Dachengqi tang animal blood plasma freeze-dried powder and method for preparing the same

A technology of Dachengqi soup and freeze-dried powder, which is applied in freeze-dried transportation, medical raw materials derived from mammals, biological testing, etc., can solve the problems of limiting continuous progress, stability, and uniformity, and improve the reliability of experiments The effect of high speed, convenient blood collection and easy feeding

Inactive Publication Date: 2008-04-02
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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Problems solved by technology

This limits the continuous progress, st

Method used

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  • Dachengqi tang animal blood plasma freeze-dried powder and method for preparing the same
  • Dachengqi tang animal blood plasma freeze-dried powder and method for preparing the same
  • Dachengqi tang animal blood plasma freeze-dried powder and method for preparing the same

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Embodiment Construction

[0034] This example further illustrates the preparation method of the Dachengqi Decoction animal plasma freeze-dried powder of the present invention, and this example does not limit the content of the present invention in any way.

[0035] 1. Detection method

[0036] If there is no special instruction, in the present invention, active ingredient, emodin and magnolol are detected by RP-HPLC in the drug-containing plasma sample, and the detection conditions are:

[0037] Testing equipment: Waters high performance liquid chromatography

[0038] Column: Nava-pak C 18 60A, 4, 3.9×150mm

[0039] Detection wavelength: detection wavelength 294nm

[0040] Column temperature: 30°C

[0041] Injection volume: 20μl

[0042] Flow rate: flow rate 1ml / min

[0043]Mobile phase: acetonitrile: water (0.125% acetic acid, 0.4% sodium acetate) 60:40

[0044] Under this condition, the t of emodin and magnolol R They were 3.829min and 4.861min respectively, and the separation of the two and...

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Abstract

The invention provides a potent purgative decoction animal plasma freeze-dried powder and the preparation method. The preparation method includes the following procedures: (1) rats are gavaged with the medical solution potent purgative decoction for three days; (2) 30 to 45 minutes after the last feeding, the rat blood is drawn by artery puncture, the heparin is anticoagulated, the plasma is isolated, and is divided evenly and stored in clean and aseptic vessels; (3) pre-frozen for 30 minutes under conditions of -20 degrees centigrade and frozen rapidly under conditions of -80 degrees centigrade; (4)the drug-containing plasma is frozen for 2 hours, frozen and dried to produce freeze-dried powder. The preparation method solves the equivalent adding problems such as the isolated storage of the effective components that interfere with the in vitro pharmacological experiment, further improves the consistency of in vitro traditional Chinese medicine pharmacological experiment and in vivo mechanism of practical drug action, and enhances the experimental reliability.

Description

technical field [0001] The invention belongs to the technical field of blood products, in particular to a preparation method of blood products containing medicines. Background technique [0002] The traditional Chinese medicine Dachengqi Decoction is a representative prescription of Tongli Gongxia method, which has significant curative effect on peritonitis and gastrointestinal motility dysfunction. Previous scholars have done a lot of research on it, but they either stayed at the overall efficacy observation of animal models in vivo experiments, or carried out in vitro experiments with the crude extract of Dachengqi Decoction equivalent to the active ingredients in vivo, or used one or several active ingredients The in vitro experiments of monomers are used to speculate its mechanism of action in vivo. Since it cannot correctly reflect the active ingredients that act in vivo, it lacks experimental credibility and consistency with the actual drug action mechanism in vivo. ...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K36/708A61K9/19A61P1/00A61P43/00A61K33/04G01N30/02G01N33/48
Inventor 齐清会张栋梁李毅梁国刚
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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