Method for covalent coupling protein on amino magnetic bead surface
An amino magnetic bead and surface covalent technology, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of magnetic bead aggregation and low coupling efficiency, and achieve good repeatability and repeatability , Improve the effect of protein coupling efficiency
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[0025] Example: Amino Magnetic Beads Conjugated Sheep Anti-Mouse Polyclonal Antibody
[0026] (1) Activation of magnetic beads: chemical groups containing maleimide are generated on the surface of magnetic beads. Activation methods include:
[0027] (1.1) Magnetic bead pretreatment: Take 100 mg of amino magnetic bead colloid solution and settle under the action of a magnetic field (magnetic separation) for 10 minutes, remove the supernatant, and wash the settled magnetic beads twice with magnetic bead activation buffer, each time using 5 ml.
[0028] (1.2) Suspend the washed magnetic beads with 1 ml of activation buffer, add the activator Sulfo-SMCC, and suspend and react at room temperature for 15 minutes. The reaction concentration of Sulfo-SMCC is 1 mM.
[0029] (1.3) 10 microliters of 1M glycine buffer at pH 7.3 was added, and the reaction was suspended at room temperature for 10 minutes to terminate the activation reaction.
[0030] (1.4) Magnetic separation for 10 minu...
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