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Method for detecting micro-trace amount methyl-phosphoric acid dimethyl ester

A dimethyl methyl phosphate, micro-trace technology, applied in the direction of color/spectral property measurement, etc., can solve the problems of complicated immobilization process, increase detection cost, inconvenience of popularization and use, etc., to facilitate popularization and use and reduce detection cost. , The effect of easy on-site inspection

Inactive Publication Date: 2008-02-27
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has some advantages of the spectral sensor detection method, it is inconvenient and expensive to obtain materials due to the animal esterase acetylcholinesterase, which increases the detection cost.
In addition, sensitive materials need to be immobilized on glass slides before detection. This immobilization process is complicated and requires professionals to operate, which is not convenient for popularization and use.

Method used

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  • Method for detecting micro-trace amount methyl-phosphoric acid dimethyl ester

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Effect test

Embodiment 1

[0029] A method for detecting trace amounts of dimethyl methyl phosphate, its specific method steps are as follows:

[0030] (1) Preparation of esterase solution

[0031] Using commercially available flour as raw material, according to the weight ratio of flour:distilled water as 1:5, add distilled water to the flour, stir for 30min, then centrifuge for 12min at a centrifugal speed of 4000r / min, collect the supernatant, and statically After leaving it for 12 hours, the plant esterase enzyme liquid is prepared.

[0032] (2) Preparation of TPPS solution

[0033] In the absolute ethanol solution, add sulfonic acid tetraphenylporphyrin (TPPS) solid powder, stir and fully dissolve, and prepare a TPPS solution with a concentration of 84.23 μmol / L.

[0034] (3) to detect

[0035] ① After steps (1) and (2) are completed, add the plant esterase enzyme solution prepared in step (1) and the TPPS prepared in step (2) to the reaction tank of the liquid phase spectrometry detection devic...

Embodiment 2

[0041] A method for detecting trace amounts of dimethyl methyl phosphate, its specific method steps are as follows:

[0042] (1) Preparation of esterase solution

[0043] Using commercially available flour as raw material, add distilled water to the flour at a weight ratio of 1:4 according to the weight ratio of flour: distilled water, stir for 60 minutes, then centrifuge at a centrifugal speed of 6000r / min for 10 minutes, collect the supernatant, and statically After leaving it for 24 hours, the plant esterase enzyme liquid is prepared.

[0044] (2) Preparation of TPPS solution

[0045] In the absolute ethanol solution, add sulfonic tetraphenylporphyrin (TPPS) solid powder, stir and fully dissolve, and prepare a TPPS solution with a concentration of 30.08 μmol / L.

[0046] (3) to detect

[0047] ① After steps (1) and (2) are completed, add the plant esterase enzyme solution prepared in step (1) and the TPPS prepared in step (2) to the reaction tank of the liquid phase spect...

Embodiment 3

[0053] A method for detecting trace amounts of dimethyl methyl phosphate, its specific method steps are as follows:

[0054] (1) Preparation of esterase solution

[0055] Using commercially available flour as raw material, according to the weight ratio of flour:distilled water as 1:6, add distilled water to the flour, stir for 10min, then centrifuge for 15min at a centrifugal speed of 3000r / min, collect the supernatant, and statically After leaving it for 8 hours, the plant esterase enzyme liquid is prepared.

[0056] (2) Preparation of TPPS solution

[0057] In the absolute ethanol solution, add sulfonic tetraphenylporphyrin (TPPS) solid powder, stir and fully dissolve, and prepare a TPPS solution with a concentration of 111.3 μmol / L.

[0058] (3) to detect

[0059] ① After steps (1) and (2) are completed, add the plant esterase enzyme solution prepared in step (1) and the TPPS prepared in step (2) to the reaction tank of the liquid phase spectrometry detection device Sol...

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Abstract

A method for trace detection of dimethyl methyl phosphate, relates to method using spectral sensor to detect organophosphor substances. The invention takes market flour, sulfo-tetraphenylporphyrin (TPPS) as raw materials; prepare the plant enzyme solution, TPPS solution, then first add TPPS solution, plant enzyme solution and phosphate buffer solution into the reaction tank of the liquid phase spectral detection device to form the TPPS-plant esterase complex solution, then add the measured sample liquid, measure and record the absorption spectrum before and after adding the sample liquid, whether the sample contains DMMP can be judged by comprising the differences of the two absorption spectrum. As the method by this invention has the characteristics of low detection cost, simple to operate, can effectively real-time detect on-site, detection limit can be as low as 10 ppt and so on, therefore, the invention can be widely used for trace residue detection of dimethyl methyl phosphate in vegetables, fruits and other agricultural products and environment.

Description

technical field [0001] The invention belongs to the technical field of organophosphorus substance detection, in particular to a method for detecting organophosphorus substances with a spectral sensor. Background technique [0002] Dimethyl methyl phosphate (DMMP) is an organophosphorus pesticide, which is currently the most widely used pesticide type. While the use of pesticides has brought huge economic benefits to people, it has also produced many negative effects. Slight pesticide residues can cause a variety of chronic diseases, and severe pesticide residues can cause acute poisoning of humans and animals, directly endangering human life. In addition, excessive pesticide residues will also seriously affect the reputation and export benefits of my country's agricultural products, making it impossible to take advantage of the competition in the international market. Due to the lag of our detection methods and means, the food and agricultural sideline products exported by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/27
Inventor 侯长军霍丹群杨丽敏
Owner CHONGQING UNIV
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