Method for breeding cotton with greensickness-resistance

A technology for resistance to verticillium wilt and verticillium wilt, applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problem of low yield of disease-resistant varieties, large blindness in material selection or elimination, and years The problems of large differences, etc., achieve the effects of stable resistance (resistance) to Verticillium wilt, strong resistance (resistance) to Verticillium wilt, and increased soil bacterial load.

Active Publication Date: 2009-01-21
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI +1
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  • Application Information

AI Technical Summary

Problems solved by technology

After the 1970s, the occurrence of Fusarium wilt of cotton gradually increased, and breeding for resistance to Fusarium wilt began. However, the breeding technology basically used natural disease-susceptible test fields for disease resistance identification. The identification results of hybrid offspring materials are inaccurate, and the selection or elimination of materials is relatively blind
After the 1990s, the occurrence of Verticillium wilt became more and more serious, and there was an urgent need to breed varieties resistant to Verticillium wilt. However, methods similar to those for resistance to Fusarium wilt were still used in the identification and selection of disease resistance. The breeding efficiency was low, and the selection of offspring materials was inaccurate. Poor disease resistance and lower yields of resistant varieties
In the late 1990s, Bt genetically modified insect-resistant cotton was commercially planted in a large area in my country. Although it has a significant control effect on target pests such as cotton bollworm, the transgenic insect-resistant cotton currently on the market is basically not resistant to Verticillium wilt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Breeding of Verticillium wilt-resistant cotton variety Zhongzhi 372

[0020] In 1993, the Xinxiang Scientific Research Pilot Base of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences established a conventional cotton Verticillium wilt disease nursery. The average temperature of the base in July and August in summer exceeded 28°C for less than 20 days. In the disease nursery, the fluffy soil residue with strong pathogenicity and strong pathogenicity after processing the wool cottonseed collected from different cotton producing areas where Verticillium wilt occurs seriously is crushed and mixed as Verticillium wilt maintenance and renewal strains, which are used for the short-term prevention of verticillium wilt. Cashmere residue powder not less than 1200g / m 2 Soil and bacteria-carrying residues are evenly applied to the field to ensure that the number of soil pathogens in the disease nursery is above 200 per gram of soil, and the above...

Embodiment 2

[0023] Example 2 Breeding of Zhimian No. 2, a cotton variety resistant to Verticillium wilt and resistant to cotton bollworm

[0024] In 1995, the Bt gene cloned and transformed by Mr. Guo Sandui, Institute of Biotechnology, Chinese Academy of Agricultural Sciences, was introduced into the Zhongzhi 372 of Example 1 through the pollen tube passage method, and the single gene containing the anti-cotton bollworm gene was selected and bred through Hainan. 65 plants were planted in the disease nursery of the Xinxiang Scientific Research Pilot Base of the Chinese Academy of Agricultural Sciences in 1997. Individual plant isolation and Verticillium wilt resistance breeding were carried out. 106 individual plants were selected for insect resistance and Verticillium wilt resistance. 100 individual plants were selected and planted in the disease nursery in 1999, and 12 individual plants with better performance were selected. In 2000, they entered the base for preliminary experiments, and...

Embodiment 3

[0025] Example 3 Breeding of New Cotton Variety "Xinzhiza No. 2" with High Verticillium Wilt Resistance

[0026] Using the Zhongzhimian No. 2 (GK44) selection line of Example 2 as the female parent and carrying out a large number of orthogonal and reverse cross matching combinations with many line materials, and at the same time carrying out a comparison test with the current main hybrid varieties. In 2003, from 50 Among the combinations, 10 better combinations were determined. In 2004, on the basis of multi-point comparison tests, it was finally determined that Zhimian No. 2 (GK44), a high-yielding, disease-resistant and insect-resistant strain, was selected as the female parent, and the stability and adaptability The new line Xin 59-4 with strong sex, good agronomic traits, and excellent fiber quality was selected as the male parent to breed a new combination of fusarium wilt resistance, Verticillium wilt resistance, and high-yield hybrid cotton, named Xinzhiza 2. In 2007, i...

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PUM

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Abstract

The invention relates to a verticillium wilt resistant cotton breeding method, which comprises the following steps: (1) a disease nursery is established and the pathogenic seeds are annually updated; (2) the seeds are soaked, infected with pathogen and sowed; (3) the verticillium wilt resistant cotton material is bred. The method can also identify and screen out a cotton variety with compatible resistance by introducing a Bt gene. The variety which is accurately bred by the method and the breeding process provided by the invention has the advantages of strong and stable verticillium wilt resistance, high yield, wide applicability and remarkable economic benefits.

Description

technical field [0001] The invention belongs to the field of crop breeding, in particular to a cotton breeding method for Verticillium wilt resistance. Background technique [0002] Verticillium wilt is one of the most important diseases of cotton, known as the "cancer" of cotton, and it was introduced to China in 1935. Since Fusarium wilt was brought under control in the late 1980s, Verticillium wilt has become the number one disease of cotton. Since the 1990s, the disease has spread to all major cotton producing areas in my country, and the rate of diseased plants in seriously diseased cotton fields is often as high as 80%, resulting in serious yield loss. In 1993, 1995 and 1996, Verticillium wilt occurred in a large area continuously. Among them, in 1993, the national cotton Verticillium wilt disaster area reached 40 million mu (2.6667 million hm2), and the loss of lint was 2 million tons (100 million kg), especially in the northern cotton area. In many cotton fields, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/02A01G7/00C12N1/20C12Q1/02
Inventor 张永军李修立李方顺吴孔明
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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