Artificial cultivation method for halimasch sporophore, culture medium and uses thereof

A technology for artificial cultivation of Armillaria sprouts and artificial cultivation is applied in the field of artificial cultivation of Armillaria fruiting bodies, can solve the problems such as failure to form industrialization of fruiting body cultivation, and achieves the effects of short cultivation period, simple operation and high fruiting body yield.

Inactive Publication Date: 2009-01-14
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no research reports on the cultivation of Armillaria Armillaria as an edib...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Strains: Armillaria armillaria strains, from the China General Microorganisms Collection and Management Center, preserved on the slant of PDA medium, and the preservation number is 5.130.

[0028] Preparation of Armillaria liquid seed: 100ml of liquid seed culture medium in 250ml triangular bottle, liquid seed culture medium formula volume ratio component is as follows: 2.0% glucose, 0.6% peptone, 0.06% thiamine, 1.0% ethanol , potassium dihydrogen phosphate 0.3%, pH 6.0, water 96.04%. Sterilize at 121°C for 30 minutes, cool to room temperature, inoculate two pieces of Armillaria strains (the size of soybean grains) stored on the slant of PDA medium, and cultivate at 25°C on a rotary shaker with a speed of 120rpm and a temperature of 25°C for 7 ~10 days, become Armillaria liquid seeds.

[0029] The preparation of medium: medium formula: get 25% wheat bran and cornmeal by weight, 75% water, the ratio of wheat bran and cornmeal is 10:1;

[0030] Cultivation of the fruit...

Embodiment 2

[0032] Basically the same as in Example 1, the difference is the strain: Armillaria strain, which comes from the China General Microorganisms Collection and Management Center, and is stored on a PDA medium slant, and the preservation number is 5.146.

[0033] The preparation of culture medium: culture medium formula: get 18% wheat bran, 82% water by weight.

Embodiment 3

[0035] Basically the same as in Example 1, the different strains: Armillaria strains, from the China Agricultural Microbiology Preservation Management Center, preserved on the slope of PDA medium, and the preservation number is 50801.

[0036] It is the preparation of culture medium: culture medium formula: get 23% wheat bran and rice flour by weight, 77% water, the ratio of wheat bran and rice flour is 3: 1.

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Abstract

The invention discloses a method for cultivating a fruiting body of a honey fungus, comprising the steps of: providing a honey fungus strain; preparing a culture medium: with compositions in a formula, by weight ratio: 18 to 25 percent of cereal crop and 75 to 82 percent of water; cultivating the honey fungus in the seed culture medium, inoculating the acquired honey fungus seed onto the prepared culture medium, and transferring the seed to the conditions of temperature of between 17 and 19 DEG C, relative humidity of 90 percent, and 12h scattered light each day for cultivation when the seed is in dark culture at a temperature of between 15 and 30 DEG C for 25 to 35 days, and the fruiting body begins to take shape after 10 to 15 days, and is picked at maturity, and a fungus embryo of the picked honey fungus fruiting body can serve as a functional feedstuff additive after being dried, the fungus embryo is extracted with water, the extract serves as a raw material for production of drugs, and the extracted waste residues serve as feedstuff. The method has the advantages of short outbreak cycle of the fruiting body, high output, wholly comprehensive utilization of the fungus embryo and non waste.

Description

Technical field: [0001] The invention relates to a method for artificially cultivating fruit bodies of Armillaria armillaria, belonging to the technical field of edible fungus cultivation. Background technique: [0002] Armillaria mellea (Vahl ex Fr.) Quel, also known as hazel mushroom, belongs to Basidiomycotina, Agaricaceae, Agaricaceae, Armillaria genus, a well-known medicinal and edible fungus. Armillaria armillaria are distributed in all continents in the world, and mainly distributed in Heilongjiang, Jilin, Inner Mongolia and Taiwan and other provinces and regions in my country. Wild Armillaria mostly grows after the rain in summer and autumn, and grows in clusters at the base of old or dead trees. It can also parasitize on living trees, and it can also grow in large quantities on bushes and grasslands. Folks in Northeast my country have long had the habit of collecting Armillaria fruiting bodies to eat and treat diseases. Regular consumption can prevent vision loss, ...

Claims

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Application Information

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IPC IPC(8): C05F11/00A23K1/16A61K36/07A01G1/04A23K10/30
Inventor 程显好刘林德刘静葛宜和高兴喜张秋胜缪静屈慧鸽
Owner LUDONG UNIVERSITY
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