Method for measuring density of anti-epileptic in blood

An anti-epileptic drug and blood drug concentration technology, applied in the field of medical testing, can solve the problems of tedious and time-consuming operation, expensive instruments, equipment or reagents, and high analysis cost, and achieve the effects of less plasma consumption, easy operation, and avoiding interference.

Inactive Publication Date: 2007-12-26
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method reported in the literature has various disadvantages: such as cumbersome and time-consuming operation, low efficiency, long measu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring density of anti-epileptic in blood
  • Method for measuring density of anti-epileptic in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Chromatographic conditions

[0022] Waters 2690 HPLC system, Waters 2487 dual-wavelength UV detector, Millennium 32 chromatographic workstation (Version 4.0); chromatographic column: Agilent ZORBAX Eclipse XDBXDB C 18 (150mm×4.6mm, 5μm); Column temperature: 40°C; Mobile phase: Methanol: 0.1% acetic acid solution (pH 2.3±0.2) (38:62, V / V); Flow rate: 1.5mL / min; Determination of PB at 215nm And the concentration of MHD, 240nm to measure the concentration of LMG and OXC.

[0023] Plasma sample pretreatment

[0024] Precisely draw 100μL of plasma into a 1.5mL Eppendorf tube, add 20μg·mL -1 Chlorzoxazone methanol working solution 300μL, vortex for 10s, centrifuge (12000×g, 4°C) for 10min, take 20μL of the supernatant and inject. The internal standard method was quantified by peak area.

[0025] selectivity

[0026] Blood samples from 10 healthy volunteers from different sources and 20 blood samples from epilepsy patients who did not take PB, LTG, OXC and MHD were taken,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for determining blood-medicine concentration of antiepileptic medicine includes pre-treating sample to be determined and separating pretreated sample by acidic mobile phase in chromatographic column then utilizing ultraviolet detector to simultaneously detect quantitative concentration of phenobarbital, lamotrigine, oxcarbazepin and adipomono- oxcarbazepin as antiepileptic medicine.

Description

technical field [0001] The invention belongs to the field of medical examination, and relates to an analysis and determination method of drugs in vivo, in particular to a method for measuring blood drug concentration of antiepileptic drugs. Background technique [0002] Phenobarbital (PB), lamotrigine (LTG), oxcarbazepine (OXC) are commonly used antiepileptic drugs in clinic. Among them, oxcarbazepine OXC can be rapidly metabolized into the active metabolite monohydroxycarbazepine (Monohydroxycarbazepine, MHD) in vivo. Because the pharmacokinetics of these drugs vary greatly among individuals, the therapeutic window is narrow, and the pharmacokinetic characteristics, antiepileptic effect and adverse drug reactions are related, so it is of great clinical significance to carry out therapeutic drug monitoring of the above drugs. [0003] At present, there are mainly high-performance liquid chromatography (HPLC) and immunoassay methods for measuring the plasma concentration of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/60G01N30/50G01N21/31
Inventor 焦正施孝金
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap