Tsiklomitsin molecular engram polyalcohol and uses of the same

A molecular imprinting and tetracycline technology, applied in the direction of material separation, analysis materials, instruments, etc., can solve the problem of high price and achieve the effect of high purification efficiency

Inactive Publication Date: 2008-02-27
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Oasis HLB solid-phase extraction column is a product of WATERS company, the p

Method used

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  • Tsiklomitsin molecular engram polyalcohol and uses of the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 2.0 g of template tetracycline hydrochloride molecule (China National Institute for Pharmaceutical and Biological Control), 4.0 g of functional monomer methacrylic acid (MAA) (analytical purity, Tianjin Komiou Chemical Reagent Center), and cross-linking agent ethylene glycol dimethacrylate (EDMA) 60.0g (Fluka Company), plus 0.20g initiator azobisisobutyronitrile, dissolve and mix the above reaction mixture in a 50KHz ultrasonic cleaning machine, and add it to the reactor for in-situ polymerization. The temperature is controlled between 75 and 80°C, and the reaction time is 20 hours, to obtain a hard, bulky yellow polymer.

[0020] After the polymerization reaction, the synthesized tetracycline molecularly imprinted polymer was taken out of the reactor, ground and passed through a 100-mesh sieve. The obtained powder was extracted with 80% methanol reflux to remove the template tetracycline hydrochloride molecule (80% determined by liquid chromatography). It takes about ...

Embodiment 2

[0023] Take the template tetracycline hydrochloride molecule 4.0g (analytical grade, China National Institute for Pharmaceutical and Biological Control), functional monomer methacrylic acid (MAA) 6.0g (analytical grade, Tianjin Komiou Chemical Reagent Center), cross-linking agent dimethacrylic acid B EDMA 80.0g (Fluka Company), plus 0.4g initiator azobisisobutyronitrile, dissolve and mix the above reaction mixture in a 50KHz ultrasonic cleaning machine, and add it to the reactor at 366nm UV The in-situ polymerization was carried out under the light lamp irradiation, and the reaction time was 24 hours to obtain a hard and bulky yellow polymer.

[0024] After the polymerization reaction, the synthesized tetracycline molecularly imprinted polymer was taken out of the reactor, ground and passed through a 100-mesh sieve. The obtained powder was extracted with 80% methanol reflux to remove the template tetracycline hydrochloride molecule (80% determined by liquid chromatography). It tak...

Embodiment 3

[0027] The tetracycline molecularly imprinted solid phase extraction column obtained in Examples 1 and 2 was used to perform sample pretreatment on tetracycline and oxytetracycline spiked pork as follows:

[0028] 1. Take 5.0g of minced tetracycline and oxytetracycline spiked pork (0.1mg / kg each of tetracycline and oxytetracycline), homogenize it with 20mL0.01mol / L EDTA-2Na, add 2mL1∶1(V:V ) Aqueous trichloroacetic acid solution, centrifuged at 20000 rpm for 10 minutes, and then take the supernatant, which is the sample to be tested.

[0029] 2. Take a tetracycline molecularly imprinted solid phase extraction column (200mg / 6mL), first use 5mL methanol, 5mL water, 5mL0.01mol / LEDTA-2Na solution to pass through the column activation column, then pass all the above test samples through the column, and then use 5mL Wash with water, vacuum dry the water in the column, and finally collect with 5mL methanol. The methanol phase is dried under nitrogen at 50℃, and dissolved by adding 1mL0.0...

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Abstract

The invention discloses an abricycline molecule stamp polymer, which is characterized by the following: mixing mold fermentmycin molecule, functional monomer methacrylic acid, cross linker dimethyl acroleic acid glycol ester and trigger azobisisobutyronitrile with mass ratio at 1-5 : 2-10 : 80 : 0. 1-0. 5; polymerizing at original position; possessing 'memory' function for the spatial structure of the abricycline mold molecule; possessing high selective separating enriched property for abricycline and oxytetracycline in residue analysis sample solution; filling the product into small column of polypropene shell; getting abricycline molecule stamp solid phase extraction column.

Description

(1) Technical field [0001] The invention relates to a tetracycline molecularly imprinted polymer and its application in the determination of tetracycline antibiotic residues in animal foods. (2) Background technology [0002] Tetracycline antibiotics including oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DOXY) are widely used in the breeding of food animals and marine organisms. Tetracycline antibiotics are added to animal feed to promote animal growth, prevent or treat certain diseases, which will inevitably result in the presence of tetracycline antibiotic residues in their tissues. The Codex Alimentarius Commission and countries around the world treat tetracycline antibiotics in poultry meat. Residues have strict limit requirements, and international trade also has strict limits on their limits. [0003] At present, the methods for determining tetracycline antibiotics in food mainly include microbiological method, liquid chromatography an...

Claims

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Application Information

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IPC IPC(8): C08F20/06C08F4/04C08F2/44G01N30/00
Inventor 吴平谷
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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