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Compound successive crop-resistance micro-ecological formulation special for cucumber and special bacterial strain and use thereof

A micro-ecological preparation and anti-repeated cropping technology, which is applied in the field of cucumber-specific composite anti-repeated-cropping microecological preparations, can solve the problems of growers' economic losses, disease spread, and increased pathogenicity, and achieve the protection of human health, prevention of environmental pollution, and enhanced The effect of resilience

Inactive Publication Date: 2009-01-28
北京康泰正茂农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to long-term continuous cropping and the use of pesticides, pathogenic bacteria develop drug resistance, and the pathogenicity is enhanced, causing the disease to spread and rampant, thus bringing serious economic losses to growers.

Method used

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  • Compound successive crop-resistance micro-ecological formulation special for cucumber and special bacterial strain and use thereof
  • Compound successive crop-resistance micro-ecological formulation special for cucumber and special bacterial strain and use thereof
  • Compound successive crop-resistance micro-ecological formulation special for cucumber and special bacterial strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1, the preparation of compound anti-cropping micro-ecological preparation

[0011] 1. Acquisition of bacterial strains for controlling cucumber cropping disease

[0012] Two high-efficiency strains for controlling cucumber replanting disease were isolated and screened from healthy plants and roots of cucumber replanting and severe disease fields—Trichoderma harzianum numbering KTF002 and paenibacillus polymyxa numbering KTB002.

[0013] The isolation and screening methods of Trichoderma harzianum KTF002 and Paenibacillus polymyxa KTB002, which are effective strains for controlling cucumber cropping disease, are as follows:

[0014] (1) Separation

[0015] Trichoderma harzianum isolation plate medium: potato 200g, glucose 20g, agar 20g, water 1000ml, sodium propionate 1g. Then pack it into Kelvin bottles or test tubes, place in a sterilizer, sterilize at 0.11MPa pressure, 121°C for 30min, cool down and make a slope for use.

[0016] Paenibacillus polymyxa i...

Embodiment 2

[0066] Embodiment 2, preparation of compound anti-cropping probiotics

[0067] The preparation method of the composite anti-cropping micro-ecological preparation is the same as in Example 1, except that the solid preparations of Trichoderma harzianum KTF002CGMCC No.2563 and Paenibacillus polymyxa KTB002 CGMCC No.2565 are mixed in a mass ratio of 10:3 to prepare Composite cropping-resistant probiotics for preventing and controlling cucumber gray mold, downy mildew, blight, and fusarium wilt. Each gram of the composite anti-cropping probiotics contains 50 chlamydospores of Trichoderma harzianum KTF002 CGMCC No.2563 100 million cfu / g, the spores of Paenibacillus polymyxa KTB002 CGMCC No.2565 are 15 billion cfu.

Embodiment 3

[0068] The field experiment effect of the compound anti-heavy cropping micro-ecological preparation of embodiment 3, embodiment 1

[0069] The test site was selected at the Yanqing Base of Beijing Lvfulong Vegetable Company. The base had a history of growing cucumbers for five consecutive years, and the incidence of Botrytis cinerea was severe over the years. During the test period, the greenhouse temperature was 10-20°C and the humidity was above 85%. Botrytis occurs naturally. Cucumber Jinchun No. 3 (Beijing Botong Agronomy Science and Technology Center) was planted in mid-December in 2006, with a row-to-plant spacing of 0.6m×0.3m, and a plastic greenhouse plot with flat terrain, uniform soil properties, uniform and medium fertility, and relatively uniform disease incidence was selected.

[0070] Establish 2 processes, process 1 is to carry out foliage spraying each 1 time of the compound anti-heavy cropping preparation of embodiment 1 respectively on March 14 and April 3 ne...

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Abstract

The invention discloses a composite anti-continuous cropping microbial ecological agent specially used in cucumber, special bacterial strain thereof and the preparation. The composite anti-continuous cropping microbial ecological agent takes bacillus polymyxa KTB002 CGMCC No.2565 and trichoderma hazianum KTF002 CGMCC No.2563 as active ingredients. The content of the bacillus polymyxa KTB002 CGMCC No.2565 is 50 hundred million cfu / g preparations; the content of trichoderma hazianum KTF002 CGMCC No.2563 is 5 hundred million cfu / g preparations. The composite anti-continuous cropping microbial ecological agent of the invention is highly active, non-toxic, non-polluting, not only can prevent and control diseases caused by cucumber continuous cropping, but also can increase the output of cucumbers, improve quality and enhance stress resistance of cucumber plant, is beneficial to reducing the usage of chemical pesticide and the residues of chemical pesticide in agricultural products, and is important to protect human health and prevent environmental pollution.

Description

technical field [0001] The invention relates to a special compound anti-heavy-cropping microbial ecological preparation for cucumbers, special bacterial strains and applications thereof. Background technique [0002] Cucumber is rich in a variety of vitamins and proteins, has multiple functions such as nourishing blood and appetizing, increasing appetite, and beautifying, and it is available all year round. Therefore, the healthy growth of cucumber is related to the health of eaters. However, in recent years, with the adjustment of the agricultural industry structure, the planting area of ​​cucumbers in protected areas has been continuously expanding, but the yield has not been significantly increased, and has a downward trend year by year. The main reason is that diseases are caused, one of the causes of diseases It is heavy cropping. Cucumber cropping diseases mainly include damping-off, downy mildew, fusarium wilt, gray mold, and root rot. More than 50%, or even no har...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P3/00
Inventor 王琦张云龙
Owner 北京康泰正茂农业科技股份有限公司
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