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Method for preparing schisandra active component polysaccharides

The technology of an active ingredient, Schisandra chinensis, is applied in the field of Schisandra chinensis polysaccharide, which is a functional active ingredient of Schisandra chinensis. It can solve the problems that are not conducive to the separation of active ingredients, the physiological activity of polysaccharides, and the easy destruction of polysaccharide structures, so as to reduce the number of removal times, improve the extraction rate, and improve the process. reasonable effect

Active Publication Date: 2008-06-18
DALIAN POLYTECHNIC UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above research and patent reports on Schisandra polysaccharides are all reports on the whole fruit of Schisandra chinensis, and most of them are the dried fruits of Schisandra chinensis studied, and there are no reports of fresh fruits
In view of the functional components lignin and polysaccharides of Schisandra chinensis, the contents of different parts have important differences. The polysaccharides of Schisandra chinensis are mostly concentrated in the pulp, and 90% of the lignin is concentrated in the seeds. Using the whole fruit to extract polysaccharides is not conducive to the activity Separation of components and easy waste of resources
In addition, the above reports all use the hot water extraction method to extract polysaccharides, and there are few reports on the method of removing protein. Some use trichloroacetic acid to remove protein. Because of its strong acidity, it is easy to destroy the polysaccharide structure, which is not conducive to the protection Physiological activity of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] Wash and spray 500 g of fresh fruit of Schisandra chinensis, remove impurities and moldy fruit, heat to 60° C., beat with a multi-channel beater to obtain 380 g of fruit pulp. Add 0.4g (1.5% of the total solids) of cellulase (50,000 units / g), at 30°C, enzymatically hydrolyze for 5h; heat up to 90°C to inactivate the enzyme for 10min, cool to room temperature, centrifuge at 4000r / min for 10min, Obtain supernatant and precipitate; add 1200g of water to 80g of precipitate, extract at 70°C for 5 hours, cool to room temperature, centrifuge at 4000r / min for 10min, obtain supernatant and precipitate; add 50g of precipitate, add 250g of water, extract at 100°C for 2 hours , centrifuged at 4000r / min for 10min, the precipitate was extracted with 250g of water once, and the combined extract was 1500g. Concentrate to a volume of 300ml (1 / 5 of the original volume), slowly add the concentrated solution to 900ml of 95% ethanol solution, settling with alcohol at 4°C for 12h; centrifugi...

example 2

[0034]Pick 100g of dried fruit of Schisandra chinensis, wash, add 500g of water to soak for 12 hours, heat to 80°C, and beat with a multi-channel beater to obtain 480g of fruit pulp. Add 1.008g (3.0% of the total solids) of cellulase (50,000 units / g), at 50°C, enzymatically hydrolyze for 1h; heat up to 90°C to inactivate the enzyme for 10min, cool to room temperature, centrifuge at 4000r / min for 10min, Obtain supernatant and precipitate; precipitate 90g, add water 1000g, temperature 90°C, extract for 3h, cool to room temperature, centrifuge at 4000r / min for 10min, obtain supernatant and precipitate; precipitate 60g, add water 600g, temperature 100°C, extract for 2h, 4000r / min centrifugation for 10min, and extract a total of 1800g. Concentrate to a volume of 400ml (2 / 9 of the original volume), slowly add the concentrated solution to 1600ml of 95% ethanol solution, and alcohol precipitation at 4°C for 12h; centrifuge to obtain 38g of precipitate. Dissolve the precipitate in 60...

example 3

[0036] Wash and spray 500 g of fresh fruit of Schisandra chinensis, remove impurities and moldy fruit, heat to 60° C., and beat with a multi-channel beater to obtain 360 g of fruit pulp. Add 0.58g (2.0% of the total solids) of cellulase (50,000 units / g), at 40°C, enzymatically hydrolyze for 3h; heat up to 90°C to inactivate the enzyme for 10min, cool to room temperature, centrifuge at 4000r / min for 10min, Obtain supernatant and precipitate; precipitate 70g, add water 1000g, temperature 100°C, extract for 3h, cool to room temperature, centrifuge at 4000r / min for 10min, obtain supernatant and precipitate; precipitate 48g, add water 720g, temperature 100°C, extract for 3h, 4000r / min centrifuged for 10min, and the combined extract was 1750g. Concentrate to a volume of about 440ml (1 / 4), slowly add the concentrated solution to 2200ml of 95% ethanol solution, settling with alcohol for 12 hours at 4°C; centrifuge to obtain 34 grams of precipitate, dissolve the precipitate in 500g of...

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PUM

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Abstract

A preparation method of active component in schisandra chinensis, including the preparation steps of the raw material treatment, the polysaccharide extraction, the ethanol classification, the protein removal, the alcohol precipitation and the drying. The raw material treatment is to immerse fresh schisandra or dry schisandra and separate fruit pulp and seeds after the multiple beating; the polysaccharide extraction is to use one or two of cellulose and pectinase for the enzymatic hydrolysis which are extracted with water; the ethanol classification is to add polysaccharide solution in ethanol solution; the protein removal method is to combine acid protease with Sevag. The invention provides a method of extracting the active component polysaccharide from the schisandra, especially paves a way for extracting the active component by a fresh fruits extraction method and a seed-fruit separation method. Both resources and energies are saved and the extraction cost is lowered. And the invention provides the technical support to the reasonable development of the schisandra.

Description

technical field [0001] The invention relates to a production method of schisandra polysaccharide, a functional active ingredient of schisandra chinensis. Background technique [0002] Schisandra chinensis (Tarcz.) Bail1. is the mature fruit of Magnoliaceae Schisandra chinensis (Tarcz.) Bail1. Since the 1950s, scholars from China, Japan, the former Soviet Union and other countries have used modern scientific methods to do a lot of research, proving that Schisandra can reduce serum alanine aminotransferase in patients with hepatitis; treat chemical poison damage to the liver; have an excitatory effect on the central nervous system , can enhance the defense ability of the body to non-specific stimuli; increase the blood pressure of patients with hypotension, but not normal blood pressure; affect the excitation and inhibition of the cerebral cortex, improve people's intelligence, increase memory; enhance the function of the adrenal cortex, Promotes heart activity. Its function...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04A61K36/57A61K131/00
Inventor 朱蓓薇毛丰美李冬梅王丽辉于运海董秀萍阎雪
Owner DALIAN POLYTECHNIC UNIVERSITY
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