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Microwave method for extracting beta-carotene from tobacco leaf

A carotene and extraction method technology, applied in the direction of organic chemical methods, chemical instruments and methods, tobacco, etc., can solve the problems of high energy consumption for raw material pretreatment, easy destruction of heat-sensitive components, and high labor intensity, etc., and achieve low cost, The effect of developing utilization value and improving utilization rate

Active Publication Date: 2008-07-02
江西润井康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the main methods used to extract β-carotene at home and abroad are: organic solvent extraction, enzyme reaction, microbial fermentation, artificial synthesis, supercritical CO 2 Extraction method, etc., the most commonly used method is solvent extraction method (i.e. leaching method), but the traditional leaching method has disadvantages such as long leaching time, high labor intensity, high energy consumption for raw material pretreatment, and easy damage to heat-sensitive components.

Method used

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  • Microwave method for extracting beta-carotene from tobacco leaf

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Experimental program
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Effect test

Embodiment 1

[0019] The specific process steps are as follows:

[0020] (1) Tobacco leaves are dried and crushed at 45° C. to pass through a 20-mesh sieve to obtain tobacco leaf powder.

[0021] (2) Add ethyl acetate to the tobacco leaf powder obtained in step 1, extract with microwave radiation for 90 seconds under the condition of microwave power of 100 watts, cool and filter, and obtain a crude extract.

[0022] (3) After concentrating the crude extract obtained in step 2 under reduced pressure, undergo alumina chromatography with petroleum ether as the eluent to obtain β-carotene.

Embodiment 2

[0024] The specific process steps are as follows:

[0025] (1) Tobacco leaves are dried and crushed at 30° C. to pass through a 60-mesh sieve to obtain tobacco leaf powder.

[0026] (2) Add toluene to the tobacco leaf powder obtained in step 1, and extract with microwave radiation for 30 seconds under the condition of microwave power of 200 watts, cool and filter to obtain a crude extract.

[0027] (3) After concentrating the crude extract obtained in step 2 under reduced pressure, undergo alumina chromatography with petroleum ether as the eluent to obtain β-carotene.

Embodiment 3

[0029] The specific process steps are as follows:

[0030] (1) Tobacco leaves are dried and crushed at 40° C. to pass through an 80-mesh sieve to obtain tobacco leaf powder.

[0031] (2) Add ether to the tobacco leaf powder obtained in step 1, extract under microwave power of 600 watts, microwave radiation for 60 seconds, cool and filter, and obtain a crude extract.

[0032] (3) After the crude extract obtained in step 2 was concentrated under reduced pressure, β-carotene was obtained through silica gel chromatography with petroleum ether as the eluent.

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Abstract

The invention discloses a microwave extraction method for extracting Beta-carotene from tobacco leaves. Firstly, pretreatment is carried out on the tobacco leaves, then organic solvent is extracted under microwave radiation condition, finally decompression, vacuum concentration and column chromatographic isolation and purification are carried out on the extracted solution to prepare the Beta-carotene. The invention has the advantages that: 1. the invention is simple and easy to carry out with rapid speed and high extraction efficiency which is about 25 percent higher than organic solvent and the purity of the Beta-carotene after further column chromatographic isolation and purification can be up to above 98 percent; 2. no saponification is required, which not only reduces steps but also promotes the yield; 3. when the column chromatography isolation and purification is carried out on the Beta-carotene, petroleum ether solvent is taken as eluent and satisfied effect is gained, and the solvent can be recovered easily and reused, thereby having low cost; 4. using value of the tobacco leaves can be developed by implementing the invention, thereby improving the utilization ratio of the tobacco leaves.

Description

technical field [0001] The invention relates to a method for extracting beta-carotene, in particular to a method for extracting beta-carotene in tobacco leaves by using microwave radiation. Background technique [0002] β-carotene is an internationally recognized excellent natural pigment, which widely exists in animals, plants and microorganisms. It is not only an important source of vitamin A in the human body, but also has important physiological functions for the human body. It can prevent and delay And treat certain diseases, especially cancer, and can also improve the body's immune function. Based on these special physiological functions of β-carotene, it has been widely used in food, cosmetics, medicine, flavors and fragrances and other industries. At present, in the β-carotene market, most of the products are chemically synthesized. According to research reports in recent years, natural β-carotene has better anti-cancer effect than chemically synthesized β-carotene,...

Claims

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Application Information

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IPC IPC(8): C07C403/24C07B63/00A24B15/26A24B15/34
Inventor 赵铭钦韩富根刘金霞
Owner 江西润井康生物科技有限公司