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Method for biological molecule detection by nanometer gold magnetic particle

A technology of biomolecules and gold magnetic particles, which is applied in the fields of biochemical equipment and methods, measurement/inspection of microorganisms, biological testing, etc., can solve the problem that it is difficult to obtain quantum dots, the fluorescence life of quantum dots is short, and nanoscale magnetic composites are not involved. Problems such as the optical properties of particles, to achieve the effect of convenient detection results

Active Publication Date: 2012-08-29
XIAN GOLDMAG NANOBIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there have been many reports on the detection of biomolecules using the characteristic optical absorption of gold nanoparticles, the labeling technology of fluorescent substances, and the fluorescence properties of quantum dots, but there are inconvenient separation and fluorescence of biomolecules labeled with gold nanoparticles Due to the harshness of substances to biomolecular labeling conditions, and the fact that the fluorescence wavelength of quantum dots is related to its particle size, it is difficult to obtain quantum dots with uniform particle size and crystal defects with the current technology, and quantum dots have defects such as short fluorescence lifetime and high price.
[0003] The preparation method and structural composition of assembled magnetic composite particles and core / shell superparamagnetic composite particles have been disclosed in Chinese patents ZL03153486.4 and ZL03124061.5 respectively, but they do not involve the optical properties of nanoscale magnetic composite particles, nor Methods not involved in the detection of biomolecules with this optical property

Method used

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  • Method for biological molecule detection by nanometer gold magnetic particle
  • Method for biological molecule detection by nanometer gold magnetic particle
  • Method for biological molecule detection by nanometer gold magnetic particle

Examples

Experimental program
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Embodiment 1

[0055] The purpose of this example is to illustrate a rapid visual and intuitive detection of the optical properties of nano-gold magnetic particles by immobilizing antibodies on the surface of gold magnetic particles and capturing target molecules under the control of an external magnetic field in the present invention.

[0056] 1. Pretreatment of gold magnetic particles: take about 50nm, 500μL (500μg) gold magnetic particles for magnetic separation on a magnetic separator, discard the supernatant, wash it twice with coupling buffer and store in coupling buffer solution, and measure the position of the absorption peak of the gold magnetic particles on a UV-Vis spectrophotometer;

[0057] 2. Immobilize the antibody on the surface of the gold magnetic particles: add 300 μg of human IgG to the gold magnetic particles in the 0.05M Tris-HCl coupling buffer with a pH of 7.4, and place it in an air constant temperature oscillator at 37°C and 150r / min Fully react in 30min;

[0058] ...

Embodiment 2

[0063] This example aims to illustrate a fast visual and intuitive detection of the optical properties of nano-gold magnetic particles by immobilizing nucleic acids on the surface of gold magnetic particles in the present invention, and then hybridizing with target nucleic acid fragments under the control of an external magnetic field.

[0064] 1. Pretreatment of gold magnetic particles: take about 50nm, 500μL (500μg) gold magnetic particles for magnetic separation on a magnetic separator, discard the supernatant, wash it twice with coupling buffer and store in coupling buffer solution, and measure the position of the absorption peak of the gold magnetic particles on a UV-Vis spectrophotometer;

[0065] 2. Fix nucleic acid on the surface of gold magnetic particles:

[0066] 2.1. Add 200 μg of mouse anti-biotin antibody to gold magnetic particles in 0.05M Tris-HCl buffer system with a pH of 7.4, and place it in an air constant temperature oscillator at 37°C and 180r / min to full...

Embodiment 3

[0073] The purpose of this example is to illustrate the rapid visual and intuitive detection of the optical properties of nano-gold magnetic particles by immobilizing polysaccharides on the surface of gold magnetic particles and capturing target molecules under the control of an external magnetic field in the present invention.

[0074] 1. Pretreatment of gold magnetic particles: take about 50nm, 5mL (5mg) gold magnetic particles for magnetic separation on a magnetic separator, discard the supernatant, and use 0.05M, pH 6.0 sodium acetate acetic acid coupling buffer to separate It is washed twice and stored in a coupling buffer, and the position of the absorption peak of the gold magnetic particles is measured on an ultraviolet-visible spectrophotometer;

[0075] 2. Fix the polysaccharide on the surface of the gold magnetic particles: Add 100mM, 1mL mannose modified with amino groups to the gold magnetic particles in 0.05M, pH 6.0 sodium acetate-acetic acid coupling buffer, pla...

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Abstract

The method comprises: 1) fixing the biomolecules; 2) closing off the fixed biomolecules; 3) testing the target biomolecules. The invention uses characteristic of absorption spectrum owned by GoldMag nano-particles, and the separability of external magnetic field and better monodispersion and stability to build the method of using GoldMag nano-particle to test biomolecules.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for detecting biomolecules by using nano-gold magnetic particles. Background technique [0002] With the rapid development of science and technology, nanotechnology has become more and more widely used. The optical properties of nanomaterials used in the detection of biomolecules have gradually entered the stage of science and technology. At present, there have been many reports on the detection of biomolecules using the characteristic optical absorption of gold nanoparticles, the labeling technology of fluorescent substances, and the fluorescence properties of quantum dots, but there are inconvenient separation and fluorescence of biomolecules labeled with gold nanoparticles Due to the harshness of substances to biomolecular labeling conditions, and the fact that the fluorescence wavelength of quantum dots is related to its particle size, it is difficult to obtain qu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N21/64G01N21/78C12Q1/68
Inventor 崔亚丽陈超惠文利辛小芳
Owner XIAN GOLDMAG NANOBIOTECH
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