Method for preparing schisandra active component polysaccharides
The technology of an active ingredient, Schisandra chinensis, is applied in the field of Schisandra chinensis polysaccharide, which is a functional active ingredient of Schisandra chinensis. It can solve the problems that are not conducive to the separation of active ingredients, the physiological activity of polysaccharides, and the easy destruction of polysaccharide structures, so as to reduce the number of removal times, improve the extraction rate, and improve the process. reasonable effect
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example 1
[0032] Wash and spray 500 g of fresh fruit of Schisandra chinensis, remove impurities and moldy fruit, heat to 60° C., beat with a multi-channel beater to obtain 380 g of fruit pulp. Add 0.4g (1.5% of the total solids) of cellulase (50,000 units / g), at 30°C, enzymatically hydrolyze for 5h; heat up to 90°C to inactivate the enzyme for 10min, cool to room temperature, centrifuge at 4000r / min for 10min, Obtain supernatant and precipitate; add 1200g of water to 80g of precipitate, extract at 70°C for 5 hours, cool to room temperature, centrifuge at 4000r / min for 10min, obtain supernatant and precipitate; add 50g of precipitate, add 250g of water, extract at 100°C for 2 hours , centrifuged at 4000r / min for 10min, the precipitate was extracted with 250g of water once, and the combined extract was 1500g. Concentrate to a volume of 300ml (1 / 5 of the original volume), slowly add the concentrated solution to 900ml of 95% ethanol solution, settling with alcohol at 4°C for 12h; centrifugi...
example 2
[0034]Pick 100g of dried fruit of Schisandra chinensis, wash, add 500g of water to soak for 12 hours, heat to 80°C, and beat with a multi-channel beater to obtain 480g of fruit pulp. Add 1.008g (3.0% of the total solids) of cellulase (50,000 units / g), at 50°C, enzymatically hydrolyze for 1h; heat up to 90°C to inactivate the enzyme for 10min, cool to room temperature, centrifuge at 4000r / min for 10min, Obtain supernatant and precipitate; precipitate 90g, add water 1000g, temperature 90°C, extract for 3h, cool to room temperature, centrifuge at 4000r / min for 10min, obtain supernatant and precipitate; precipitate 60g, add water 600g, temperature 100°C, extract for 2h, 4000r / min centrifugation for 10min, and extract a total of 1800g. Concentrate to a volume of 400ml (2 / 9 of the original volume), slowly add the concentrated solution to 1600ml of 95% ethanol solution, and alcohol precipitation at 4°C for 12h; centrifuge to obtain 38g of precipitate. Dissolve the precipitate in 60...
example 3
[0036] Wash and spray 500 g of fresh fruit of Schisandra chinensis, remove impurities and moldy fruit, heat to 60° C., and beat with a multi-channel beater to obtain 360 g of fruit pulp. Add 0.58g (2.0% of the total solids) of cellulase (50,000 units / g), at 40°C, enzymatically hydrolyze for 3h; heat up to 90°C to inactivate the enzyme for 10min, cool to room temperature, centrifuge at 4000r / min for 10min, Obtain supernatant and precipitate; precipitate 70g, add water 1000g, temperature 100°C, extract for 3h, cool to room temperature, centrifuge at 4000r / min for 10min, obtain supernatant and precipitate; precipitate 48g, add water 720g, temperature 100°C, extract for 3h, 4000r / min centrifuged for 10min, and the combined extract was 1750g. Concentrate to a volume of about 440ml (1 / 4), slowly add the concentrated solution to 2200ml of 95% ethanol solution, settling with alcohol for 12 hours at 4°C; centrifuge to obtain 34 grams of precipitate, dissolve the precipitate in 500g of...
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