Method for preparing compound biotin chitosan film and application of the same in reactivation of periodontium

A technology of chitosan film and chitosan film, which is applied to the preparation method of composite biotinylated chitosan film and the application field in periodontal tissue regeneration, can solve the problem of encapsulation research without chitosan microspheres , It is difficult to achieve complete regeneration of periodontal tissue, etc., to achieve great social and economic benefits, to achieve complete regeneration, and to restore periodontal function

Inactive Publication Date: 2008-04-02
JIANGSU PROVINCE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (3) Biotinylated barrier membrane: the above two types of GTR barrier membranes are simple barrier membranes, which can only provide a physical barrier effect, and it is difficult to achieve complete regeneration of periodontal tissue after treatment, so biotinylated barrier membranes have gradually become the current research trends
[0012] At present, there is no report on the application of chitosan microsphere-encapsulated growth factors in periodontal tissue regeneration; there is no research on chitosan microsphere-encapsulated bFGF or rhBMP2; and chitosan microsphere-containing biotin Study on Chitosan Membrane

Method used

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  • Method for preparing compound biotin chitosan film and application of the same in reactivation of periodontium

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Embodiment 1

[0035] A composite biotinylated chitosan membrane comprising human recombinant bone morphogenic protein-2 (rhBMP 2 ) and basic fibroblast growth factor (bFGF), human recombinant bone morphogenic protein-2 and basic fibroblast growth factor are all encapsulated by chitosan microspheres; human recombinant bone morphogenic protein-2 chitosan microspheres and Basic fibroblast growth factor chitosan microspheres are wrapped in complex biotinylated chitosan membrane.

[0036] The preparation method of above-mentioned a kind of compound biotinylated chitosan film, comprises the following steps:

[0037] 1) encapsulating human recombinant bone morphogenetic protein-2 with chitosan microspheres to make human recombinant bone morphogenic protein-2 chitosan microspheres;

[0038] 2) wrapping basic fibroblast growth factor with chitosan microspheres to make basic fibroblast growth factor chitosan microspheres;

[0039]3) Human recombinant bone morphogenic protein-2 chitosan microspheres...

Embodiment 2

[0045] Yet another composite biotinylated chitosan film, which includes human recombinant bone morphogenetic protein-2, which is wrapped by chitosan microspheres; human recombinant bone morphogenic protein-2 chitosan microspheres The spheres were encapsulated in a biotinylated chitosan membrane.

[0046] Another kind of preparation method of composite biotinylated chitosan film comprises the following steps:

[0047] 1) encapsulating human recombinant bone morphogenetic protein-2 with chitosan microspheres to make human recombinant bone morphogenic protein-2 chitosan microspheres;

[0048] 2) adding human recombinant bone morphogenic protein-2 chitosan microspheres into chitosan-acetic acid solution, and drying at 4°C to prepare a composite biotinylated chitosan film.

[0049] Application of yet another composite biotinylated chitosan film in periodontal tissue regeneration.

Embodiment 3

[0051] Another composite biotinylated chitosan film, which includes basic fibroblast growth factor, said basic fibroblast growth factor is wrapped by chitosan microspheres; in basic fibroblast growth factor chitosan The microspheres are coated with a biotinylated chitosan membrane.

[0052] A kind of preparation method of composite biotinylated chitosan membrane comprises the following steps:

[0053] 1) wrapping basic fibroblast growth factor with chitosan microspheres to make basic fibroblast growth factor chitosan microspheres;

[0054] 2) adding basic fibroblast growth factor chitosan microspheres into chitosan acetic acid solution, and drying at 4° C. to form a composite biotinylated chitosan film.

[0055] Application of another composite biotinylated chitosan film in periodontal tissue regeneration.

[0056] The preparation method is described in detail below:

[0057] rhBMP 2 and bFGF chitosan microspheres were prepared as follows:

[0058] Chitosan microspheres w...

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Abstract

The invention discloses a method of preparing a composite biotinylated chitosan film and application of the method in peridontal tissue regeneration. The composite biotinylated chitosan film comprises recombinant human bone forming protein -2(rhBMP2) chitosan microspheres and basicfibroblast growth factor (bFGF) chitosan microspheres; the rhBMP2 and the bFGF are wrapped by the chitosan microspheres; the rhBMP2 and bFGF chitosan microspheres are wrapped in the composite biotinylated chitosan film, or the rhBMP2 chitosan microspheres or the bFGF chitosan microspheres are wrapped in the composite biotinylated chitosan film. The invention is capable of controlling to release the rhBMP2 and the bFGF for a long time, making sure the growth factors better display their function in regeneration of peridontal tissues, so as to realize total regeneration of the peridontal tissues and recovery of the periodontal functions.

Description

technical field [0001] The invention relates to the preparation of a composite biotinylated chitosan film and its application in periodontal tissue regeneration. Background technique [0002] Periodontal disease is one of the two major diseases of the human oral cavity. It has a high prevalence in my country and the world, and has become the primary cause of tooth extraction in adults. With the aging population in our country and the world, periodontal disease will have more prominent problems, seriously affecting people's quality of life and health, and people's requirements for periodontal treatment will increase significantly. [0003] The treatment of periodontal disease has always been one of the key areas of clinical research. The treatment of periodontal disease is not only to eliminate inflammation, but more importantly, to regenerate damaged periodontal tissue and form new-attachment. How to obtain complete regeneration of periodontal tissue is a difficult problem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K9/70A61K47/36A61P1/02
Inventor 卜寿山许金菊金颂良
Owner JIANGSU PROVINCE HOSPITAL
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