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Antimicrobial compositions for inhibiting growth and proliferation of a microbial biofilm on medical devices

一种医疗装置、生物薄膜的技术，应用在抗微生物组合物领域，能够解决延长抗微生物效应、药物不受控制、不足等问题

Inactive Publication Date: 2008-07-02
KANE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the limited mass of drug that can be added may not be sufficient for a prolonged antimicrobial effect, and the release of drug after clinical insertion of the device is rapid and relatively uncontrolled

Method used

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  • Antimicrobial compositions for inhibiting growth and proliferation of a microbial biofilm on medical devices
  • Antimicrobial compositions for inhibiting growth and proliferation of a microbial biofilm on medical devices
  • Antimicrobial compositions for inhibiting growth and proliferation of a microbial biofilm on medical devices

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] The binding effect of embodiment 1-protamine sulfate (PS) and chlorhexidine salt (CHX) Enhanced effect of film-embedded catheter-associated bacteria

[0080] An in vitro microplate assay (microplate assay) was performed to determine the effect of binding of protamine sulfate and chlorhexidine salt on biofilm formation of biofilm-embedded catheter-associated bacteria such as Escherichia coli, Pseudomonas aeruginosa Bacteria and the enhanced growth of Staphylococcus epidermidis. Overnight cultures of each strain grown in Luria-Bertani (LB) or Tryptic Soy Broth (TSB) were used as inoculum. In 12-well microplates, bacteria were grown on colony-forming antigens in the absence and presence (12.5, 25, or 50 μg / ml) of each test compound (PS or CHX), respectively, and together (PS+CHX). (CFA) medium (for Gram-negative) or in TSB (for Gram-positive). Plates were incubated at 37°C for 24 hours. The medium (media) containing planktonic cells in each well was gently removed an...

Embodiment 2

[0081] Example 2 - Protamine sulfate (PS) and chlorhexidine salt (CHX) combined with coated silicon Inhibitory activity of ketone catheters relative to catheter-associated bacteria

[0082] Antimicrobial activity of PS+CHX coated and uncoated 1 cm silicone catheter sections was assessed using the Kirby-Bauer technique as previously described by Sheretz et al. (Antimicrob. Agents. Chemother. 33: 1174-1178, 1989) . Catheters were coated by immersion in a PS (100 mg / ml) + CHX (400 mg / ml) solution followed by drying (as described in US Patent No. 6,475,434). Gas sterilize the catheter with ethylene oxide. Catheter-associated microorganisms such as Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterococcus faecalis, vancomycin-resistant enterococci (VRE), Staphylococcus epidermidis, Staphylococcus aureus, and albicans, grown in nutrient broth for 18 hours at 37°C. Appropriate inoculum of each strain or yeast strain was used to prepare sp...

Embodiment 3

[0085] Example 3 - Protamine sulfate (PS) and chlorhexidine salt (CHX) combined with coated silicon Anti-adhesive effect of ketone catheters on catheter-associated bacteria

[0086] PS+CHX, PS, and CHX coated silicone catheters were tested for activity against bacterial colonization by exposing uncoated and coated sections in triplicate to E. coli, Pseudomonas aeruginosa, and Staphylococcus epidermidis . Silicone catheters were coated with PS (100 mg / ml), CHX (100 mg / ml) and PS (100 mg / ml)+CHX (100 mg / ml) and then gas sterilized with ethylene oxide. The coated catheter sections were incubated in sterile artificial urine at 100 rpm at 37°C for 24 hours prior to challenge with bacteria. After incubation, the catheter section was rinsed with sterile water and incubated in bacterial culture (in BHI medium) at 37°C for 3 hours at 100 rpm. After 3 hours of incubation, the sections were washed gently twice. Each washed section was transferred to a sterile tube containing 1 ml ...

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PUM

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Abstract

The present invention provides compositions for preventing growth and proliferation of biofilm embedded microorganisms on devices comprising: (a) a cationic polypeptide and (b) a bis-gaunide or a salt thereof. The invention further provides methods for preparing medical devices withy such compositions.

Description

[0001] Cross References to Related Applications [0002] This application is related to and claims priority from US Provisional Application No. 60 / 695,546, filed July 1, 2005, and US Provisional Application No. 60 / 742,972, filed December 6, 2005. technical field [0003] The present invention relates to antimicrobial compositions which can inhibit the growth and proliferation of biofilm embedded microorganisms on or in devices, and especially medical devices such as catheters. Background technique [0004] Urinary tract infection (UTI) is the most common hospital-acquired infection, accounting for up to 40% of all hospital-acquired infections. Most cases of UTI are associated with the use of urinary catheters, including transurethral Foley catheters, suprapubic catheters, and nephrostomy catheters. These urinary catheters are inserted in a variety of populations, including the elderly, stroke patients, spinal cord injury patients, postoperative patients, and those with obst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A61L31/16A61L31/08A61L2/16A01P1/00A01N47/44
CPCA61L2/18A61L29/085A01N47/44A61L12/145A61L2300/206A61L2/16A61L29/044A61L2300/252A61L2/22A61L29/16A61L12/082A61K38/17A61L2300/42A61K31/155A61L27/34A61L12/141A61L2300/404A61P13/02A61P31/02A61P31/04Y02A50/30A01N37/46A01N2300/00A01N61/00C08L89/00A61K2300/00
Inventor 斯里尼瓦桑·马德亚斯塔
Owner KANE BIOTECH
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