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Method for preparing saikosaponin

A technology of saikosaponin and bupleurum, which is applied in the field of preparation of traditional Chinese medicine extracts, can solve the problems of reduced physiological activity, low extraction efficiency, and easy chemical changes, and achieve the effect of not easy to degrade, high extraction efficiency, and high content

Inactive Publication Date: 2011-09-21
TIANJIN TASLY ZHIJIAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of saikosaponin mostly adopts the method of water extraction and alcohol precipitation, the extraction efficiency is not high, chemical changes are prone to occur during extraction and separation, and the physiological activity is greatly reduced. Therefore, it is important to study more effective and stable extraction and separation methods of saikosaponin. the meaning of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take Bupleuri herb, crush it, add water to boil and extract three times and control the pH, the first time is 6 times the amount of water and 0.7% of the amount of Na2CO3, the second time is 4 times the amount of water and 0.3% of the amount of Na2CO3 , the third time is 4 times the water volume of the medicinal material; side countercurrent circulation extraction, after boiling, keep for 1.5, 1, and 1 hour respectively; the medicinal liquid is combined and cooled to room temperature, filtered, and the macroporous weakly basic anion that has been treated on the filtrate D392 exchange column (chemical factory of Nankai University), rinse with 2% NaOH solution of 2 times of resin bed volume, and discard the rinse solution; then rinse with 3 times of pure water, discard the water rinse solution; use 70% of the resin bed volume of 7 times Elute with ethanol, collect the eluent, concentrate to thick paste (relative density at 50°C is 1.28-1.33), and dry with dynamic belt dryi...

Embodiment 2

[0017] Take the residue after extracting volatile oil from Bupleuri, crush it, add water and ultrasonically extract it four times and control the pH, the first time is 5 times the amount of water and 0.9% NaHCO3 of the amount of the drug, and the second time is 4 times the amount of water and 0.5% of the amount of the drug % NaHCO of the medicinal material amount, the third and fourth times are 3 times the amount of water of the medicinal material amount; the medicinal liquid is combined and cooled to room temperature, filtered, and the treated macroporous type weakly basic anion D380 exchange column (Nankai University Chemical Plant), washed with 2.5% NaOH solution of 1.5 times the volume of the resin bed, and discarded the rinse solution; rinsed with 4 times of pure water, discarded the water rinse solution; eluted with 65% ethanol of 8 times the volume of the resin bed, and collected The eluate is concentrated to a thick paste (relative density is 1.28-1.33 at 50° C.), and d...

Embodiment 3

[0019] Take the residue after extracting volatile oil from Bupleuri, add 50% ethanol to reflux extraction three times and control the pH, the first time is 5 times the amount of 50% ethanol and 1% Na2CO3 of the amount of the medicinal material, and the second time is 4 times the amount of the medicinal material 50% ethanol and 0.5% medicinal Na2CO3, the third time is 4 times the amount of medicinal materials and 50% ethanol; side countercurrent circulation reflux extraction, after the reflux starts, keep it for 1.5, 1, 1 hour respectively; filter the medicinal liquid and combine the filtrate , after the filtrate was concentrated under reduced pressure at 70°C to no alcohol smell, the concentrated solution was diluted with water to a ratio of medicinal material to liquid of 1:1.5, filtered, and the filtrate was treated with a macroporous strong basic anion D201 exchange column (Nankai University Chemical Industry Co., Ltd. factory), washed with 2% NaOH solution 2 times the volum...

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Abstract

The invention discloses a preparation method of Chinese traditional medicine extract, specifically to a preparation method of saikosaponin. The method comprises the following steps in sequence: adding solvent to radix bupleuri or residue of radix bupleuri after extraction of volatile oil, regulating to alkality, extracting to obtain extract, loading on macroporous basic anion-exchange column, eluting with basic solution and pure water, discarding the flushing liquor, eluting with aqueous ethanol, and concentrating the eluant to obtain saikosaponin. The inventive extraction method of saikosaponin has high extraction efficiency and saikosaponin content; can keep high biological activity of saikosaponin; and saikosaponin is not easy to degrade during extraction separation.

Description

technical field [0001] The invention belongs to the preparation method of traditional Chinese medicine extract, in particular to the preparation method of saikosaponin. Background technique [0002] Bupleurum bupleurum is the dried root of Bupleurum bupleurum or Bupleurum angustifolia, which can be divided into "Northern Bupleurum" and "Southern Bupleurum". Alternating cold and heat, chest and hypochondriac abdominal pain embolism. Saikosaponin is one of the main active ingredients of Bupleurum bupleurum. It has corticosterone-like effects, inhibits the activity of Na+, K+-ATPase, has immune regulation, anti-platelet active factor function, and has obvious anti-inflammatory and antipyretic effects. , Analgesic effect, pharmacological experimental research shows that saikosaponin can make the body temperature of rabbits with fever caused by hot pricking method return to normal or lower after 1.5 hours, and then rise to normal again. At present, the extraction of saikosaponi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/233A61K9/19A61K47/40A61P29/00A61K125/00
Inventor 杨悦武叶正良郑永锋李旭
Owner TIANJIN TASLY ZHIJIAO PHARMA
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