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Effective component of angelica as well as preparation method and application

An effective component, the technology of angelica, is applied in the application field of preparation, treatment and prevention of tumor drugs, which can solve the problems of side effects and involvement of normal cells, etc., and achieve the effect of increasing the content, simple and clear chemical composition, and good pharmacological activity

Inactive Publication Date: 2008-05-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of angelica as well as preparation method and application
  • Effective component of angelica as well as preparation method and application
  • Effective component of angelica as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of the effective component of embodiment one Angelica sinensis

[0027] Add ethyl acetate and ethanol after crushing 200g of angelica medicinal material, heat and extract to obtain the extract, concentrate the extract into 10.2g of extract, and mix it with silica gel as a sample, and separate it with a normal phase silica gel column. Petroleum ether and ethyl acetate are used as mobile phase to obtain eluent I, discard it, then change chloroform and methanol as mobile phase to obtain eluent II, and obtain 3.3g of sample after eluent II is concentrated and dried; Phase chromatography continues to separate the obtained samples; separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, and gradient elution is performed. The sample was dissolved in 100% ethanol, separated by preparative liquid chromatography, and the solution was collected in a period of 34.2 to 44.0 m...

Embodiment 2

[0028] The preparation of embodiment two angelica effective components

[0029] Add ethyl acetate and ethanol (1:0.8 to 1.2) after crushing 500g of Angelica sinensis, heat and reflux for 0.8 to 1.2 hours, extract 1 to 3 times, combine the filtrates to obtain an extract, concentrate the extract to 21.2g of extract, and Mix it with silica gel, and separate it with a normal phase silica gel column. First, use petroleum ether and ethyl acetate (47-53:1) as the mobile phase to obtain eluent I, discard it, and then replace it with chloroform and Methanol (8~13:1) was used as mobile phase to obtain eluent II, and after concentrating and drying eluent II, 6.8g of sample was obtained; the sample obtained by continuing to separate with preparative liquid chromatography; the separation condition of preparative chromatography: chromatographic column To prepare the column, the mobile phase is water and acetonitrile, gradient elution, the flow rate is 9-11ml / min, and the column temperature ...

Embodiment 3

[0030] The preparation of embodiment three angelica effective components

[0031] Take 250 g of Angelica sinensis, crush it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extract to obtain 10.5g, the extract and silica gel are mixed as a sample, and the normal phase silica gel column is used for separation, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 3.5 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is an Agient preparative column (Zorbax SB-C 18; 21.2mm × 250mm), mobile phase is water (A) and acetonitrile (B), and the gradient elution program ...

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Abstract

The invention provides an effective component of angelica, mainly including a composite A: Japoangelol D, a composite B: Angelicolide and a composite C: Angeolide, wherein, the content is respectively 15 to 30 percent, 60 to 75 percent and 10 to 25 percent. The effective component is made through the steps of: heating and extracting, concentrating, silica column separation, eluting, concentrating and drying of the eluting liquid, continuously separating by liquid phase chromatogram, collecting the solution and concentrating and drying the solution. The effective component of the angelica disclosed by the invention is applicable for the preparation of medicines for curing and preventing tumor. The invention discloses the method which is reasonably designed to acquire the effective components rapidly and accurately, moreover, the quality of the medicine can be easily controlled in production.

Description

technical field [0001] The invention belongs to traditional Chinese medicine extract, and in particular relates to an effective component extracted from Angelica sinensis, a preparation method thereof, and an application of the effective component in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tum...

Claims

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Application Information

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IPC IPC(8): A61K36/232A61K31/365A61K9/20A61K9/48A61P35/00
Inventor 贺庆程翼宇瞿海斌刘雳水文波葛志伟窦静
Owner ZHEJIANG UNIV
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