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Method for enrichment of n-3 polyunsaturated fatty acid glyceride from fish oil

A technology for unsaturated fatty acids and fatty acid glycerides, which is applied in the separation/purification of carboxylic acid compounds, organic chemistry, fermentation, etc., can solve problems such as esterification and enrichment, achieve good food safety, and is conducive to human digestion and absorption. The effect of promoting the development of marine economy

Inactive Publication Date: 2011-07-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the separation of EPA and DHA in fish oil and the enrichment of esterification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Accurately weigh 10g of refined fish oil sample, add 150mL of 2mol / L NaOH ethanol aqueous solution, the ratio of ethanol / water (v / v) is 6:1, protect with nitrogen, saponify at 50°C for 60min, add 100mL of n-hexane to extract unsaponified The hydrated layer was acidified with 100mL 3mol / L HCl, and then the free n-3PUFA was extracted with n-hexane 100mL×3, washed with water until neutral, and washed with anhydrous Na 2 SO 4 , N 2 Dry the solvent to obtain n-3PUFA, the hydrolysis rate of fish oil by chemical method is 95.5%, the yield of EPA is 92%, and the yield of DHA is 90%.

[0023] Put 2 g of the n-3PUFA mixed solution containing EPA and DHA obtained after hydrolysis of the fish oil on a C18 column for chromatographic separation, the mobile phase is methanol / water, 70-100% methanol gradient elution, and the eluent is collected in parts, using Rapidly analyze the chromatographic samples by mass spectrometry, and use HPLC to detect the purity of EPA and DHA collected ...

Embodiment 2

[0026] Accurately weigh 10g of refined fish oil sample, add 150mL of 2mol / L NaOH ethanol aqueous solution, the ratio of ethanol / water (v / v) is 9:1, protect with nitrogen, saponify at 60°C for 90min, add 100mL of n-hexane to extract unsaponified The hydrated layer was acidified with 100mL 3mol / L HCl, and then the free n-3PUFA was extracted with n-hexane 100mL×3, washed with water until neutral, and washed with anhydrous Na 2 SO 4 , N 2 The solvent is blown dry to obtain n-3PUFA, and the hydrolysis rate of fish oil by chemical method is 92%, the yield of EPA is 90%, and the yield of DHA is 88%.

[0027] Put 2 g of the n-3PUFA mixed solution containing EPA and DHA obtained after hydrolysis of the fish oil on a C18 column for chromatographic separation, the mobile phase is methanol / water, 70-100% methanol gradient elution, and the eluent is collected in parts, using Rapidly analyze the chromatographic samples by mass spectrometry, and use HPLC to detect the purity of EPA and DHA...

Embodiment 3

[0030] Accurately weigh 10g of refined fish oil sample, add 150mL of 2mol / L NaOH ethanol aqueous solution, the ratio of ethanol / water (v / v) is 9:1, protect with nitrogen, saponify at 50°C for 90min, add 100mL of n-hexane to extract unsaponified The hydrated layer was acidified with 100mL 3mol / L HCl, and then the free n-3PUFA was extracted with n-hexane 100mL×3, washed with water until neutral, and washed with anhydrous Na 2 SO 4 , N 2 Drying the solvent to obtain n-3PUFA, the hydrolysis rate of fish oil by chemical method is 94%, the yield of EPA is 91%, and the yield of DHA is 89%.

[0031] Put 2 g of the n-3PUFA mixed solution containing EPA and DHA obtained after hydrolysis of the fish oil on a C18 column for chromatographic separation, the mobile phase is methanol / water, 70-100% methanol gradient elution, and the eluent is collected in parts, using Rapidly analyze the chromatographic samples by mass spectrometry, and use HPLC to detect the purity of EPA and DHA collected...

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Abstract

The invention relates to a method for enriching n-3 polyunsaturated fatty acid (n-3PUFA) from fish oil, which belongs to the food organism and functional health product technical field. The method takes the fish oil as a raw material, uses the chemical hydrolysis method to hydrolyze the fish oil, respectively obtains high-purity eicosapentaenoic acid (EPA) and high-purity docosahexaenoic acid (DHA) from the hydrolyzed fish oil through separation and purification, uses glycerol and the purified EPA or DHA as raw materials and utilizes lipase to respectively catalyze and synthesize an EPA glyceride product or a DHA glyceride product in an organic phase. The products are mainly n-3PUFA triglyceride; the purity of the products is high; and the concentration of EPA triglyceride in the EPA glyceride is between 90 and 95 percent, and the concentration of DHA triglyceride in the DHA glyceride is between 92 and 94 percent. The high-purity EPA triglyceride product and the high-purity DHA triglyceride product prepared by the method well improve the application pertinence of different health care efficacies of natural fish oil products and improve the functional health care value of the fish oil products.

Description

technical field [0001] The invention provides a method for enriching n-3 polyunsaturated fatty acid glycerides from fish oil. The n-3 polyunsaturated fatty acid glycerides are abbreviated as n-3PUFA glycerides, and belong to the technical field of food biology and functional health care products. Background technique [0002] The n-3 polyunsaturated fatty acids (n-3PUFA) in fish oil have important biological significance in the human body, and the physiological activities of the characteristic fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) It has been clear that EPA has physiological activities such as preventing coronary heart disease, lowering blood pressure, eliminating fatigue, preventing atherosclerosis and cerebral thrombosis, and anti-cancer. DHA can significantly promote the intellectual development of infants, improve brain function, and improve memory. EPA and DHA is a good base material for the production of cardiovascular disease preventio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C07C57/02C07C51/48
Inventor 陈洁何志勇陈小娥陶冠军唐学燕全文琴
Owner JIANGNAN UNIV
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