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Continuous coupled solid-state fermentation method and biological reactor

A biological reaction device, solid-state fermentation technology, applied in the field of fermentation industry to save manpower and material resources

Inactive Publication Date: 2008-05-14
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Suitable for continuous solid state fermentation process

Method used

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  • Continuous coupled solid-state fermentation method and biological reactor

Examples

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Effect test

example 1

[0022] Example 1: Continuous fermentation of enzymatic hydrolysis, fermentation and air stripping coupled with steam-exploded straw fermentation to produce cellulose ethanol

[0023] Material preparation: cut the corn stalks to a length of 2-3 cm, maintain 1.4 MPa in the steam explosion tank for 5 minutes, release the pressure instantly, and obtain the steam explosion straw.

[0024] Sterilization: Steam-exploded straw is sterilized in another container at 121-125°C for 15-20 minutes. Vertical tank and horizontal tank are sterilized, enter steam from (5)(8), exhaust steam from (6)(9), maintain the pressure at 121-125°C for 15-20min.

[0025] Mixing, feeding and enzymatic hydrolysis: by passing CO before feeding 2 Air in the replacement tank, (5)(8) intake, (6)(9) exhaust. The sterilized and cooled steam-exploded straw is mixed with cellulase at a ratio of 20μ / g-steam-exploded straw, and the water content is adjusted to 65-75%. According to the ratio of adding 1g of yeast fo...

Embodiment 2

[0027] Example 2: Enzymolysis, fermentation and gas-solid separation coupled continuous fermentation of steam-exploded wheat straw solid-state fermentation to produce hydrogen

[0028] Material preparation: cut the wheat straw to a length of 15-20 cm, maintain 1.4 MPa in the steam explosion tank for 5 minutes, release the pressure instantly, and obtain the steam explosion wheat straw.

[0029] Sterilization: Put the steam-exploded wheat straw into the sterilizer for sterilization, the sterilization temperature is 121-125°C, and the sterilization time is 15-20min; the solid-state fermentation reactor is sterilized by steam, and the steam is from (5) (8 ) enters, flows out from (6)(9), and maintains at 121-125°C for more than 30 minutes.

[0030] Enzymatic hydrolysis: prepare cellulase into a 100μ / ml solution, and then mix the solution with sterilized and cooled steam-exploded wheat straw at a ratio of 20μ / g. Adjust the temperature of the horizontal tank to maintain 35±1°C. Op...

Embodiment 3

[0034] Example 3: Fermentation, gas stripping coupled continuous fermentation for the production of ethanol by solid-state fermentation of sweet sorghum

[0035] Material preparation: remove the sorghum ears, sorghum leaves and roots from the mature harvested sweet sorghum stalks and crush them;

[0036] Sterilization: Put the crushed sweet sorghum stalks into a sterilizing pot for sterilization, the sterilization temperature is 121-125°C, and the sterilization time is 15-20min; the solid-state fermentation reactor is sterilized by steam, and the steam is from (5) Enter, flow out from (6), maintain 121-125°C for more than 30 minutes.

[0037] Fermentation: Mix Angel yeast (purchased in the market) with sterile water, the mixing ratio is 1g of yeast plus 40mL of sterile water, activate at 35°C for 25-30min, and then place it at room temperature for 45-60min after activation; In the sterilized container, mix the sweet sorghum with the activation solution according to the ratio ...

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Abstract

The invention discloses a continuous-coupling solid fermentation method and a solid bioreactor thereof. The invention has the operation method that: material is fermented in a horizontal tank first and then extracted in a vertical tank; or the material first finishes an enzymatic hydrolysis process in the horizontal tank, and then is fermented and extracted in the vertical tank. The bioreactor is characterized in that: a material inlet is provided with an airlock that can cause the material to continuously enter a fermentation tank and separate the fermentation tank with outside; the enzymatic hydrolysis, the fermentation and the extraction processes of the material are integrated into one reactor and are continuously done. The invention is suitable for the solid fermentation processes such as biomass alcohol, biomass hydrogen-production, fermentation alcohol by sweet sorghum, fermentation xanthan by inert carrier, etc.; the method realizes the continuously fermentation and coupling of every process stage; compared with fermentation in batch, the steps of reactor inoculation, cleaning and sterilization, etc., are reduced and the middle operation are also spared, thus saving manpower and material; in addition, the whole process has no turning and stirring device for solid material, thus avoiding the damage to mycelium.

Description

technical field [0001] The invention belongs to the technical field of fermentation industry Background technique [0002] Solid state fermentation is a fermentation process with little or no free water. Humans have long applied solid-state fermentation technology to food production, and almost all ancient fermented products were produced using solid-state fermentation technology. Compared with liquid fermentation, solid-state fermentation has the advantages of low energy consumption, less pollution, and environmental friendliness. Therefore, solid-state fermentation has been more and more used in research and production in recent years. [0003] The research and application of solid-state fermentation depends on the continuous development and improvement of fermentation technology and bioreactors used in fermentation. Traditional solid-state fermentation is not purebred cultivation, and its fermentation process is carried out in an open fermentation tank. With the advanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00
Inventor 陈洪章张志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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