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Manganese complex as well as preparation method and uses thereof

A technology of complexes and manganese salts, applied in the field of Mn complexes and their preparation, to achieve the effects of high yield, high purity, and excellent inhibitory effect

Inactive Publication Date: 2010-06-16
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, no manganese complexes have been used clinically as anticancer drugs

Method used

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  • Manganese complex as well as preparation method and uses thereof
  • Manganese complex as well as preparation method and uses thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the preparation of complex

[0022] 0.15g MnSO 4 ·H 2 O and 0.4g PHEN were sequentially added to a mixed solvent of methanol and acetonitrile (volume ratio 1:1), stirring continuously. After reacting for about 8 hours, filter, diffuse the filtrate with isopropanol, and let it stand at room temperature. After a few days, yellow crystals can be obtained.

[0023] Elemental analysis: calculated value (experimental value): C 55.25 (55.26); H 3.68 (3.66); N 10.31 (10.33). figure 1 and figure 2 shown.

Embodiment 2

[0024] Embodiment 2, the preparation of complex

[0025] 0.15g Mn(CH 3 COO) 2 4H 2 O and 0.1 g Na 2 SO 4 And 0.4g of PHEN was sequentially added into the mixed solvent of methanol and acetonitrile (volume ratio is 1:1), and the stirring was continued. After reacting for about 8 hours, filter, diffuse the filtrate with isopropanol, and let it stand at room temperature. After a few days, yellow crystals can be obtained.

[0026] Elemental analysis: calculated value (experimental value): C 55.25 (55.26); H 3.68 (3.66); N 10.31 (10.33). figure 1 and figure 2 shown.

[0027] Using other manganese salts (such as manganese nitrate, manganese chloride, manganese acetate, etc.), sulfate anions, and other solvents (such as acetone, DMF, etc.), can obtain similar yellow crystals—that is, the manganese complex of the present invention.

Embodiment 3

[0028] Embodiment 3, cell experiment

[0029] Adherent tumor cells (A-549 (lung cancer) cells, Bel-7402 (liver cancer) cells, HCT (colon adenocarcinoma) cells) in the logarithmic growth phase were selected, digested with trypsin, and RPM11640 with 10% calf serum The culture solution was made into a cell suspension of 5000 cells / mL, inoculated in a 96-well culture plate with 100 μL per well, and cultured at 37°C and 5% CO2 for 24 hours.

[0030] (1) Experimental group:

[0031] Adherent tumor cells (A-549 (lung cancer) cells, Bel-7402 (liver cancer) cells, HCT (colon adenocarcinoma) cells) in the logarithmic growth phase were selected, digested with trypsin, and RPM11640 with 10% calf serum The culture solution was made into a cell suspension of 5000 cells / mL, inoculated in a 96-well culture plate, and inoculated 100 μL in each well, at 37°C, 5% CO 2 Cultivate for 24h.

[0032] Add 10 μL of the sample solution obtained in Example 1 (dissolve the complex crystals obtained in ...

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Abstract

The invention discloses a manganese complex and the preparation method and application of the manganese complex, providing a complex of manganous, 1, 10-phenanthroline and sulfate. The preparation method is to stir the manganous, 1, 10-phenanthroline and sulfate in the solvent and the complex is acquired after reaction. The preparation method for the manganese complex has the advantages of easy access to the composite material, low cost, precipitation of crystal form with high purity and high yield, stable existence in natural state, good water soluble and fat-soluble performance, excellent inhibition effect to cells of lung cancer, liver cancer, colon adenocarcinoma and a plurality of cancers, wide action spectra and application range.

Description

technical field [0001] The invention relates to a Mn complex, a preparation method thereof, and an application of the Mn complex in preparing medicines for preventing and treating cancer and tumor diseases. Background technique [0002] Manganese is an essential trace element for the human body. Manganese participates in the synthesis and activation of many enzymes, participates in the metabolism of sugar and fat in the human body, accelerates the synthesis of protein, vitamin C, and B, catalyzes hematopoietic function, regulates endocrine, and improves immune function. [0003] Research data at home and abroad show that the incidence of cancer in areas deficient in manganese is high, while in areas with high manganese content, the incidence of cancer is low. For example, my country is one of the high incidence areas of esophageal cancer in the world. Manganese in drinking water, grain and vegetables in this area is low. Epidemiological survey results show that the manganese...

Claims

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Application Information

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IPC IPC(8): C07F13/00A61K31/555A61P35/00C07D471/04
Inventor 鲁晓明王硕
Owner CAPITAL NORMAL UNIVERSITY
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