Method for preparing complex nanometer fibrous tissue renovation bracket containing collagen

[0020] Example 1

[0021] Use an electronic analytical balance to weigh 0.3g of collagen and dissolve it in 5ml of hexafluoroisopropanol, stir with micro-thermal magnetic force until it is completely dissolved, and obtain a collagen spinning solution with a concentration of 6% (g/ml); use an electronic analytical balance to weigh Dissolve 0.3g of thermoplastic polyurethane in 5ml of hexafluoroisopropanol, stir with micro-thermal magnetic force until it is completely dissolved, and obtain a thermoplastic polyurethane spinning solution with a concentration of 6% (g/ml); after the two are completely dissolved, press the two The ratio of 2:8 is evenly mixed, and the total concentration of the mixed solution is 6%; the electrospinning process is: spinning voltage is 12kv, syringe pump advancement speed is 1.5ml/h, receiving distance is 12cm, using 9 gauge needle, aluminum foil accepting Or degreased gauze accepts fibers to obtain a disordered nanofiber film with an average fiber diameter of about 345 nanometers.

[0022] Example 2

[0023] Use an electronic analytical balance to weigh 0.3g of collagen and dissolve it in 5ml of hexafluoroisopropanol, stir with micro-thermal magnetic force until it is completely dissolved, and obtain a collagen spinning solution with a concentration of 6% (g/ml); use an electronic analytical balance to weigh Dissolve 0.3g of thermoplastic polyurethane in 5ml of hexafluoroisopropanol, stir with micro-thermal magnetic force until it is completely dissolved, and obtain a thermoplastic polyurethane spinning solution with a concentration of 6% (g/ml); after the two are completely dissolved, press the two The ratio of 6:4 is mixed evenly, and the total concentration of the mixed solution is 6%; the electrospinning process is: spinning voltage is 16kv, syringe pump advancement speed is 1.2ml/h, receiving distance is 12cm, using 9 gauge needle, aluminum foil accepting Or degreased gauze accepts fibers to obtain a disordered nanofiber film with an average fiber diameter of about 208 nm.

[0024] Example 3

[0025] Use an electronic analytical balance to weigh 0.4g of collagen and dissolve it in 5ml of hexafluoroisopropanol, stir with micro-thermal magnetic force until it is completely dissolved, and obtain a collagen spinning solution with a concentration of 8% (g/ml); use an electronic analytical balance to weigh Dissolve 0.5g of thermoplastic polyurethane in 10ml of hexafluoroisopropanol, stir it with micro-thermal magnetic force until it is completely dissolved, and obtain a thermoplastic polyurethane spinning solution with a concentration of 5% (g/ml); after the two are completely dissolved, the volume of both The ratio of 5:8 is mixed evenly, and the total concentration of the mixed solution is 6.2%; the electrospinning process is: spinning voltage is 16kv, syringe pump advancement speed is 1.2ml/h, receiving distance is 12cm, and No. 9 needle is selected. The aluminum foil accepts or the degreased gauze accepts the fibers to obtain a disordered nanofiber film with a thickness of 0.08 nanometers.