Method and apparatus for detecting analytes using an acoustic device

An analyte and device technology, which is applied in the field of detecting one or more analytes in fluid samples, can solve the problems of energy consumption, heating conductive fluid, and destroying biological analytes, so as to improve accuracy and sensitivity, and combine strong Effect

Inactive Publication Date: 2008-07-02
BIOSCALE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Electric fields may also dissipate energy and heat conductive fluids (e.g., 0.01M phosphate buffered solution), which is detrimental since heating can destroy biological analytes

Method used

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  • Method and apparatus for detecting analytes using an acoustic device
  • Method and apparatus for detecting analytes using an acoustic device
  • Method and apparatus for detecting analytes using an acoustic device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0165] Example I: General method for functionalizing the surface of flexible wave devices with capture agents

[0166]1. Deposit gold onto the surface of the flexible slab wave device 104 (eg sensor surface 143), clean the gold surface 143 with eg oxygen plasma.

[0167] 2. The ideal surface chemistry (characteristics) of the gold surface 143 should provide 1) reactive groups that prevent non-specific binding and 2) reside on the surface for covalent binding of capture agents. An exemplary surface chemistry (property) of the gold surface 143 is a self-assembled monolayer (SAM) of alkanethiols. SAMs can be formed from a mixture of two alkanethiols; one end containing a reactive group for subsequent covalent attachment to a capture agent, and one end containing a non-reactive group. For example, a C-terminated 11 EG of -alkanethiol 6 -OCH 2 COOH (EG6) and EG 3 Mixture of -OH(EG3). In one embodiment, the flexible plate wave device 104 (specifically, the surface 143 of the d...

Embodiment II

[0169] Example II: Using, for example, the method shown in Figure 3 to detect Escherichia coli O157:H7 in ground beef (Figure 5 contains data representing various concentrations of Escherichia coli)

[0170] 1. Prepare a sample containing E. coli O157:H7 analyte at a concentration greater than about 100 cfu / ml.

[0171] 2. Perform immunomagnetic separation to concentrate the analyte sample solution. This immunomagnetic separation can be performed using various commercially available instruments (eg, Pathatrix from Matrix Microsciences and Bead Retriever from Dynal Biotech) or by manual methods. Exemplary manual methods include:

[0172] a. Resuspend the magnetic beads coated with E. coli antibody (for example, Dynabeads anti-E. coli O157 purchased from Dynal Biotechnology) until the magnetic beads precipitated at the bottom of the test tube are dispersed. Place microcentrifuge tubes on a magnetic plate rack (eg, Dynal MPC-S). Pipette 1-20 μL of magnetic bead stock into this...

Embodiment III

[0191] Embodiment III: adopt, for example method steps shown in Figure 3 detect the prostate-specific antigen (PSA) in human serum

[0192] 1. Preparation of human serum analyte-containing samples obtained by centrifugation of human blood samples.

[0193] 2. Perform immunomagnetic separation to concentrate the analyte sample solution. This immunomagnetic separation can be performed using a variety of commercially available instruments (eg, Pathatrix from Model Microsciences and Bead Retriever from Dynal Biotechnology) or by manual methods. Exemplary manual methods include:

[0194] a. Resuspend the magnetic beads coated with PSA antibody (for example, Dynabeads anti-PSA purchased from Dynamic Biotechnology Company) until the magnetic beads precipitated at the bottom of the test tube are dispersed. Place microcentrifuge tubes on a magnetic plate rack (eg, Dynal MPC-S). Pipette 1-20 μL of magnetic bead stock into this tube (choose magnetic bead stock volume based on desired ...

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Abstract

Methods for detecting analytes are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for the analyte is combined with the sample to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

Description

technical field [0001] The present invention relates to methods of detecting one or more analytes in a fluid sample. Background of the invention [0002] For systems that detect analytes (eg, chemical and biological substances) in liquid media, significant challenges include the concentration of the analyte in the media and the transport of the analyte to the sensor surface. For biological applications, concentration issues often arise due to the potentially low concentration of this analyte. In addition, biological analytes (eg, cells, cell debris, and macromolecules such as proteins and nucleic acids) can be large; therefore, transport problems can arise due to the very slow diffusion of these larger analytes in fluid solutions. [0003] In addition to cells, cell debris, and molecules such as proteins and nucleic acids, detection of small molecule analytes can serve as useful markers for diagnosing disease, monitoring pharmacokinetics of drugs in patients, and screening ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCY02A50/30
Inventor A·斯里瓦斯塔纳W·王M·米勒B·P·马斯特斯V·K·古拉提M·卢恩斯托姆
Owner BIOSCALE
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