Technique for suspension cultivation of algam dendrobium nobile embryoid of medicinal effective composition of native plant strain

A technology of Dendrobium officinale and suspension culture, applied in the biological field, can solve the problems of difficult to guarantee the "authentic" standard of medicinal materials, genetic variation, etc., and achieve the effect of expanding market supply and promoting infinite proliferation.

Inactive Publication Date: 2008-08-20
曹兴华 +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Generally speaking, it is difficult to guarantee the "authentic" standard of medicinal materials where genetic variation is likely to occur through the process of dedifferentiation.

Method used

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  • Technique for suspension cultivation of algam dendrobium nobile embryoid of medicinal effective composition of native plant strain
  • Technique for suspension cultivation of algam dendrobium nobile embryoid of medicinal effective composition of native plant strain

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Experimental program
Comparison scheme
Effect test

Embodiment example

[0008] In the following, the present invention will be further described in conjunction with examples of implementation, but the limitations of the present invention will not be limited.

[0009] Accompanying drawing 1, technical flow chart of the technical process flow chart of Dendrobium officinale suspension culture embryoid body production raw plant medicinal effective dry powder

Embodiment 1

[0010] The suspension culture of embodiment 1. Dendrobium officinale germ (seeing accompanying drawing 1)

[0011] 1. Collection and sterilization of Dendrobium officinale explants: Collect adult stem segments of Dendrobium officinale plants grown in the wild, generally with 7-10 node (leaf) stem segments. Rinse with tap water first, carefully peel off the leaves and leaf sheaths with ophthalmic pointed tweezers, cut into sections with 3 to 4 segments with a scalpel, soak in 50% ethanol for 3 to 5 seconds, and put them in fresh water with sterile water. 0.1% mercuric chloride solution, 15 to 17 minutes; then transferred to saturated sodium hypochlorite or saturated calcium hypochlorite solution newly prepared with sterilized water for 7 to 8 minutes; After rinsing with water, soak in sterilized water for about 20 minutes; put it on sterilized absorbent paper to absorb excess water; use a sterilized blade to cut off the stem with nodes, leaving 0.5 cm above and below the nodes,...

Embodiment 2

[0022] Embodiment 2. embryoid body aseptic dry powder production (seeing accompanying drawing 1)

[0023] In order to make the polysaccharide content of Dendrobium officinale more than 16%, the harvest cycle of the finished embryoid body is 10 days. After the harvested embryoid finished product is rinsed with ultra-clean water to remove the medium on the surface, it is placed in a room that has reached the standard GMP certification. Into 55 ℃ of ovens, dry to absolute dry weight (more than 65 ℃, can cause denaturation and transfer loss of many physiologically active ingredients in the Dendrobium officinale embryoid body). After super pulverization, it is packaged quantitatively, and the bacteria are inactivated in a medical cobalt chamber to form sterile dry powder of embryoid bodies.

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Abstract

The invention relates to a technical process flow that an embryoid is directly induced by adopting the strain stem segment dormancy of Dendrobium candidum which is grown in wild field, the one half MS mineral composition by CH1000mg / L (the components of the one half MS. mineral salt are: 2.5 percent sucrose and 0.5 percent maltose which are taken as carbon sources, 5 percent banana juice, B150mg / L, B 650mg / L, NAA and BA appropriate amount of solid culture medium) is subject to continuous screening for 3 to 4 months on a rotary shaking bed at 90 turns / minute, 29 DEG C plus or minus 1 and the natural indoor light condition, so as to obtain the 8.4 to 9.2mg / g.day, the solid-liquid rate achieves 25 to 35 percent, the cultured embryoid cell nucleus DNA is completely the same as the original plant cell nucleus DNA, the polysaccharide content achieves more than 16 percent, the dry yield rate is more than 10 percent, and the technical parameters are stable annually.

Description

Technical field: [0001] The invention belongs to the field of biotechnology. The invention relates to a biotechnology cloning project of higher plants, especially for Dendrobium officinale which grows very slowly in the natural environment, has very harsh requirements on the environmental conditions, and has very low biomass. At present, the natural resources are very exhausted, endangered and precious. of Chinese herbal medicines. technical background: [0002] "The 21st century is the century of biotechnology". With the rapid development of the urbanization process and the continuous growth of population, the continuous degradation of the ecological environment has made many precious Chinese medicinal materials exhausted and endangered. Especially species like Dendrobium officinale, which grow slowly, are very demanding on the environment, and have extremely low biomass, are "bear the brunt". Although since the 1980s, people have attempted to expand the production of De...

Claims

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Application Information

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IPC IPC(8): C12N5/04A61K36/8984A23L1/29A23L2/00C12P19/04A23L33/00
Inventor 曹兴华
Owner 曹兴华
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