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Seashell monitoring method for fresh water environment heavy metal pollution

A technology for heavy metal and mercury pollution, which is applied in general water supply conservation, water testing, material inspection, etc., to achieve the effect of obvious warning effect, wide coverage of data, and small systematic error.

Inactive Publication Date: 2008-02-13
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, except for some exploratory studies in our laboratory, there is no report on the monitoring of shellfish polluted by persistent pollutants in the freshwater environment at home and abroad. Therefore, the selection of mussel species, the establishment of monitoring methods and the reliability test of monitoring results are important. The key to the invention

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for monitoring shellfish polluted by heavy metal pollutant mercury in the freshwater environment of the present invention adopts the following process steps:

[0021] Anodonta dorsalis (36 individuals) were collected from nine sampling points around Taihu Lake during 2003-2004: Lihu Hotel, Shitang, and Dushan waters in Wuxi Wuli Lake; Wuxi Wuli Lake Venice Garden in Wuxi, Wuxi Sanshan Island, Changzhou Xueyan, Yixing Dapu, Zhejiang Huzhou and Wuxi Huazhuang waters. The live mussels were temporarily raised in aerated water for 3 days to empty the intestinal debris, and then the biological properties such as shell length, shell height, shell width, and shell weight were measured. Then the soft tissue was dissected to obtain the soft tissue, which was stored in a -20°C refrigerator after weighing.

[0022] After thawing, rinse with ultrapure water (Milliprore-Simplicity personal ultrapure water system, Milliprore-Simplicity, USA, outlet water resistivity 18.2MΩ.c...

Embodiment 2

[0028] A method for monitoring shellfish polluted by heavy metal pollutant mercury in the freshwater environment of the present invention adopts the following process steps:

[0029] Anodonta dorsalis (36 individuals) were collected from nine sampling points around Taihu Lake during 2003-2004: Lihu Hotel, Shitang, and Dushan waters in Wuxi Wuli Lake; Wuxi Wuli Lake Venice Garden in Wuxi, Wuxi Sanshan Island, Changzhou Xueyan, Yixing Dapu, Zhejiang Huzhou and Wuxi Huazhuang waters. The live mussels were temporarily raised in aerated water for 3 days to empty the intestinal debris, and then the biological properties such as shell length, shell height, shell width, and shell weight were measured. Then the soft tissue was dissected to obtain the soft tissue, which was stored in a -18°C refrigerator after weighing. After thawing, rinse with ultrapure water (Millipro, USA, Milliprore-Simplicity personal ultrapure water system, outlet water resistivity 18.2MΩ.cm) water 6 times, dry ...

Embodiment 3

[0033] A method for monitoring shellfish polluted by heavy metal pollutants in a freshwater environment of the present invention adopts the following process steps:

[0034] Anodonta dorsalis (36 individuals) were collected from nine sampling points around Taihu Lake during 2003-2004: Lihu Hotel, Shitang, and Dushan waters in Wuxi Wuli Lake; Wuxi Wuli Lake Venice Garden in Wuxi, Wuxi Sanshan Island, Changzhou Xueyan, Yixing Dapu, Zhejiang Huzhou and Wuxi Huazhuang waters. The live mussels were temporarily raised in aerated water for 4 days to empty the intestinal debris, and then the biological properties such as shell length, shell height, shell width, and shell weight were measured. Then the soft tissues were dissected and weighed and stored in a -19°C refrigerator for use. After thawing, rinse with ultrapure water (Millipro, USA, Milliprore-Simplicity personal ultrapure water system, outlet water resistivity 18.2MΩ.cm) water 9 times, dry in a drying oven at 70°C for 16h to...

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Abstract

The invention relates to a shellfish monitoring method of freshwater environment heavy metal pollutant mercury pollution, which belongs to the biological monitoring environment technology field. The invention is characterized in that living heudes are selected to be raised in aerated water. Impurities in a shell and a digestive tract are eliminated. Then, the heudes are dissected and soft tissuesof the heudes are obtained and preserved in a refrigerator. The soft tissues are fetched out, thawed, washed with water, put in a drying box and dried to be of constant weight. Then, a anodonta woodiana living heude soft tissue dry sample is grinded to be powder. Together with activated alumina, sodium carbonate and calcium hydroxide, the power is made into a specimen. A mercury analyzer is used to detect the content of the heavy metal pollutant mercury in the heude. The invention has the advantages of convenient sampling and simple pretreatment method. The accumulation characteristics of theheavy metal mercury in the heude can be precisely detected. Concentration, components characteristics, pollution degree, biological toxicity effects and differences between waters of various pollutants can be rapidly known. The invention has the advantages of good stability, high reproducibility, high comparability and small system error. Harm risks on fishery waters and development trends of allpollutants can be precisely assessed and predicated.

Description

technical field [0001] The invention relates to a method for monitoring shellfish polluted by heavy metal mercury in a freshwater environment, which can systematically and quickly monitor the pollution degree of persistent pollutant mercury in the water environment, and belongs to the technical field of biological monitoring environment. Background technique [0002] In recent years, the pollution of heavy metals and other trace elements in the water environment has restricted the rapid development of my country's economy, and the tendency to threaten people's health and quality of life has become increasingly obvious, and some have even caused major disasters. Inland fishery is a very important part of my country's fishery industry. The ecological environment of fishery waters is the foundation of fishery development. Changes in the quantity of inland fishery resources and the healthy development of aquaculture are all closely related to environmental conditions. With the...

Claims

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Application Information

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IPC IPC(8): G01N33/18
CPCY02A20/20
Inventor 杨健刘洪波
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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