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Metabolic syndrome-improving agent and medicine, supplement, functional food and food additive containing the same

一种代谢综合症、改善剂的技术，应用在功能性食品和食品添加剂领域，能够解决没有组织特异性等问题

Inactive Publication Date: 2008-01-16
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PPARδ has no tissue specificity and is expressed throughout the body, but it is significantly expressed in colorectal cancer cells

Method used

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  • Metabolic syndrome-improving agent and medicine, supplement, functional food and food additive containing the same
  • Metabolic syndrome-improving agent and medicine, supplement, functional food and food additive containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] As described below, this example is an example of confirming that bergamot can activate PPARγ.

[0034] Inoculate CV-1 cells (cultured cells from male African green monkey kidney) to 0.2 μg / well in a 24-well culture plate at 37°C, 5% CO 2 Conditioned for 24 hours. As a medium, DMEM (Dulbecco's Modified Eagle Medium: GIBCO) containing 10% FBS (fetal bovine serum) and 10 mg / mL penicillin-streptomycin solution was used. pM-hPPARγ and p4×UASg-tk-luc were transfected using Lipofectamine system (trade name, Invitrogen). In addition, the above-mentioned pM-hPPARγ is a fusion protein expression plasmid combining the GAL4 gene (amino acid sequence 1-147) and the human PPARγ ligand-binding site gene (amino acid sequence 204-505), and p4×UASg-tk-luc is a fluorescent A reporter plasmid obtained by inserting the GAL4 response sequence (UAS) four times upstream of the ribase gene. About 24 hours after transfection, samples of bergamot at various concentrations (0.1, 1.0, 10, 50 an...

Embodiment 2

[0041] As described below, this example is an example of confirming that bergamot can activate PPARα.

[0042] Except for using pM-hPPARα instead of pM-hPPARγ, and setting the concentration of bergamot at 0.1, 1.0, 5.0, 10, 20, 50, and 80 μM, the bergatin was measured in the same manner as in Example 1 PPARα ligand activity. The results are shown in Table 2 below and the graph in FIG. 2 .

[0043] Table 2

[0044] Add concentration

PPARα ligand activity

No treatment control (DMSO)

(0.01％)

100

Bergamot

0.1μM

95±12.9

1.0μM

92±8.0

5.0μM

118±6.1

10μM

194±15.0

20μM

350±25.5

50μM

622±79.6

80μM

558±39.4

[0045] (mean ± standard deviation)

[0046] As can be seen from Table 2 and Figure 2 above, the activity of PPARα is increased by bergamotine, and its activity is enhanced with the increase of the concentration of bergamotine. ...

Embodiment 3

[0048] As described below, this example is an example of confirming the secretion promotion of adiponectin by bergamotine.

[0049] (differentiation induction of preadipocytes)

[0050] First, prepare the following 2 types of media.

[0051] Differentiation induction medium (0.25μM: DEX, 0.5mM: MIX, 10μg / mL: insulin / 10%FBS / DMEM)

[0052] 55 mL of FBS (fetal bovine serum (manufactured by GIBCO)) was added to 500 mL of DMEM (manufactured by SIGMA) to prepare 10% FBS / DMEM. 138.75 µL of 1 mM DEX (dexamethasone) / DMSO (manufactured by Nacalai Tesque), 555 µL of 10 mg / mL insulin / PBS (manufactured by SIGMA) were added thereto. In the case of insulin / PBS, 1N HCl was previously added to PBS to make it acidic enough to dissolve insulin, and then the insulin was dissolved. MIX (3-isobutyl-1-methylxanthine) (manufactured by Nacalai Tesque) was added to a necessary amount of the above medium to make it 0.5 mM just before use, thereby preparing a differentiation induction medium. Sin...

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Abstract

A metabolic syndrome-improving agent that does not have a side effect problem and can be taken for a long period of time is provided. Bergamottin is used as the metabolic syndrome-improving agent. Because bergamottin has a PPAR a and activating action, an adiponectin secretion promoting action in an adipocyte and a VLDL synthesis inhibiting action in a liver cell, the prevention, treatment or thelike of a disease such as insulin resistance, hyperinsulinemia, type 2 diabetes mellitus, obesity, visceral fat accumulation, hypertension, hyperlipidemia or arteriosclerosis is possible, whereby theprevention, treatment or the like of metabolic syndrome is possible. Because citrus that contains bergamottin such as grapefruit has been eaten for a long time, there is no problem concerning safety,and in addition, it is low in calories, therefore it can be taken for a long period of time. Because bergamottin is tasteless and odorless, even if it is added to food, it does not destroy the specific flavor of the food. Therefore, it can be taken by adding it to food.

Description

technical field [0001] The present invention relates to an agent for improving metabolic syndrome and medicines, supplements, functional foods and food additives containing the agent. Background technique [0002] Since several years ago, the concept of metabolic syndrome has been mentioned in the US National Cholesterol Education Program (NCEP), represented by WHO (World Health Organization). Metabolic syndrome is a collection of various diseases that cause arteriosclerosis such as visceral fat type obesity, insulin resistance, diabetes, hyperlipidemia, hypertension, etc., and is in a state that is prone to angina pectoris and myocardial infarction, and is considered to be a It is caused by accumulation of visceral fat and hypertrophy of fat cells. [0003] The following two mechanisms are known as the mechanism by which the accumulation of visceral fat is associated with the onset of diabetes, hyperlipidemia, hypertension, and the like. One mechanism is that glyceride ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61K36/75A61P3/04A61P3/06A61P3/10A61P9/10A61P9/12A23L1/30C07D493/04
CPCA61K36/752A61K31/37A23L1/3002A23L33/105A61P3/00A61P3/10A61P3/04A61P3/06A61P3/08A61P43/00A61P5/48A61P9/10A61P9/12
Inventor 佐佐木贵生矢野昌充
Owner ARKRAY INC
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