Curtain-shape anchoring culture bioreactor and method for culturing plant cell, tissue or organ using the same

Abstract

A curtain-type anchoring biological culturing reactor and its method for culturing vegetable cell, tissue or organs are disclosed. The reactor consists of bed(E), curtain-type netted double-layer anchoring culturing bed(A), supply system(B), hanger(C), composite circulation feeder(F) and disposable sterile sealing film(G). (A) is hung on (C) and has (E), (F) is arranged on top of (E), (E) is sleeved with (G). It's simple, safe and cheap. It has less consumption and can be used for continuous production.

Description

technical field [0001] The invention relates to a bioreactor and a method for cultivating plant cells, tissues or organs in large quantities using the bioreactor, which is suitable for the cultivation of microorganisms, plants, and animal cells, tissues or organs, and belongs to the plant cell cultivation project of biotechnology field, or according to the scope of application, it belongs to the interdisciplinary field of biotechnology and chemical engineering and technology - the field of bioindustrialized production. Background technique [0002] In order to realize the large-scale cultivation of plant cells, tissues and organs for industrial production of useful secondary metabolites, since Routies and Nichell applied for the first patent in 1956 to use bubble column reactors to cultivate plant cells to produce useful secondary metabolites, worldwide Research and development of mechanically stirred STR reactors, including spirally stirred STR with spiral impeller, lift st...

AI Technical Summary

Problems solved by technology

Since the cultivation cycle of plants is much longer than that of microorganisms, in the long-term continuous cultivation process, they have to face the continuous maintenance of the excellent genetic characteristics and physiological and biochemical characteristics of plant cells, tissues and organs, the consistent control of cell growth, and the indiscriminate cultivation process. Bacterial conditions and various physical and chemical factors, various uncertainties caused by cell growth and agglomeration, gas-liquid, liquid-solid, and energy mass transfer characteristics are difficult to solve, and it is difficult to scale up industrially.

Excessive investment costs and excessive operating costs caused by too many and too long non-production processes, coupled with too many uncertainties in control, bring too high and unaffordable commercial costs to industrialized mass production. risk, seriously hindering the realization of its large-scale commercial production

In particular, step-by-step amplification multiplies the cost and risk. In the end, as long as one failure occurs, the company may go bankrupt.

It takes a long time to cultivate plant cells, tissues and organs, and there are too many variables in the process, so it is very difficult to control sterility alone

Therefore, if the cost of one-time investment is too concentrated, the commercial investment risk of large-scale cultivation of industrial production will be extremely huge, and no enterprise can bear it

[0003] In addition, the plant cells, including tissues and organs, have been reported to produce useful secondary metabolites in large quantities. Regardless of the methods, approaches, means, etc., the final results without exception make plants from complex to simple. Explants with complex structures dedifferentiate into callus, from high-level to low-level or from relatively advanced to relatively primitive, that is, from higher plants with various organ differentiations to lower or primitive cell clusters, and even Single cell, from sexual reproduction to asexual reproduction, from autotrophic to heterotrophic, from terrestrial to aquatic, from terrestrial plants to liquid suspension culture cells or cell clusters

That is to say, the entire process of mass cultivation of cells, tissues, and organs currently implemented on plants may actually be a process of reverse evolution domestication and cultivation. People attempt to cultivate and domesticate plants to be like microorganisms, which can be produced by fermentation. As a result, it is ignored that this process not only changes the shape of the plant, but more likely changes the physiological and biochemical characteristics and metabolic functions of the plant.

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