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Natural sex hormone extracted by supercritical CO2 extraction method from pilose antler

A supercritical and deer antler technology, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, steroids, etc., can solve the problems of toxic dosage, long extraction cycle, and high temperature requirements, and achieve high extraction rate and recovery rate. The effect of short operation time and simple process conditions

Inactive Publication Date: 2008-07-02
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all have the disadvantages of long extraction period, the solvent used has certain toxicity and a large amount, requires high temperature and degrades or denatures most active substances of velvet antler, and the extraction rate and recovery rate of the final target product are very low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Freeze-dry to constant weight and crush 100g of velvet antler powder with a particle size of 40-80 mesh, add 100ml of an ethanol solution with a mass concentration of 85%, and put it into the extraction kettle. The extraction temperature is 35°C, the extraction pressure is 40MPa, CO 2 Static extraction was carried out for 90 minutes at a flow rate of 2.0 L / min, after which CO 2 Enter the extraction tank at a flow rate of 2.0L / min, and perform dynamic extraction for 60min at an extraction temperature of 35°C and an extraction pressure of 40MPa. The extract is separated in the extraction tank to remove carbon dioxide under the conditions of an operating temperature of 35° C. and an operating pressure of 10 MPa. The extract solution obtained after separation was evaporated and dried under reduced pressure at 50° C. at 0.01 MPa to obtain a yellow paste containing estradiol and progesterone. The total extraction rate reached 3.79%. The extraction rates of ketones in deer an...

Embodiment 2

[0014] Add 100g of velvet antler powder freeze-dried to constant weight and crushed with a particle size of 100-120 mesh, add 100ml of ethanol with a mass concentration of 100%, and put it into the extraction kettle. The extraction temperature is 80°C, the extraction pressure is 30MPa, CO 2 Static extraction was performed for 90 minutes at a flow rate of 2.0 L / min. Afterwards, add 100% ethanol continuously and dynamically at a flow rate of 1.5ml / min, and open the outlet valve to maintain an ethanol flow rate of 1.5ml / min. The extraction temperature is 80°C, the extraction pressure is 30MPa, and the carbon dioxide flow rate is 2.0L / min. Carry out dynamic extraction for 60min. The extract is separated in the extraction kettle to remove carbon dioxide under the condition of operating temperature of 35° C. and operating pressure of 6 MPa. The extract solution that obtains after separation is at 50 ℃, 0.01MPa evaporates and dries under reduced pressure to obtain the tan paste that...

Embodiment 3

[0016] Freeze-dried to constant weight and crushed 100g of velvet antler powder with a particle size of 200-300 mesh, add 100ml of ethanol with a mass concentration of 50%, and then put it into the extraction kettle. The extraction temperature is 60°C, the extraction pressure is 30MPa, CO 2 Static extraction was performed for 90 minutes at a flow rate of 2.0 L / min. Afterwards, add 50% ethanol continuously and dynamically at a flow rate of 0.5ml / min, and open the outlet valve to maintain an ethanol solution flow rate of 0.5ml / min. The extraction temperature is 60°C, the extraction pressure is 30MPa, and the carbon dioxide flow rate is 2.0L / min. Under the conditions of dynamic extraction for 60min, the extract was separated and removed carbon dioxide in the extraction tank under the condition of operating temperature of 50°C and operating pressure of 8MPa. The extract solution that obtains after separation is at 60 ℃, 0.01MPa evaporates and dries under reduced pressure to obtain...

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Abstract

The invention discloses a supercritical CO2 extraction method of natural hormones from pilose antler. The method includes that: the crushed freeze-dried pilose antler is added in ethanol solution and then is added in an extraction kettle to carry out the static extraction at the extraction temperature of 35 to 80 DEG C, the extraction pressure of 10 to 40MPa and the carbon dioxide flow rate of 0.5 to 2.0L / min, then the same carbon dioxide flow rate is maintained to carry out the dynamic extraction, or ethanol solution is continuously added in the extraction kettle to carry out the dynamic extraction after the static extraction, and the extract is carried out the separation, decompression evaporation and vacuum drying so as to obtain the yellowish brown paste substance containing estradiol, progesterone, phospholipid, alkaloid components and p-hydroxybenzaldehyde. The method has simple process, fast extraction speed and high recovery rate, and the obtained extract can be used for the health care products or drugs for the prevention and treatment of female menopause syndrome.

Description

technical field [0001] The present invention relates to a kind of supercritical CO 2 The method for extracting natural sex hormones in deer antler belongs to the technology of extracting natural sex hormones. Background technique [0002] Deer antler is a precious animal medicinal material, which is the young horns of male sika deer or red deer with dense hair and not yet ossified. Antler contains phospholipids, natural sex hormones, fatty acids, amino acids, polysaccharides, polypeptides, proteins and inorganic salts, which have the functions of warming kidney and strengthening yang, nourishing essence and blood, strengthening muscles and bones. [0003] Modern research has proved that deer antler contains various natural sex hormones such as estradiol and progesterone. In the existing literature, about the extraction process of velvet antler neutral sex hormones, methanol, ether or acetone are generally used as extraction solvents for heat reflux and vibration extraction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/32C07J1/00C07J5/00
CPCY02P20/54
Inventor 李淑芬周冉徐志红张大成田松江
Owner TIANJIN UNIV
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