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Preparation method and use method of polylactic acid tree-like molecule-modified magnetic nano particles

A technology of magnetic nanoparticles and dendrimers, applied in the field of bioengineering, can solve problems such as efficiency limitation and achieve the effect of good solubility

Inactive Publication Date: 2008-11-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the slow release of magnetic nanoparticles modified by dendrimers is accompanied by the function of genes or drugs, and there is still a problem of efficiency limitation. After being modified by linking with polylactic acid, the slow release function can be significantly enhanced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 0.2g ferroferrogen tetroxide magnetic nanoparticles whose surface is rich in hydroxyl, add 3ml of 3-amino-triethoxysilane in water, and react at 70°C for 24 hours. After the reaction, magnetic separation and washing are performed to remove excess 3- Amino-triethoxysilane to obtain ferromagnetic particles with amine-based surface. Disperse the product in 20ml of water, add 0.2g of ester-terminated PAMAM dendrimers, ultrasonically treat at 50°C for 30 minutes, and wash with water for 3 times through magnetic separation to obtain the 0.5th generation of dendrimer-modified magnetic nanoparticles with a large number of esters on the surface base group. Dissolve the magnetic nanoparticles modified by G0.5 generation dendrimers in anhydrous methanol, and add them dropwise to 10 times the volume of ethylenediamine methanol solution at a rate of 1 drop / second. Stir continuously. Stirring was continued for 48 hours, methanol and excess ethylenediamine were distilled off under re...

Embodiment 2

[0030] Take 0.2 g of the fifth-generation polylactic acid dendrimer-modified magnetic nanoparticles prepared in Example 1, disperse them in 20 ml of water, add 2.0 ml of ethylenediamine, ultrasonicate at 50°C for 30 min, and then wash with water for 3 times through magnetic separation , to remove excess ethylenediamine monomer. Dendrimer-modified magnetic nanoparticles on the amine-based surface were obtained with a yield of 95%.

Embodiment 3

[0032] Take 0.2 g of the fifth-generation polylactic acid dendrimer-modified magnetic nanoparticles in Example 1, disperse them in 20 ml of water, and heat them to 100° C. under reflux for 2 hours under alkaline conditions (pH=9.0) to carry out hydrolysis reaction. After the reaction is completed, wash with water three times through magnetic separation to obtain a composite material formed of magnetic nanoparticles on the surface of carboxyl groups and dendrimers, and the surface contains a large amount of carboxyl groups (-COOH). can interact with the NH on the surface of the peptide molecule + 3 The group reacts to form CO-NH 2 Peptide bond connection, yield up to 95%. Then it is co-cultured with tumor cells, and electron microscopy or confocal microscopy can be used to confirm that the magnetic nanoparticles modified by polylactic acid dendrimers enter the tumor cells along with the peptide molecules.

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Abstract

The invention discloses a preparation and an application method of magnetic nanoparticles modified by polylactic acid dendritic molecules in the technical field of biological engineering. The preparation method is that a coprecipitation method is adopted to prepare the magnetic nanoparticles; amino silane modifies the magnetic nanoparticles; generation 0.5 of polyamide dendritic molecules grows on the surfaces of the magnetic nanoparticles; the first generation to the tenth generation of the dendritic modules grow on the surfaces of the magnetic particles; the obtained magnetic nanoparticles modified by the dendritic molecules are dissolved in trichloromethane and the polylactic acid is added in the ratio of 2 to 1, constantly stirred and reacting, and then reduced pressure distillation is carried out to remove the trichloromethane and obtain thick liquid, thus obtaining the magnetic nanoparticles coated with the polyamide dendritic molecules and connected with the polylactic acid. The magnetic nanoparticles modified by the polylactic acid dendritic molecules and prepared by the invention have the advantages of the dendritic molecules and the magnetic nanoparticles, and the slow-release characteristics of the polylactic acid, thereby having the application value in gene or drug delivery.

Description

technical field [0001] The invention relates to a method in the technical field of bioengineering, in particular to a preparation and application method of polylactic acid dendrimer-modified magnetic nanoparticles with slow-release function. Background technique [0002] Due to their superparamagnetic properties and good biocompatibility, magnetic nanoparticles have broad application prospects in the medical field. Such as nuclear magnetic resonance contrast agent, GMR biosensor, gene and drug delivery carrier, hyperthermia under external magnetic field, etc. However, a large number of studies have found that the efficiency of magnetic nanoparticles to cross the cell membrane is not high, and they are easy to aggregate and not easy to disperse. How to aggregate in specific target cells or organs is a challenging problem. [0003] Dendrimers are a new type of dendritic polymers, which are monodisperse polymer materials with a spherical three-dimensional structure, and struct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/00H01F1/00C04B35/628B22F1/02A61K49/06
Inventor 崔大祥潘碧峰高峰贺蓉
Owner SHANGHAI JIAO TONG UNIV
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