Water quality toxicity biosensor microorganism electrode preparation method

Abstract

The invention relates to a microorganism electrode preparation method of a water quality toxicity biosensor, which is characterized in that: 40 to 100 parts of freezing and drying colon bacillus, 20 to 80 parts of microzyme and 40 to 60 parts of nitrosomonas are taken according to weight and the three germs are blended to get a blended germ; then 0.3 to 1.0g of blended germ is taken and added into 200 to 500 Mu L of 0.85 percent chlorine solution and a sterilized toothpick is used for mixing well the germ , thus blended germ solution is available; a diprosopia tape is stuck to the round of the working area of the carbon electrode; 5 to 10 Mu L of blended germ solution is drawn with a liquid relief gun, which is smeared to the working area of the carbon electrode and is air-cured for 0.5 to 2.0h in dark under room temperature; then a Makrolan film is covered on the air-cured blended solution, thus the microorganism electrode is made by fixing the germ through the diprosipia tape, which is at last arranged in a fridge of 4 DEG C for 24 to 48h ready for use. The microorganism electrode obtained from the invention improves largely the sensitivity and repetitiveness of the water quality toxicity analysis of the biosensor. The microorganism electrode preparation method of the water quality toxicity biosensor has the advantages of easy techniques and easy operation, can realize the reusage of the biosensor electrode, thus largely reducing the instrument analysis cost.

Description

technical field [0001] The invention relates to a method for preparing a microbial electrode of a CellSense biosensor used for water quality toxicity analysis. The microbial electrode prepared by the method can not only improve the toxicity analysis performance of the CellSense biosensor, but also reduce the analysis cost. It belongs to the technical field of environmental monitoring. Background technique [0002] At present, there are mainly two types of methods for the determination of wastewater toxicity, one is physical and chemical methods, and the other is biological methods. Traditional physical and chemical analysis methods can quantitatively analyze the content of main components in pollutants, but they cannot directly and comprehensively reflect the comprehensive impact of various toxic substances on the environment. Biological testing can make up for the lack of physical and chemical testing methods, so in water pollution research, it has become one of the import...

AI Technical Summary

Problems solved by technology

At present, in the research of CellSense biosensor, single strains such as Escherichia coli (E.coli), Pseudomonas putida (Pseudomonas putida), Vibrio fischeri (V.fischeri), or mixed strains such as activated sludge have been used. (Activated sludge) as the research report of the tested microorganisms (Marinella et al. : 41-54; Wang et al. Chinese Chemical Letters 2008, 19: 211-214), but in actual water quality toxicity analysis, because wastewater is often a complex pollution system in which multiple pollutants coexist, and different types of microorganisms Therefore, the CellSense biosensor based on a single strain has the disadvantages of poor sensitivity and reproducibility of toxicity analysis

At the same time, from the perspective of environmental pollution ecotoxicology, the toxicity evaluation based on a single strain has the limitation that it cannot objectively reflect the true toxic effect of pollutants

The CellSense biosensor using activated sludge as the tested microorganism has the characteristics of wide detection range of pollutants and true toxicity analysis results, but due to the large differences in the composition and structure of the bacterial flora of different activated sludge, it has caused differences among laboratories. The toxicity test results are difficult to compare with each other. The above problems greatly limit the development of this type of microbial sensor

[0005] The microbial electrodes currently used by CellSense biosensors are prepared by pre-printing adhesives around the working area of ​​the carbon electrodes and pasting polycarbonate membranes to directly cover and fix the tested microorganisms. The microbial electrodes prepared by this method are disposable. The electrode is discarded after completing a water toxicity analysis, which leads to high analysis costs and affects its promotion and application

[0006] Therefore, in order to improve the performance of CellSense biosensor toxicity analysis and reduce the cost of analysis, the development and immobilization of test microorganisms need further research