Method for detecting if interaction between nucleic acid conjugated protein -target protein exist based on biological chip

Abstract

The invention discloses a method of detecting whether an interaction exists between nucleic acid protein and target protein based on biochip. The method comprises the following steps: (1)adding a plurality of groups of nucleic acid acquisition probes into a biological sample system containing the target protein to form a target-protein-nucleic-acid-conjugated-protein-nucleic-acid-acquisition-probe compound, and the nucleic acid acquisition probes containing at least one section of sequence capable of integrating with the nucleic acid conjugated protein; (2) separating the target-protein-nucleic-acid-conjugated-protein-nucleic-acid-acquisition-probe compound through molecules capable of specifically integrating with the target protein, and then recovering the nucleic acid acquisition probes; (3) carrying out hybridization of the nucleic acid acquisition probes recovered in the step 2 and a plurality of single chain immobilized probes which are fixed on the substrate of the biochip and are corresponding to the nucleic acid acquisition probes, and the nucleic acid sequence of the immobilized probes complementing the corresponding nucleic acid acquisition probes or one chain of the nucleic acid acquisition probes; (4) detecting the hybridization results to see whether the interaction exists between the nucleic acid conjugated protein and the target protein.

Description

technical field

[0001] The invention relates to a method for detecting whether there is an interaction between protein-proteins, in particular to a method for detecting whether there is an interaction between a nucleic acid binding protein and a target protein based on a biochip. Background technique

[0002] Nucleic acid-binding proteins include double-stranded DNA-binding proteins, single-stranded DNA-binding proteins, RNA-binding proteins, and the like.

[0003] Double-stranded DNA-binding protein is a kind of protein molecule or protein molecular complex that can specifically bind to a specific sequence of double-stranded DNA. Double-stranded DNA binding proteins mainly include repressors and operator proteins in prokaryotes and transcription factors in eukaryotes.

[0004] The end of the Human Genome Project (HGP) and the completion of the sequencing of dozens of model organisms marked a gradual shift in the focus of biological research from the era of genomes to the e...

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