Method for ferment production of 2.3-butanediol by directly enzymolysis of plants stalk

A technology of plant straw and butanediol, which is applied in the field of microbial fermentation, can solve the problems of low concentration of 2.3-butanediol, difficult industrialization of butanediol, difficult distillation and extraction, etc., and achieves high-value utilization, abundant raw materials, and improved The effect of product content

Inactive Publication Date: 2008-04-02
INST OF PROCESS ENG CHINESE ACAD OF SCI
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although it is economically feasible to use lignocellulosic waste as a carbon source from natural renewable resources, due to the high price of cellulase and the problems of fermentation inhibitors in hydrolyzate caused by physical and chemical pretreatment, cellulosic wastes can be It is difficult to industrialize the production of 2.3-butanediol by microbial fermentation from renewable resources
In view of this, the present invention discloses a method for producing 2.3-butanediol through microbia...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for ferment production of 2.3-butanediol by directly enzymolysis of plants stalk
  • Method for ferment production of 2.3-butanediol by directly enzymolysis of plants stalk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] From the preservation slant of the bacillus strains that can secrete cellulase and produce 2.3-butanediol ability obtained by screening, pick a ring and inoculate it on the 25ml seed medium (the volume of the triangular flask is 100ml), 30-37 ℃, 160 After cultivating for 12-24h under the shaking condition of -220rpm, transfer 7-12% of the inoculum amount into the 50ml liquid fermentation medium (the volume of the Erlenmeyer flask is 250ml) of shaking fermentation culture in which the stalk after the alkali treatment is the only carbon source. For: 30-37°C, 160-220rpm culture for 96-144h; sampling every 24h to measure cellulase filter paper enzyme activity and 2.3-butanediol content; the maximum filter paper enzyme activity of cellulase can reach 0.18-0.26IU / ml ; 2.3-Butanediol output is 0.14-0.24g / L;

[0049] After the fermentation and cultivation are completed, firstly carry out 100-120 mesh filtration to remove the residue in the fermentation broth, and after the filt...

Embodiment 2

[0053] Pick a ring from the slant of the strain test tube and inoculate it in a 25ml seed medium upper triangular flask (the volume of the triangular flask is 100ml), shake (160-220rpm, 30-37°C) for 12-24h, and inoculate according to the inoculum size of 10-13 %Transfer to alkali-treated straw as the only carbon source in the solid-state fermentation medium for shaking culture in the triangular flask (the volume of the triangular flask is 250ml), the amount of sample loaded on the solid-state fermentation medium: 10g of alkali-treated wheat straw+40ml of inorganic salt solution, culture conditions : 30-37°C, 180-220rpm shake flask (volume 250ml) cultured for 120-144h, sampling every 24h to measure cellulase filter paper enzyme activity and 2.3-butanediol content; cellulase filter paper enzyme activity can reach 2.1- 4.7IU / g dry material, the output of 2.3-butanediol can be 0.026-0.067g / g dry material; after the solid-state fermentation is completed, use n-butanol, n-butanol ace...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for producing 2,3-dihydroxybutane with fermentation through direct enzymatic hydrolysis of the stalk of plant, which comprises the following steps that: a bacillus with high cellulose activity and capable of producing 2,3-dihydroxybutane is separated from the polluted soil around the blanching workshop of Rong Cheng, Shang Dong Province, with the stalk or the wooden fiber as the sole carbon source, the bacillus is used to carry out solid fermentation to produce 2,3-dihydroxybutane. Aiming to exploit the substitute of the oil to solve the problem of the shortage of oil with the increasing price, the invention utilizes the renewable resource such as stalk to produce the key platform chemical of 2,3-dihydroxybutane through microorganism fermentation to accomplish the high-value utilization of the substances of the stalk, and overcome the puzzle of the method to produce the 2,3-dihydroxybutane at present, such as the impossibility of direct usage of cellulose as the carbon source, the fermentation inhibitor which is easily produced during the chemical-physical pretreatment, and the hard extraction due to the liquid fermentation.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for producing 2.3-butanediol by direct fermentation of straw without adding cellulase by using a bacillus strain capable of secreting cellulase. Background technique [0002] 2.3-Butanediol, as a potentially very valuable platform compound, has attracted widespread attention internationally. It is a colorless and odorless chiral compound with a molecular weight of 90.12kDa, a high boiling point (180-184°C), and a low freezing point (-60°C). It is an excellent antifreeze agent. As a chemical intermediate, it can be dehydrated into diacetyl, which is widely used in fuel additives with high combustion value, and 1.3-butadiene, which is widely used in synthetic rubber; other compounds can be generated through condensation, polymerization and other reactions , such as: styrene, octane and 2.3-butanediol diacetate, etc., as additives can be widely used in ink...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P7/18C12R1/07
Inventor 陈洪章孙付保
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap