡宝昌王明艳毛春芹张小燕张志洁杨进
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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