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Process for extracting chlorogenic acid and separating protein and small peptide form sunflower meal

A technology for separating protein and chlorogenic acid, which is applied in the fields of extracting chlorogenic acid, separating protein and small protein peptides, and achieving the effects of reducing production costs, preventing oxidation of phenolic hydroxyl groups, and saving solvent consumption.

Inactive Publication Date: 2011-05-11
黄磊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese Patent: Application No. 200510047693.0 provides a method for continuously extracting chlorogenic acid and sunflower protein from sunflower meal, which has the following technical defects: 1. Brine immersion extraction method, this extraction method requires very high temperature and concentration of brine, otherwise it will easily cause protein denaturation in sunflower meal and affect the leaching rate, and the leaching rate is very low; 2. The sunflower meal after soaking is ground with a colloid mill Direct separation, since the ground substance is an emulsion, it will block the resin when directly passing through the macroporous resin, which will affect the adsorption; 3. The eluent is heated for a certain period of time to remove the solvent contained in it, which will lead to green Ortho acid oxidation
[0004] There are also technologies such as "A Method for Extracting and Purifying Chlorogenic Acid from Sunflower Meal" (Application No. 200510094347.8) in the prior art, but most of these technologies use stirring The method of extraction or extraction requires a large amount of solvent and high extraction cost, and there is no separation and extraction of denatured protein and binding protein in sunflower meal, which fails to make the best use of sunflower meal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Get the sunflower meal powder 100 grams that passes through 40 mesh sieves through pulverizing, adjust the 45% ethanol aqueous solution wet method of pH4.5 with stabilizer and pack in the percolator. The percolator is a glass column, Φ40mm×320mm, material height 188mm, soaked for 5h. The stabilizer is a mixed solution of ascorbic acid, acetic acid and water, wherein the ratio of ascorbic acid: acetic acid is 3:2, and the ratio of mixed acid: water is 1:1. The percolation liquid is packed with the column liquid, the percolation flow rate is 2ml / min, and 600ml of the percolation liquid is collected. It is determined that the extraction rate of chlorogenic acid is more than 98%, and the chlorogenic acid contained in the first 400ml accounts for more than 92% of the total. The first 400ml of percolation liquid is concentrated to no alcohol with a rotary evaporator, about 50ml, and the last 200ml is used for the next batch of charging. Take 100 grams of treated polyamide o...

Embodiment 2

[0028] Get 1000g of sunflower meal powder that passes through 30 meshes through pulverization, and adjust the 55% hydroalcoholic solution of PH5.0 with stabilizer and load in the percolator by wet method. The percolator is plexiglass, with a diameter of Φ80mm×700mm, soaked for 12h. The stabilizer is a mixed solution of ascorbic acid, phosphoric acid and water, wherein the ratio of ascorbic acid:phosphoric acid is 2:3, and the ratio of mixed acid:water is 1:2. The percolation solution is percolated with the column liquid, the flow rate is 25ml / min, 6000ml of the percolation solution is collected, the first 4000ml is concentrated under reduced pressure to recover ethanol until the concentrated solution is alcohol-free, about 200ml. The last 2000ml is used for the next batch of filling. Take 600g of processed 80-120 mesh polyamide, put it into a Φ60mm×900mm chromatography column by wet method with 20% ethanol aqueous solution, balance it with 20% ethanol aqueous solution, put th...

Embodiment 3

[0032] Take 10 kilograms of sunflower meal powder through 20 mesh through crushing, and use stabilizer to adjust PH4.0 50% ethanol aqueous solution and put it into the percolator by wet method, the percolator is plexiglass Φ200mm×1000mm, the stabilizer is ascorbic acid and acetic acid and water A mixture of ascorbic acid: acetic acid 1:1, mixed acid: water 1:1. Carry out percolation with column packing liquid at a speed of 300ml / min, collect 60000ml of percolation liquid, recover ethanol under reduced pressure in the first 40000ml to about 1000ml without alcohol, and use the last 20000ml for the next batch of charging. Take 4000g of treated polyamide of 50-80 mesh, wet-pack the column with 10% ethanol aqueous solution, the chromatographic column Φ150mm×1500mm, equilibrate with 10% ethanol aqueous solution, put the concentrated solution 1000ml on the top of the polyamide, and use 10% ethanol Elute with aqueous solution, collect the 4-14 times eluate, concentrate under reduced p...

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Abstract

The invention relates to a technology for extracting cholorogenic acid from sunflower meals and separating proteins and small protein peptide, which adopts the method of ethanol water percolation to extract and adopts a chromatography method of polyamide column to purify the cholorogenic acid, meanwhile, the residues of the sunflower meals which separates the cholorogenic acid are separated again to extract the useful protein isolate and small protein peptide thereof, thus the added value of the sunflower meals are largely added. The technology of the invention can prevent the oxidation of the cholorogenic acid in the process of extraction, which improves the purity and yield of the cholorogenic acid and reduces the production cost at the same time.

Description

technical field [0001] The invention relates to the comprehensive utilization technology of sunflower meal, in particular to a process for extracting chlorogenic acid, protein isolate and small protein peptide from sunflower meal. Background technique [0002] Sunflower meal is the residue left after the sunflower seeds have been hulled to extract the oil. The active ingredients of sunflower meal are sunflower protein and crude fiber containing chlorogenic acid and other substances. Among them, the protein content is as high as 30% to 48%, and the chlorogenic acid content is 1% to 3%. Chlorogenic acid is a phenylpropanoid substance formed by plants in the process of aerobic respiration. It has various pharmacological effects such as choleretic, antibacterial, antihypertensive, increasing white blood cells and stimulating the central nervous system. At the same time, it also has a strong Antioxidant, it has high application value in cosmetics, food, and medicine. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14C07K1/30C07C67/48A61K36/28
Inventor 黄磊
Owner 黄磊