Method for separating and extracting sweet tea tannins

A sweet tea polyphenol and extraction method technology, applied in the field of separation and extraction of sweet tea polyphenols, can solve the problem that the separation and extraction method has not been reported, and achieve the effects of obvious anti-allergic activity and simple operation.

Inactive Publication Date: 2008-06-04
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although sweet tea polyphenols have been applied in Japan and other countries, the industrialized separation and extraction method has not been reported, and the separation and purification research, industrial production and application of sweet tea polyphenols are still blank in China.

Method used

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  • Method for separating and extracting sweet tea tannins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Add 100 grams of sweet tea dry leaves, add 3000ml of distilled water, extract at 95°C for 20 minutes, filter to remove slag to obtain tea juice. After the tea juice is cooled, adjust the pH to 8.5 with 1% NaOH solution, then heat the tea juice to boil and cool it, centrifuge to remove insoluble matter to obtain 2750ml clear tea juice, adjust the pH of the tea juice to 5.4 with 1% HCL solution. Organic membranes with molecular weight cut-offs of 50,000 and 5,000 were used for ultrafiltration twice, and the retentate between 5,000 and 50,000 was concentrated and freeze-dried to obtain 16.1 grams of sweet tea powder with a content of sweet tea polyphenols of 50.2%.

Embodiment 2

[0033] 100 grams of sweet tea dry leaves, add 1000ml of distilled water to extract as in Example 1, transfer to dissolve, ultra-filter to obtain the retentate and concentrate, then use LSA-21 chromatography column to adsorb, use ethanol aqueous solution to carry out gradient elution, collect 70% The eluate was concentrated and freeze-dried to obtain 1.9 grams of sweet tea powder, with a sweet tea polyphenol content of 90.2%.

Embodiment 3

[0035] Add 100 grams of sweet tea dry leaves, add 3000 ml of 60% ethanol aqueous solution, extract at 60°C for 60 minutes, filter to remove residue, evaporate and recover ethanol to obtain 600 ml of tea juice. Add 1200ml of distilled water into the tea juice, stir and filter, take the clear juice and perform two ultrafiltrations, the molecular weight cut-offs of the filter membranes are 10000 and 1000 respectively. The retentate between 1000 and 10000 was concentrated, freeze-dried to obtain 16.7 grams of sweet tea powder, and the content of sweet tea polyphenols was 52.1%.

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Abstract

The invention relates to the separation and extraction methods of a sweet tea polyphenols. The invention uses integration of ultrafiltration with resin adsorption separation technique. Only ethanol is used as organic solvent during producing process and the separation technique of sweet tea polyphenols is optimized; the simple operation helps realize industrialized production; besides, the production process accords with environment protection requirement and the products accord with food and drug raw material requirements. The invention uses Guangxi sweet tea as raw materials to produce sweet tea polyphenols containing over 50% and 90%. Hyaluronidase inhibition in vitro experiment, in vivo experiments of mice passive dermato-allergic reaction and the delayed cutaneous hypersensitivity caused by dinitro-chlorobenzene confirm the invention has significant anti-anaphylaxis activity.

Description

technical field [0001] The invention relates to a method for separating and extracting sweet tea polyphenols from sweet tea. Background technique [0002] Sweet tea (Rubus suavissims S. Lee) is a perennial deciduous shrub of the genus Rubus in the family Rosaceae. Wild sweet tea resources are distributed in Jinxiu, Luzhai, Zhaoping, Cenxi, Cangwu, Tengxian, Hexian, Guiping and other counties and cities in Guangxi. They are born in evergreen broad-leaved forests or valleys, forest edges, In the bushes, it is a unique plant in Guangxi. [0003] Sweet tea has a long history of folk use in Guangxi. For a long time, sweet tea has been used not only as tea for drinking, but also for medicinal purposes. Sweet tea is cool in nature, non-toxic, has the effects of promoting body fluid and relieving cough, clearing away heat and detoxification, relieving throat and moistening lung, reducing inflammation and blood pressure, lowering blood sugar, losing weight, and anti-allergic. Liao...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P3/10C07D311/22C07H17/00
Inventor 滕建文韦保耀黄丽夏宁
Owner GUANGXI UNIV
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