Coupling process of marsh gas fermentation and culture of autotrophic freshwater microalgae

A technology for biogas fermentation and microalgae cultivation, which is applied in the fields of biogas fermentation and self-supporting freshwater microalgae cultivation, can solve the problems of high cost, waste gas and waste liquid, etc., and achieve the effects of reducing production costs, improving the environment and increasing the economy

Inactive Publication Date: 2008-10-15
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aiming at the high cost of autotrophic freshwater microalgae culture medium and the waste gas and waste liquid of biogas fermentation, a method of constructing a coupled culture system for the two is proposed

Method used

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  • Coupling process of marsh gas fermentation and culture of autotrophic freshwater microalgae

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Embodiment 1

[0027] The microalgae Neochloris oleoabundans (purchased from UTEX algae collection center in the United States, UTEX#1185) in this example is an autotrophic freshwater alga, and the total oil content in the BG11 medium can reach more than 45% of the dry biomass, which is rich in oil Microalgae. Biogas and biogas slurry were used as all nutrient sources for Neochloris oleoabundans, and algal cells were cultured in a small indoor tubular photobioreactor at room temperature. The algae have been experimentally confirmed to survive under these conditions. The steps are as follows:

[0028] A: Biogas fermentation—mix waste biomass such as human and animal manure and domestic waste with water at a ratio of 1:19, add 30% anaerobic activated sludge, and anaerobic fermentation at 35°C for 25 days to obtain biogas and biogas slurry, biogas It is introduced into the photobioreactor through the pipeline, and the biogas slurry is treated as a culture medium.

[0029] B: Treatment of bio...

Embodiment 2

[0035] Using photoautotrophic Ankistrodesmus pseudobraunii (purchased from UTEX algal species collection center, UTEX#LB 1380) as microalgae species, using biogas and biogas slurry as all nutrient sources for the algae, small-scale pipeline photobiology at room temperature The algal cells are grown in the reactor. The algae have been experimentally confirmed that they can survive in biogas slurry as the only medium, and are oil-rich microalgae. The steps are as follows:

[0036] A: Biogas fermentation—mix water hyacinth, human and animal manure, domestic waste and other waste biomass with water at 1:20, add 10% by weight to anaerobic activated sludge, anaerobic fermentation at 35°C for 20 days, Obtain biogas and biogas. The biogas is introduced into the photobioreactor through the pipeline, and the biogas slurry is treated as a culture medium.

[0037]B: Treatment of biogas slurry - take the biogas slurry after biogas fermentation, remove residual solid particles by absorbe...

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Abstract

The invention belonging to the environmental protection and new technique energy source field discloses a coupling method for biogas fermentation and autotrophy type fresh water micro-algae culture. The invention is characterized by adopting the method of simultaneously carrying out biogas fermentation and autotrophy type fresh water micro-algae culture including biogas fermentation, marsh liquid processing, autotrophy type fresh water micro-algae inoculation and biogas introduction, control of autotrophy type fresh water micro-algae culture, and harvest of autotrophy type fresh water micro-algae culture. The invention adopts the coupling method of biogas fermentation process and phototrophy type fresh water micro-algae culture. The marsh liquid is taken as the culture medium of autotrophy fresh water micro-algae, and the CO2 in the biogas is taken as the CO2source needed by autotrophy type fresh water micro-algae culture, thereby greatly reducing the production cost of autotrophy type fresh water micro-algae and solving the problems of biogas purification and marsh liquid aftertreatment.

Description

Technical field: [0001] The invention relates to a method for coupling the utilization of biogas fermented biogas liquid, the purification of biogas and the cultivation of autotrophic freshwater microalgae, which is specially used for biogas fermentation and cultivation of autotrophic freshwater microalgae. Background technique: [0002] Autotrophic microalgae fix CO during photosynthesis 2 , and using inorganic nitrogen, phosphorus and other inorganic salts as nutrient sources, it has the characteristics of high photosynthesis efficiency, strong environmental adaptability, short growth cycle and high biological yield, and is an ideal biomass energy material. The comprehensive development and utilization of microalgae, especially oil-rich microalgae with high oil content, has become an important measure in many countries to save energy, turn harm into profit and protect the environment. Various inorganic salts of autotrophic microalgae account for more than 60% of its total...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12N1/12C12R1/89
CPCY02E50/343Y02E50/30
Inventor 欧阳平凯韦萍何若平李环周华应汉杰黄和
Owner NANJING UNIV OF TECH
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