Method for extracting astaxanthin, protein and chitin from shrimp shell by utilizing microorganism

A technology of astaxanthin and chitin is applied in the field of microorganisms extracting astaxanthin, chitin polysaccharide and protein from marine animals, which can solve the problems of unusable astaxanthin, reduce degradation cost, achieve good effect and reduce pollution discharge. Effect

Active Publication Date: 2008-07-09
青岛兔爱动物科技有限公司
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  • Application Information

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Problems solved by technology

[0012] [4] Announcement No. 318 of the Ministry of Agriculture of the People's Republic of China

Method used

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  • Method for extracting astaxanthin, protein and chitin from shrimp shell by utilizing microorganism

Examples

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example 1

[0042] After 10 g of raw shrimp shells are coarsely crushed to 0.5-1.0 cm, water is added at a mass ratio of 1:2. Steam sterilization at 121°C for 20 minutes. Inoculate the liquid strains activated for 26 hours at a ratio of 15%, and degrade at 37°C for 36 hours. Centrifuge at 3800r / min for 10min. The supernatant was steam sterilized at 121°C for 20 minutes. After concentrating at 100°C for 10 minutes, it was dried at 105°C to obtain 0.7 g of protein powder. Use 10ml of organic solvent of ethanol:petroleum ether=1:5 to extract astaxanthin from the lower precipitate, and after 1h, the organic solution containing astaxanthin was concentrated under reduced pressure at 45-55°C under a vacuum of 100kp to obtain 0.58mg of astaxanthin. Organic solvent recovery. Wash the degraded shrimp shells and thalline precipitation on a 40-mesh sieve, and dry the eluate naturally to obtain 2 g of biological fertilizer. The remaining shrimp shell residues are dried and used as raw materials f...

example 2

[0044] Raw material, water ratio is the same as example 1. Use pasteurization at 60°C for 30min. Other operations are the same as example 1. Obtain 0.60 mg of astaxanthin, 0.73 g of protein powder, and 2.5 g of biological fertilizer.

example 3

[0046] Raw materials, adding water and sterilization conditions are the same as example 1. The strains were activated for 32 hours and inoculated with 10%. The degradation time is 40h, and the extraction solvent is 95% ethanol, and other operations are the same as in Example 1. Obtained astaxanthin 0.53mg, protein powder 0.77g, biological fertilizer 2.4g.

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Abstract

A method for extracting astaxanthin, protein and chitin from shrimp shells through using microbes comprises following steps: utilizing gram negative bacterium adjacent pseudomonas bacillus (Plesiomonas) to degrade and ferment in the 37 DEG C condition, centrifuging and separating degradation products, concentrating and drying liquid, obtaining albumen powder, immersing solid body with dissolvent, centrifuging again, removing the dissolvent from the liquid, and obtaining the astaxanthin, wherein the medium-lower layer of precipitation is crude chitin and the medium-upper layer of the precipitation is cells and little protein which is used as bio-fertilizer. Compared with an existing technique, the invention has the prominent advantages that multi-purpose use is good, the microbes is applied to degrade and extract astaxanthin from the shrimp shells, the protein and the crude chitin are reclaimed, and the cells and the few protein can be used as the bio-fertilizer, which enables the shrimp shells to be utilized sufficiently and reaches the stage of 'eat and extract completely'. Simultaneously the invention has low pollution, low cost and good effect.

Description

technical field [0001] The invention relates to the technical field of microorganisms extracting astaxanthin, protein and chitin from marine animals. Background technique [0002] Astaxanthin is a ketogenic non-provitamin A carotenoid with the molecular formula C 40 h 52 o 4 . Due to the long conjugated double bond in the molecule, it is very easy to capture electrons, and it is a quencher of singlet oxygen. It has the ability to scavenge free radicals and super antioxidant activity, and the effect can be 500 times that of ordinary vitamin E[1] . Its biological activity is mainly manifested in the prevention of arteriosclerosis, anti-cancer activity, enhancing immunity, and maintaining the health of the central nervous system. It can be used as a feed additive for human advanced nutritional health care and pharmaceutical products and aquatic products, livestock and poultry, especially the aquaculture industry has always been the largest market for astaxanthin. In comme...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12P21/00C12P19/04C05F11/08
Inventor 王际辉高世伟叶淑红辛丘岩
Owner 青岛兔爱动物科技有限公司
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