Method for extracting alkaloid valid target from hooked uncaria leaf, with hook stem branch or full grass and uses thereof

A technology for effective parts and alkaloids, which is applied in the field of extracting effective parts and uses of alkaloids from Uncaria leaves, hooked stems or whole plants

Inactive Publication Date: 2009-01-14
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is currently no effective part of alkaloids extracted from Uncaria leaves, hooked stems or whole plants with a total alkaloid content greater than 50% as the main raw material for the treatment or prevention of neurodegenerative diseases such as Alzheimer's and cardiovascular and cerebrovascular diseases such as hypertension. Oral preparations for diseases are launched

Method used

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  • Method for extracting alkaloid valid target from hooked uncaria leaf, with hook stem branch or full grass and uses thereof
  • Method for extracting alkaloid valid target from hooked uncaria leaf, with hook stem branch or full grass and uses thereof
  • Method for extracting alkaloid valid target from hooked uncaria leaf, with hook stem branch or full grass and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Add 75% ethanol solution of 8 times the weight of Uncaria uncariae stems and branches to extract by percolation, filter, combine the filtrates, recover under reduced pressure until there is no alcohol smell, and obtain the concentrated extract of Uncaria uncaria uncariae stems and branches. Dilute the concentrated extract with water to a medicinal solution 6 times the amount of Uncaria uncariae stems and branches, and filter to obtain a filtrate. The filtrate was passed through an adsorption column filled with DM-130 macroporous resin, eluted with 6 times the amount of 80% ethanol, and the solvent was recovered under reduced pressure to obtain a thick paste, which was dried to obtain a total alkaloid content of 76.0% ( HPLC method measures) dry cream, wherein the content sum of rhynchophylline, isorhynchophylline, dehydrorhynchophylline and isodehydrorhynchophylline is 40.1% (HPLC method measures). The dry paste is pulverized by ultrafine grinding technology, take 1 par...

Embodiment 2

[0070] Add 75% ethanol solution 10 times the weight of Uncaria leaf coarse powder for percolation extraction, filter, combine the filtrates, recover under reduced pressure until there is no alcohol smell, and obtain the concentrated extract of Uncaria leaves. Dilute the concentrated extract with 10% ethanol to 8 times the medicinal solution of Uncaria leaves, filter to obtain the filtrate. The filtrate was passed through an adsorption column filled with D101 macroporous resin, rinsed with 6 times the amount of 30% ethanol until the effluent was colorless, then eluted with 6 times the amount of 75% ethanol, and the solvent was recovered under reduced pressure. Get the thick paste, the dry paste that contains total alkaloids is 70.3% (HPLC method survey) through drying, and wherein the content of rhynchophylline, isorhynchophylline, dehydrorhynchophylline and isodehydrorhynchophylline The sum is 42.4% (measured by HPLC method). Mix 1 part of crushed dry cream powder with 0.1 pa...

Embodiment 3

[0072] Add Uncaria leaf coarse powder 12 times the weight of 75% ethanol solution to extract by cold soaking for 12 hours, filter, combine the filtrates, recover under reduced pressure until there is no alcohol smell, and obtain the concentrated extract of Uncaria leaves. Dilute the concentrated extract with 20% ethanol to 6 times the amount of Uncaria leaves, and filter to obtain a filtrate. Pass the filtrate through the adsorption column filled with AB-8 macroporous resin, rinse with 6 times the amount of 30% ethanol until the effluent is colorless, then elute with 6 times the amount of 55% ethanol, and collect the 55% ethanol Eluent, decompression reclaims solvent, gets thick paste, and thick paste is the dry paste that contains total alkaloids of 76.6% (HPLC method survey) through drying, and wherein rhynchophylline, isorhynchophylline, dehydrorhynchophylline and The sum of the contents of isodehydrorhynchophylline is 41.5% (measured by HPLC method). After the dry paste i...

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Abstract

The present invention belongs to the technical field of medicine, and discloses a method or an application extracting the valid target of alkaloid from ramulus uncariae cum uncis leaves, stems with hooks or whole grass. The preparation method of the invention comprises: adding hydrous alcoholic solution to ramulus uncariae cum uncis leaves, stems with hooks or whole grass to perlocate or extract with hot reflux; filtering; decompressing; recycling to nonalcoholic taste; adding water or alcoholic solution to concentrated extracting solution; filtering; making filtrate run through a adsorption column filled with adsorbing material; washing the adsorption column with water to the point that effluent liquid is colorless before elution; eluting with 20 to 75 percent of alcoholic solution; collecting eluting liquid; decompressing; recycling solvent and obtaining thick paste; drying and obtaining the valid target of alkaloid. The method does not use toxic organic solvent and does not polluteenvironment, which has the advantages of less time consumption, high efficiency, simple extracting technology and low cost. The invention can realize industrialization and meet environment protectionrequirement. The extracted valid target of alkaloid can remedy or prevent senile dementia and other neurodegenerative diseases, high blood pressure and other cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method and application for extracting effective parts of alkaloids from Uncaria leaves, hooked stems or whole plants, and specifically relates to extracting biological components from Uncaria leaves, hooked stems or whole plants. The method of the effective part of the base and the application of the effective part in the preparation of medicines for treating or preventing neurodegenerative diseases such as senile dementia and cardiovascular and cerebrovascular diseases such as hypertension. Background technique [0002] With the aging of the social population, Alzheimer's disease, cerebrovascular disease, and malignant tumors have been listed as the three major diseases of the elderly. Alzheimer's disease (AD) is a primary brain degenerative disease of unknown etiology, and it is a frequently-occurring and common disease in the elderly. The disease was first described by Alois...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61K9/48A61K9/20A61K9/16A61P25/28A61P9/12A61P9/00A61K127/00A61K135/00
Inventor 袁丹吴春福鹿野美弘杨静玉马斌
Owner SHENYANG PHARMA UNIVERSITY
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