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Technique for preparing ribitol by fermentation method

A preparation process, ribitol technology, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., to achieve stable product quality, reduce glycerin content, and low content of by-products such as glycerin

Active Publication Date: 2010-12-08
CHANGMAO BIOCHEMICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, in addition to the above patents, there are no other reports on the preparation of ribitol by fermentation using glucose as raw material at home and abroad.

Method used

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  • Technique for preparing ribitol by fermentation method
  • Technique for preparing ribitol by fermentation method
  • Technique for preparing ribitol by fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: as attached figure 1 shown. Its process steps are:

[0030] (1) Strain selection. The hypertonic yeast Trichosporonoidesoedocephalis ATCC 16958 was selected

[0031] (2) Culture medium preparation.

[0032]

[0033] (3) Strain preparation. The strain was inoculated on a slant, cultured at 30°C±1°C for 4 days, and placed in a 4°C refrigerator for use.

[0034] (4) Seed cultivation.

[0035] Insert the bacterial strain after step 3 slant culture for 4 days into the seed culture solution. Aerobic cultivation was used for seed cultivation, using a 500mL Erlenmeyer shaker flask with a liquid volume of 100mL, a cultivation temperature of 30°C, a shaker rotation speed of 170rpm, and a cultivation time of 45h.

[0036] (5) Fermentation culture.

[0037] a. A 50L fermenter was used for fermentation culture, with a liquid volume of 30L, a tank pressure of 0.5MPa, a culture temperature of 30°C, and a pH of 5.0;

[0038] b. Fermentation method

[0039] The ...

Embodiment 2

[0042] Embodiment 2: as attached figure 1 shown. Its process steps are:

[0043] (1) Selection of strains. With embodiment 1.

[0044] (2) Culture medium preparation. With embodiment 1.

[0045] (3) Strain preparation. With embodiment 1.

[0046] (4) Seed cultivation. Same as implementation 1.

[0047] (5) Fermentation culture.

[0048] a. A 50L fermenter was used for fermentation culture, with a liquid volume of 30L, a tank pressure of 0.5MPa, a culture temperature of 30°C, and a pH of 5.0;

[0049] b. Fermentation method

[0050] Insert the seed culture solution prepared in step 4 into the fermentation medium at a volume ratio of 5%, control the aeration rate during the entire fermentation process to 1vvm, and control the stirring speed at 350rpm 30 hours before fermentation, and control the dissolved oxygen within the range of 65% to 95%. After 30 hours, the stirring speed is reduced to 100-200 rpm, and the dissolved oxygen is controlled at 5%-30%, that is, the s...

Embodiment 3

[0052] Embodiment 3: as attached figure 1 shown. Its process steps are:

[0053] (1) Selection of strains. With embodiment 1.

[0054] (2) Culture medium preparation. With embodiment 1.

[0055] (3) Strain preparation. With embodiment 1.

[0056] (4) Seed cultivation. Same as implementation 1.

[0057] (5) Fermentation culture.

[0058] a. Insert the seed culture solution into the fermentation medium at a volume ratio of 5%, control the ventilation rate to 1vvm during the whole fermentation process, and the stirring speed is 350rpm 30 hours before fermentation, and reduce the fermentation speed to 100-200rpm after 30 hours , so that dissolved oxygen is controlled at 5% to 30%, that is, the staged stirring fermentation method of the present invention is adopted. At the same time, under the premise that the total amount of glucose in the fermentation process is 20%, the glucose concentration is controlled at 5% to 10% in the first 30 hours, and the remaining glucose is...

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Abstract

The invention discloses a preparing technology for preparing ribitol by zymotechnics, which uses glucose as raw material and Trichosporonoides oedocephalis as original strain, and prepares according to the steps of strain preparation, seed cultivation and fermentation. The invention is characterized in that seed solution and fermentation medium are inoculated in a fermentation cylinder in accordance with the volume ratio between 0.1 and 0.01, and are agitated for fermentation cultivation; the fermentation cultivation is performed under the technological condition of the cylinder pressure between 0 and 1MPa, the temperature between 25 and 40 DEG C, the dissolved oxygen between 5 and 95 percent and the pH between 4 and 7; the time for the process of fermentation cultivation is between 80 to160h; the agitation for fermentation process is sectional agitation; and the dissolved oxygen is sectionally controlled through sectionally controlling the speed of agitation. Sectional feeding and purification are implemented. The preparing technology for preparing ribitol by zymotechnics has the characteristics of simple processes, high yield, small material consumption, low content in glyceroland other side products, steady product quality, low preparation cost, and no discharge of three wastes, etc.

Description

technical field [0001] The invention relates to a preparation process of ribitol, in particular to a preparation process of ribitol by fermentation. It belongs to the field of biochemical technology. Background technique [0002] Ribitol is an important starting material for the production of high-value non-natural sugars. It can be directly converted into L-ribose by microbial enzyme reaction or prepared into L-ribulose through microbial dehydrogenation, and then isomerized into L-ribose. In recent years, unnatural L-type sugars and their modified nucleoside analogs have attracted the attention of many researchers because of their potential biological activity and low toxicity, such as rare L-pentose (L-ribose, L-xylose, etc.) have been used to prepare nucleoside analogs with anti-HIV virus effects. Currently, multiple nucleoside analogs of L-ribose are already in phase II or III clinical trials. It can be predicted that with the development of nucleoside analogs such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/645
Inventor 李良智芮新生万屹东元英进尤吉王伟
Owner CHANGMAO BIOCHEMICAL ENG CO LTD
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