Fast-culturing susceptibility and micro-detection method for mycobacteria and device thereof

Abstract

The present invention relates to a micro detection method capable of quickly culturing mycobacterium to make drug sensitivity test and drug identificiation. Said method includes the following steps: (1), treating speciment, inoculating said treated specimen in culture medium, making culture, taking out the grown acid-fast mycobacterium from biological safety cabinet and preparing bacterial suspension with defined concentration; (2), in a biological safety cabinet opening upper cover of special-purpose micro culture plate, every well interior of said culture plate has previously-added culture medium and correspondent drug, adding 0.1ml of bacterial suspension with defined concentration into every well, closing upper cover and sealing; (3), placing every plate into a sealed wet box, and placing said sealed wet box into a constant temperature incubator to make culture; and (4), taking out culture plate, utilizing optical amplification system to collect bacterial growth image and comparing said bacterial growth image with negative and positive contrast well images, if said bacterial growth image is similar to the positive contrast well image, it is drug resistance, otherwise it is sensitivity.

Description

technical field [0001] The invention relates to a method and a special device for cultivating and medically examining microorganisms. Especially mycobacteria rapid culture drug susceptibility and identification trace detection method and device. Background technique [0002] The fourth national tuberculosis epidemiological sampling survey, the results of the population-wide infection rate survey at 59 points: the experimental population was 365,097, the prevalence rate of active tuberculosis was 367 / 100,000, and the prevalence rate of positive tuberculosis was 160 / 10 million, the national tuberculosis epidemic is still very serious. Among the 27 key infectious diseases announced by the Ministry of Health, the incidence of tuberculosis ranks second and the number of deaths ranks third, both ranking ahead of SARS. [0003] Laboratory examination of tuberculosis is the main means of clinical diagnosis and epidemiological investigation of tuberculosis, among which bacteriologi...

AI Technical Summary

Problems solved by technology

However, mycobacteriophages are specific for infecting mycobacteria and can only be used for Mycobacterium tuberculosis, and are not sensitive to other tuberculosis or non-tuberculosis acid-fast mycobacteria, and cannot be detected by this method

In addition, the identification of drugs is very limited, and most antibacterial drugs cannot be detected. At present, only rifampin can be used, and the price is expensive. The reagent price of each test is 70 yuan. Even if the types of drug sensitivity are expanded in the future, if 10 commonly used The cost of reagents alone is 700 yuan, so this method currently lacks practical value

Some of the above methods are not fast enough, such as the 3D method, and the drug sensitivity time takes an average of 12 days

Although some methods are as fast as the phage method, they cannot enter the stage of clinical practice due to too few drugs that can be produced.

All these methods are very expensive and cannot be afforded by most people in the country, especially in the poor western areas where tuberculosis is highly prevalent, so they cannot be promoted at the grassroots level

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