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Application of yunnan fusidic acid in preparation of biological pesticide

A technology of yunnanmycin and biopesticides, which is applied in the field of biopesticide preparation, can solve the problems of excessive pesticide residues in tea and sharp reduction of tea leaves, etc.

Inactive Publication Date: 2008-01-23
云南天丰农药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to excessive pesticide residues in tea leaves, the amount of tea exported to Europe has dropped sharply

Method used

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  • Application of yunnan fusidic acid in preparation of biological pesticide
  • Application of yunnan fusidic acid in preparation of biological pesticide
  • Application of yunnan fusidic acid in preparation of biological pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0195] The preparation of Yunnanmycin adopts the steps in the following order:

[0196] A: Dissolution of medium ingredients: the solid raw material is weighed in proportion and added to the batching tank, and water is added to stir evenly, and then pumped into the fermentation tank.

[0197] B: Empty tank disinfection: that is, to sterilize the empty tank first. Pass the steam into the fermenter, raise the temperature to 121-123°C, and keep it for 25-30 minutes.

[0198] C: Practical elimination: After pouring the medium into the tank, add water to make up the volume, stir evenly, and then put steam into the jacket or coil to preheat the medium. When the temperature of the tank rises to about 95°C, Directly pass steam into the tank, heat the culture medium to 121-123°C, and maintain it for 25-30 minutes. Then use cooling water to lower the temperature to the culture temperature, and at the same time pass sterile air into the tank to keep the pressure.

[0199] D: Strains: ...

Embodiment 2

[0208]Get 10 liters of fermentation liquefaction, add diatoms to filter, discard mycelia, and the filtrate is passed through a 200-gram activated carbon column (wetted with water, column 5.5 × 55cm, for adsorption, eluted with 50% acetone, and a portion is collected for every 250 milliliters. 1-4 parts of the active parts were combined, acetone was removed, the aqueous solution was about 500 ml, adjusted to pH 3.0, passed through a 50 ml ammonium-type cation exchange column, washed with a small amount of water, and eluted with 1N ammonia water, and one part was collected per 100 ml. Combine 1-5 active parts, remove ammonia, freeze-dry the aqueous solution, and decolorize all the lyophilized products through a 100 ml column of neutral alumina (wet packing in methanol), 75% methanol, flow rate 1 ml / min, detect UV268nm, retention time 8.3 minutes). Take 1 g of the 95% pure lyophilized product, add 40 ml of concentrated hydrochloric acid, hydrolyze at room temperature for 60 hours...

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PUM

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Abstract

The invention discloses a new purpose of yunnannmycin serving as biopesticide. The research result of the invention indicates that: the yunnannmycin has good effects for preventing and curing tomato virus disease caused by cucumber mosaic virus, common tobacco mosaic virus, tobacco potato virus X, tobacco potato virus Y and TEV, tomato virus disease caused by tobacco vein-distorting virus and virus disease, mosaic disease, fern leaf, little-leaf disease, spot disease, streak disease, malformation, dwarf virus disease, sickly yellow flower and putrescence, etc. of tomatos, capsicum and Chinese cabbages caused by cucumber mosaic virus and common tobacco mosaic virus, etc. The yunnannmycin is the biopesticide with good developing and applying prospects.

Description

technical field [0001] The present invention relates to the use of yunnanmycin, in particular to the use in the preparation of biological pesticides. Background technique [0002] my country is a large agricultural country. Every year, more than 15 billion catties of grain and more than 300 million kilograms of cotton are lost due to crop diseases and insect pests. The use of chemical pesticides plays an effective role in ensuring the normal production of crops, but with long-term dependence and extensive use of chemically synthesized chemical pesticides, it has caused serious damage to the ecological environment, such as soil structure damage, soil activity reduction, and serious soil and water pollution. The biological balance of the soil environment, water body environment and the entire ecological environment has been broken; the toxic pesticide residues in almost all foods such as grains, vegetables, fruits, traditional Chinese medicine, and tea have seriously exceeded ...

Claims

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Application Information

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IPC IPC(8): A01N43/54A01P1/00
Inventor 朱英杰何永斌徐滇闽白一彬李朝荣张春华
Owner 云南天丰农药有限公司
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