Method for detecting vestigial clenbuterol

A detection method and detection standard technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of high false positive rate, narrow detection range, time-consuming and labor-intensive, etc., and achieve the effect of simple operation and low cost.

Inactive Publication Date: 2008-07-30
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Abstract
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  • Claims
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Problems solved by technology

Although color-mass spectrometry and chromatography have high sensitivity, they require high laboratory conditions, complex detection methods, time-consuming, laborious, and high cost, and are difficult to meet field applic

Method used

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  • Method for detecting vestigial clenbuterol
  • Method for detecting vestigial clenbuterol
  • Method for detecting vestigial clenbuterol

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Embodiment 1

[0020] The experimental materials are as follows:

[0021] Clenbuterol (CL) monoclonal antibody (Abcam)

[0022] Clenbuterol, biotin N-hydroxysuccinimide ester, N,N-dimethylformamide (DMF) (SIGMA Corporation)

[0023] Streptavidin-R-phycoerythrin (phycoerythrinSA-PE) (Invitrogen)

[0024] Fetal bovine serum albumin (Roche)

[0025] Albuterol and ractopamine (Dr. Ehrenstorfer)

[0026] Millipore 96-hole percolation plate (Millipore Corporation)

[0027] MultiScreen(R) Suction Filtration System (Millipore Corporation)

[0028] Suspension chip detection system Bio-Plex Suspension Array Workstation and supporting Bio-Manager 4.0 software and No. 46 coded fluorescent detection microspheres with carboxyl groups (Bio-Rad Company), the characteristics of the microspheres are: polystyrene with a diameter of 5.5 μm Microspheres with carboxyl groups and 2 different red fluorescent dyes on their surface. Each of these two dyes has 10 concentration gradients, so through the combinati...

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Abstract

The invention discloses a method for detecting clenbuterol residues, which comprises the following steps: (1) preparing, identifying and biotinylating clenbuterol-calf serum albumin conjugate; (2) coupling anti-clenbuterol monoclonal antibody on fluorescent microspheres; (3) drawing standard curve for suspension chip detection of clenbuterol residues; (4) testing specificity of suspension chip detection of clenbuterol residues; and (5) measuring the concentration of clenbuterol residues sample. The method of the invention for detecting clenbuterol residues has the advantages of simple operation, rapidness, sensitivity and low cost; and the overall detection process only takes 1-2h.

Description

technical field [0001] The invention relates to a detection method for drug residues in animal food, in particular to a detection method for clenbuterol residues in animal food. Background technique [0002] In recent years, food safety has become a public health issue of common concern, and veterinary drug residues have become an important factor affecting food safety. The domestic market survey found that the problem of antibiotic and hormone residues in meat and dairy products is relatively serious. In recent years, the problem of clenbuterol (CLenbuterol, CL) residues in various animal foods has attracted widespread attention. Clenbuterol is commonly known as "Clenbuterol", and CL is a powerful beta 2 Receptor agonists, which belong to adrenaline-like hormones, abuse can lead to accelerated heart rate, muscle tremors, and metabolic disorders; especially for patients with high blood pressure, heart disease, hyperthyroidism, glaucoma, and prostate hypertrophy, the risk is...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 高志贤刘楠苏璞
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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