Lipopeptid composition with physiological activity and its preparation method and application

A physiologically active and complex technology, applied in the field of lipopeptide complexes, can solve the problems of inability to realize industrialized production, increased purification steps, complicated process routes, etc., and achieves remarkable physiological properties, improved bioavailability, and high yield. Effect

Inactive Publication Date: 2011-01-26
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, it was believed that the higher the purity of the antihypertensive peptide, the better. As a result, the number of purification steps increased, the process route was complicated, and the cost increased, making it impossible to achieve real industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Take 100g of red snail tissue (trunk, liver, reproductive organs) and mash it in a frozen state, add 10 times the amount of 95% ethanol to extract for 1 hour, filter, then stir and extract with 50% acidic ethanol for 3 hours, filter, combine the two extracts, Let cool, precipitate out, pour out the supernatant, concentrate, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, and keep the operating temperature below 40 ℃, vitamin E was added to make the final concentration to 0.03%, concentrated under reduced pressure to remove the solvent to obtain a crude product, which was then separated by sephadex LH20 column chromatography and eluted with butanol-acetic acid-water to obtain a lipopeptide complex with a yield of 25%.

[0051] According to chemical analysis and detection, the obtained lipopeptide complex contains per milliliter: 900 μg of inorganic nitrogen, 280 μg of...

Embodiment 2

[0053] Take all the fresh tissues of 100g of red snails, mash the tissues, add 9 times the amount of 60% ethanol, adjust the pH value to not less than 4.5 with acetic acid, extract in a container with mixing and stirring function for 2 hours, filter, concentrate, let cool and precipitate Precipitate, decant the supernatant, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, keep the operating temperature below 40°C, add 0.02% vitamin E. Concentrate under reduced pressure to remove the solvent to obtain a crude product, which is then separated by sephadex LH20 column chromatography, butanol-water to obtain a lipopeptide complex with a yield of 24.5%.

[0054] According to chemical analysis and detection, the obtained lipopeptide complex contains per milliliter: 850 μg of inorganic nitrogen, 310 μg of inorganic phosphorus, 5.3 μg of unsaturated lipid, and 6.0 μg of hydrolyzed am...

Embodiment 3

[0056] Take 100g of snail tissues (trunk, liver, reproductive organs) and crush them in a frozen state, add 8 times the amount of 80% ethanol, adjust the pH value to not less than 4.5 with acetic acid, stir and extract for 3 hours, filter, and concentrate to 1 / 2 of the original volume 10. Let the precipitate cool down, pour out the supernatant, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, and keep the operating temperature below 40°C. , adding 0.03% vitamin E, concentrating under reduced pressure to remove the solvent to obtain a crude product, which was separated by sephadex LH20 column chromatography, propanol-acetic acid-water to obtain a lipopeptide complex with a yield of 30%.

[0057] According to chemical analysis and detection, the obtained lipopeptide complex contains per milliliter: 804 μg of inorganic nitrogen, 340 μg of inorganic phosphorus, 2.3 μg of unsatur...

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PUM

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Abstract

The invention relates to a natural lipopeptide composite with biological activity extracted from conch, preparation and application in pharmaceutical fields of the same, which pertains to biological and pharmaceutical technical fields. The invention protects a lipopeptide composite with biological activity, preparation and application thereof. The lipopeptide is produced from marine organisms, can be used in treatment and improving hypertension and congestive heart failure.

Description

technical field [0001] The invention relates to a natural physiologically active lipopeptide complex extracted from marine snails, a preparation method of the complex and an application in medicine, belonging to the technical field of biomedicine. Background technique [0002] Hypertension is one of the most common cardiovascular diseases, which can cause damage to the brain, cardiovascular, and kidneys, and is an important factor leading to stroke, heart failure, and coronary heart disease. [0003] Although artificially synthesized antihypertensive preparations have obvious hypotensive effects, the side effects caused by long-term medication have become a lingering problem. The short peptide with antihypertensive effect derived from natural food has the characteristics of significant antihypertensive effect, no toxic and side effects, and high safety, which is more in line with people's health needs. [0004] At present, there are many hydrolyzates and peptides derived fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K2/00C07K1/14C07K1/16A61K38/02A61P9/12A61P9/04
Inventor 刘可春达特森科・卓娅考米沙林科・谢尔盖契克曼・伊万康尼维特・娜塔丽娅莫伊谢耶娃・柳米拉韩利文柳高夫斯卡依士丘克亚斯尼
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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